Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum

Abstract

Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10 th , 12 th and 14 th , with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields.

Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei

“…In our previous study [15], we reported on P. cruentum growth and extracellular polysaccharides or exopolysaccharides production from this species. Microalgae growth was observed every day for 14 days of culturing, with the provision of 1ml/L of Fe, silicate, and vitamins, and 24-h continuous lighting.…”

“…After 14 days of microalgae culturing, with densities of 15%, 20% and 25%, respectively, we had produced exopolysaccharides of 10,000 mg/L, 12,000 mg/L and 14,000 mg/L, respectively. The Fourier transform infrared spectroscopy (FTIR) test reported in our previous study showed that exopolysaccharides of P. cruentum consist of dominant bonds, namely phenol bonds and polysaccharide bonds [15].…”

Extracellular Polysaccharide from <em>Porphyridium cruentum (purpureum) S</em>timulates the Non-Specific Immune Activities on White Shrimp (<em>Litopenaeus vannamei</em>)

“…The FTIR test reported in our previous study showed that in EPS there were dominant bonds, namely phenol bonds and polysaccharide bonds [16]. Polysaccharides, one of the ingredients that can be used to produce immunostimulant, are classified into carbohydrates.…”

“…In our previous study [16], we reported about Porphyridium growth and Extracellular Polysaccharides (EPS) production from this species. Microalgae growth was observed every day for 14 days of culture with the provision of 1ml/L of Fe, silicate, and vitamins and 24-hours continuous lighting.…”

“…Transformed Infrared (FTIR) method to determine the chemical groups of the compounds contained in EPS as initial information about the chemical composition of EPS[16].4.4. Shrimp TreatmentThe test animals were white shrimps (Litopenaeus vannamei), 7 cm in length and 5 gram in weight.…”