Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay

Abstract

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. im of this study to determine genotoxic effect of methomylbased pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 -96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12‰, 18‰ and 16‰; at concentration at 3.2ppm is 33‰, 26‰ and 29‰; at concentration at 4.2ppm is 41‰, 38‰ and 46‰; at concentration at 6.5ppm is 68‰, 81‰ and 82‰; at concentration 8.7ppm is 133‰, 130‰ and 137‰; at concentration 10ppm is 163‰, 166‰ and 156‰. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.

In Vitro and FTIR Spectroscopy: Local Black Fruit Seed Extract as Antibacterial Aeromonas Hydrophila

“…The results obtained are then concluded with a comparison of the inhibitory capacity of the active compound against the bacterial activity. The inhibition zone formed after the incubation period is measured and recorded [17]. [18]classified the criteria for bacterial inhibition by suggesting that the inhibition zone diameter of 0 mm does not inhibit, 5-10 mm is weak, 11-20 mm is strong, > 22 mm is very strong.…”

Current Approaches to and Future Perspectives on Methomyl Degradation in Contaminated Soil/Water Environments

“…The toxicological impacts of methomyl on aquatic animals, amphibians, land mammals, and humans are presented in Table 1. Reduction in the efficiency of the food chain in a Cr/Dg system [34] Different aspects of methomyl toxicity on tilapia as a model aquatic organism have been studied [15,35,36]. A high concentration of methomyl was found to drastically change biochemical and histological activities in tilapia.…”

“…A high concentration of methomyl was found to drastically change biochemical and histological activities in tilapia. Islamy et al [35] reported that genotoxicity increased as the concentration of methomyl increased (0-10 mg L −1 ). Moreover, prolonged exposure to methomyl at a concentration above 20 mg L −1 can result in injury to testicular tissue [36,37].…”

Profile of Amino Acid and Fatty Acid from Different Type of Worm as Feed of Broodstock Candidates for Domesticated Uceng (Nemacheilus fasciatus)

“…The research was conducted in April-Juni 2018. Maintenance and treatment activities of test fish are carried out at Reproduction Laboratory, Fisheries and Marine Science, University of Brawijaya, Malang, according to published methods (Islamy et al, 2017;Kilawati and Islamy, 2019). Testing of amino acid profiles is carried out at Saraswati Indo Genetech Laboratory, Jakarta.…”