Assessing the effect of different shapes of glyco-gold nanoparticles on bacterial adhesion and infections

Abstract

Achieving selective and sensitive carbohydrate-protein interactions (CPIs) using nanotechnology is an intriguing area of research. Here we demonstrate that the different shapes of gold nanoparticles (AuNPs) functionalized with monosaccharides tune the bacterial aggregations. The mechanism of aggregation revealed that the large number of surface interactions of rod shaped mannose-AuNPs with E. coli ORN 178 compared with spherical and star-shaped AuNPs exhibited higher avidity and sensitivity. Moreover, such sensitive binding can be used for effective inhibition of bacterial infection of cells.

Exploring the Influence of Shapes and Heterogeneity of Glyco‐Gold Nanoparticles on Bacterial Binding for Preventing Infections

“…The rod‐shaped mannose‐AuNPs displayed 5‐ to 10‐fold higher bacterial aggregation at different concentrations than their spherical counterparts. These results correlate well with previous bacterial binding studies . R‐1 revealed similar or nearly 1.5‐fold higher bacterial aggregation relative to hetero‐mannose AuNPs.…”

Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish

“…The required mannose-linker was synthesized as described in the literature 28 . The fluorescent-linker ( 8 ) was synthesized from ( 3 ), which was obtained by treating with thioacetic acid and azo-isobutyronitrile (AIBN), followed by one-pot de-acetylation and de-esterification to yield compound 5 .…”

Thio‐Bromo “Click” Reaction Derived Polymer–Peptide Conjugates for Their Self‐Assembled Fibrillar Nanostructures

“…1 g of the as‐synthesized thioacetylated polymer 3 (0.83 mmol, M n,GPC = 1200 g mol −1 , Đ = 1.05) was dissolved in 20 mL of dry methanol solvent and the solution was purged thoroughly under N 2 atmosphere for 20 min followed by addition of 0.24 mL of NaOMe solution (4.17 mmol). [ 34,35 ] The reaction mixture was allowed to stir at room temperature for 10 h and then was dried under rotary evaporator. 50 mL of dry CH 2 Cl 2 were added to this crude and the organic layer was thoroughly washed with 50 mL of degassed aqueous solution of 1 n HCl (3×), and was finally dried over anhydrous Na 2 SO 4 followed by evaporation in rotary and vacuum pump.…”