Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding

Cui, Xinyun; Li, Weijie; Wei, Jianhe; Qi, Yanfeng; Li, Rong‐Tao; Yang, Yun; Shi, Yuhua; Meng, Xiangying; Mi, Yaolei; Huot, Theang; Sun, Wei; Zheng, Xiongfei

doi:10.3389/fphar.2020.00244

Cited by 14 publications

(5 citation statements)

References 30 publications

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“…Although surveys of wild edible plants and fungi sold in Southeast Asia are rare, some research effort has been put into studying the socioeconomic aspects of “green” open markets in general [ 44 – 47 ] as well as the contamination of plants with heavy metals [ 48 ] or parasites and pathogens [ 49 – 51 ]. Some studies from Southeast Asia performed in open air markets concern plant genetic resources from a single species, genus or family; the identification of the main cultivated plants (e.g., [ 52 – 56 ]); or medicinal plants [ 24 , 57 ].…”

Section: Introductionmentioning

confidence: 99%

Wild food plants and fungi sold in the markets of Luang Prabang, Lao PDR

Łuczaj

Lamxay

Tongchan³

et al. 2021

J Ethnobiology Ethnomedicine

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Background Open air markets hold an important position for ethnobiologists. In Southeast Asia, they are seriously understudied, in spite of their incredible biocultural diversity. In order to fill this gap we recorded plants and fungi sold in the open air markets of Luang Prabang, Lao PDR. Methods The markets were visited 38 times in four seasons: the dry season, early monsoon, mid-monsoon, and end-of-monsoon, at least 8 times per season. All items were photographed and voucher specimens were collected. Fungi were identified using DNA barcoding techniques. Results We recorded 110 species of wild edible plants and 54 species of fungi, including 49 wild-collected species. The sold plants included 86 species of green vegetables, 18 species of fruits and 3 species of flowers. Products from woody species constitute around half of all taxa sold. These include the young shoots of tree leaves, which are used for salads—an interesting feature of Lao cuisine. A large number of extremely rare Russula, with no reference sequences represented in databases or even species unknown to science is present on sale in the markets. Conclusions Luang Prabang markets are some of the richest in species of wild edible plants and fungi in Asia, and indeed in the whole world. It is worth pointing out the exceptionally long list of wild edible mushrooms which are sold in Luang Prabang (and probably elsewhere in Laos). We view the Morning Market of Luang Prabang as a cultural treasure that unites the traditions of eating a large number of living species with very diverse flora and fauna. Measures should be taken to strike a balance between local foraging traditions and nature conservation priorities.

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Section: Introductionmentioning

confidence: 99%

Wild food plants and fungi sold in the markets of Luang Prabang, Lao PDR

Łuczaj

Lamxay

Tongchan³

et al. 2021

J Ethnobiology Ethnomedicine

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“…For the 19 species with existing DNA barcodes and sequences, we expanded the available data by generating additional DNA sequences using a broader array of DNA barcoding markers. According to the literature, some DNA barcodes were accessible for the following species: viz; R. serpentina ; trnL-trnF, ITS2, matK, rbcL and trnL (Eurlings et al (2013); Cahyaningsih, et al (2022); C. nagbettai ; rbcL, matK, psbA-trnH, rpoC, rpoB, psbKpsbI, atpF-atpH and RPB2 (Kurian, et al (2020); (Table 1); O. indicum ; ITS2, psbA-trnH (Cui, et al (2020); G. indica ; rbcL, ITS, psbA-trnHf, RpoB-trnCGAR, Matk (Seethapathy et al 2018); C. circinalis ; ITS2 (Gao, et al (2010); S. roxburghii ; ITS1 and ITS2; psbM, rbcL, psbH, rpoC2 (Osathanunkul, et al (2021); H. parviflora; MatK, rbcL (data unpublished); V. chinensis; rbc However, in our study, we developed additional markers beyond those that currently exist. We observed that certain existing markers were not amplified in our samples, possibly due to sequence mixing, as evidenced in species such as R. serpentina, G. indica, C. circinalis, S. roxburghii , and P. santalinus (Table 1).…”

Section: Discussionmentioning

confidence: 99%

DNA barcode developement based on chloroplast and ITS genes for species identification of endangered and threated species of Western Ghats, India

Fatima,

Srividya,

Singh

2024

Preprint

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Accurate identification is crucial for conserving species, especially in regions such as the Western Ghats, where trade poses a significant threat to endangered and threatened forest species. Traditional morphology-based identification can be challenging and time-consuming, leading to inaccuracies, especially with similar-looking species or dried specimens. Therefore, DNA barcoding offers a potent solution for precise species identification to address illicit trade and address impactful conservation measures. DNA barcoding is a taxonomic technique that uses standardized short DNA sequences to differentiate and classify species. This approach is especially valuable when morphological characteristics alone are insufficient for accurate species identification. In this study, we focused on the development of a DNA barcoding system for the efficient and accurate identification of threatened and endangered important forest species of Western Ghats Karnataka. To develop the DNA barcoding system, a multilocus approach utilizing sixteen standard DNA barcoding markers was used. A total of 47 threatened and endangered forest species from the Western Ghats were selected for this study. Using a larger number of markers to develop DNA barcodes led to the most precise species identification rates. Moreover, the wide availability of DNA barcode databases allows for quick and accurate species identification. In our study, we observed the highest amplification rates for rbcL1 (40 species), psbtrnH2 (36 species), and PsbA-trnH1 (33 species). DNA amplification varied from 11.76% to 94.11%. Notably, the highest DNA amplification rates were detected forA. wightii(94.11%) andA. hondala (92.34%), both of which belong to the Arecaceae and Passifloraceae families, respectively. Sequencing success rates ranged from 37.5% to 100%. This study will aid in the development of a database of available threatened forest species in western Ghats Karnataka and other regions.

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“…The stems of Leea manillensis are being sold as a commercial herb in the domestic market of Cambodia (Cui et al 2020).…”

Section: Economic Importancementioning

confidence: 99%