Background: The objective of this study was to compare the levels of heavy metals (Pb, Hg, and Cd) and metallothionein (MT) in the gills and stomach of two species of mussels ( Crassostrea iredalei and Crassostrea glomerata), and to observe the ability of the mussels to absorb the heavy metals Pb, Hg and Cd present in the water. Methods: The mussels were obtained from Mayangan, Kenjeran and Gresik ports, East Java, Indonesia. MT levels were determined using ELISA. Heavy metal levels of Pb, Hg and Cd were assayed using atomic absorption spectrophotometry. Results: The levels of Pb and Cd in water were below the maximum permissible levels for local water quality standards. By contrast, the level of Hg in the water was above the maximum permissible levels for water quality standards. At Mayangan Port (Station 1), the level of Pb was higher than Hg and Cd. Levels of MT and heavy metals varied greatly among of C. iredalei and C. glomerata individuals, but were always higher in the gills than in the stomach. The highest MT level (160,250 ng/g) was observed at Kenjeran Port (Station 2). MT levels were shown to be significantly associated with heavy metal level ( P<0.0001). Conclusions: This result indicates that MT may be responsible for the sequestration of these heavy metals, as has already been observed in terrestrial animals.

Abstract. Hertika AMS, Supriatna, Darmawan A, Nugroho BA, Handoko AD, Qurniawatri AY, Prasetyawati RA. 2021. The hematological profile of Badher fish (Barbonymus altus) to evaluate water quality in the Badher Bank Conservation Area, Tawangrejo Village, Blitar District, East Java, Indonesia. Biodiversitas 22: 2532-2541. Badher fish (Barbonymus altus) is endemic to the Brantas River. After years presumably extinct, the Badher fish was found again in 2010 in the Brantas River, Tawangrejo Village, Binangun Sub-district, Blitar District, East Java, Indonesia. A conservation area, called a Badher Bank, was established in the location. However, human activities, such as ecotourism and agricultural waste runoff, have changed water quality and increased water pollution in the Badher Bank Conservation Area. This research objective was to observe the hematological profile of badher fish and a set of water quality parameter measurements as an environmental factor that affected fish life. Data were obtained from four research stations with three replications every two weeks from August to October 2020 at the Brantas River streamline inside the Badher Bank Conservation Area. The Canonical Correspondence Analysis (CCA) method was applied to determine the correlation between water quality and hematological profile. According to the data analysis, the water quality at Stations 1-3 in the research location was normal, and Station 4 was polluted. The analysis also showed that the low dissolved oxygen and high ammonia affected the hematological conditions of Badher fish. All water quality parameters affected the condition of leucocytes, erythrocytes, and hemoglobin in moderate concentrations. Besides that, micronuclei and hematocrit conditions were strongly influenced by ammonia, temperature, pH, and current velocity in high concentrations, while Dissolved Oxygen (DO) and Total Suspended Solid (TSS) affected low concentrations.

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. im of this study to determine genotoxic effect of methomylbased pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 -96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12‰, 18‰ and 16‰; at concentration at 3.2ppm is 33‰, 26‰ and 29‰; at concentration at 4.2ppm is 41‰, 38‰ and 46‰; at concentration at 6.5ppm is 68‰, 81‰ and 82‰; at concentration 8.7ppm is 133‰, 130‰ and 137‰; at concentration 10ppm is 163‰, 166‰ and 156‰. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.

Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10 th , 12 th and 14 th , with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields.

The province of South Kalimantan has the largest peat swamp that is named Danau Panggang Swamp with an area of 5,390.7 Ha. This swamp has ecological, economic and potential functions of a large fishery resource. The purposes of this study are to evaluate the exploitation rate of Snake Head Fish (Channa striata) in Danau Panggang Swamp, by population dynamic analysis using FISAT program of Von Bertalanffy method.  The results showed that the growth pattern of Snake Head Fish (Channa striata) has the tendency to grow negative allometric with 63.4 cm of long infifer (L¥), growth rate (k) 0.15 per year, theoretical age at the time of the fish is equal to 0 (t0) is -1.48257 years. While the natural mortality rate (M) was 0.43, the mortality rate due to catch (F) of 0.69/year and the total mortality rate  (Z) of 1.12/year, and the rate of exploitation value E = 0.62.  These results indicated that the exploitation rate of Head Snake Fish (Channa striata) in Danau Panggang swamp has a tendency to overfishing.