Assessing the immunomodulatory potential of high‐molecular‐weight extracts from mushrooms; an assay based on <scp>THP</scp>‐1 macrophages

Velde, Jan Van de; Wilbers, Ruud H. P.; Westerhof, Lotte B.; Raaij, Debbie R. van; Stavrakaki, Ioanna; Sonnenberg, A.S.M.; Bakker, J.; Schots, A.

doi:10.1002/jsfa.6726

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…[72,73] LPS-stimulated THP-1 cells are commonly used to evaluate the immunomodulatory activity of natural products. [74][75][76] LPS is present in the outer membrane of Gram-negative bacteria and is a key pathogen-associated molecular pattern that interacts with TLR4 receptors to trigger the release of cytokines by monocytes and macrophages. [77][78][79] We observed that LPS-stimulated THP-1 cells produced TNF-α and IL-12, and that FE extracts at all tested concentrations significantly reduced the levels of these cytokines.…”

Section: Effect Of Fee Constituents On Il-12 Production From Lps-stim...mentioning

confidence: 99%

Phytochemical study and immunomodulatory activity of Fraxinus excelsior L.

Qasaymeh

Rotondo

Seidel

2023

Journal of Pharmacy and Pharmacology

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Objectives Fraxinus excelsior L. (FE) is traditionally used to treat inflammatory and pain disorders. This study aimed to identify the constituents of FE leaves and evaluate the effects of its n-hexane (FEH), ethyl acetate (FEE), methanol (FEM) extracts and constituents on the viability of THP-1 cells and their ability to release pro-inflammatory cytokines. Methods THP-1 cell viability was assessed using an MTT assay. The immunomodulatory activity was evaluated by measuring tumour necrosis factor-alpha (TNF-α) and interleukin 12 (IL-12) released by lipopolysaccharide-stimulated THP-1 cells using enzyme-linked immunosorbent assays. Key findings Triterpenes, tyrosol esters, alkanes, phytyl and steryl esters, pinocembrin and bis(2-ethylhexyl)phthalate were isolated from FE. The tyrosol esters showed no significant effect on THP-1 cell viability. FEH, FEE, FEM, and pinocembrin, ursolic acid, oleanolic acid had IC50 values of 56.9, 39.9, 124.7 µg/ml and 178.6, 61.5 and 199.8 µM, respectively. FE extracts, ursolic acid, oleanolic acid and pinocembrin significantly reduced TNF-α/IL-12 levels. The tyrosol esters did not significantly affect TNF-α/IL-12 production. Conclusions FE was able to reduce pro-inflammatory cytokine production indicating a mechanistic focus in its use for inflammation and pain. Further investigations are warranted to unravel the mode of action of the tested constituents and discover other potentially active compounds in FE extracts.

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Section: Effect Of Fee Constituents On Il-12 Production From Lps-stim...mentioning

confidence: 99%

Phytochemical study and immunomodulatory activity of Fraxinus excelsior L.

Qasaymeh

Rotondo

Seidel

2023

Journal of Pharmacy and Pharmacology

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“…The pattern recognition receptors activate the innate immunity after pathogen recognition, while Tolllike receptors activate the pathways coordinating with the innate immunity and trigger the immunity response [114]. Velde et al studied the immunomodulatory potential of the Agaricus subrufescens and Coprinus comatus species of mushroom using the THP-1 cells [116]. Various other researchers also evaluated the upregulation of genes using the extracts of Ganoderma lucidum, L. edodes, Agaricus bisporus, and A. subrufescens [117][118][119][120].…”

Section: Mushrooms As Immunomodulatorsmentioning

confidence: 99%

“…The levels of GM-CSF, IL-6, and IL-1 β also showed a spike after per se administration of PG101. The fruiting bodies from the G lucidum, after treatment, showed a rise in levels of IL-1β, TNF-α, and IL-6 by Velde et al studied the immunomodulatory potential of the Agaricus subrufescens and Coprinus comatus species of mushroom using the THP-1 cells [116]. Various other researchers also evaluated the upregulation of genes using the extracts of Ganoderma lucidum, L. edodes, Agaricus bisporus, and A. subrufescens [117][118][119][120].…”

Section: Mushrooms As Immunomodulatorsmentioning

confidence: 99%

“…Velde et al studied the immunomodulatory potential of the Agaricus subrufescens and Coprinus comatus species of mushroom using the THP-1 cells [ 116 ]. Various other researchers also evaluated the upregulation of genes using the extracts of Ganoderma lucidum , L. edodes , Agaricus bisporus , and A. subrufescens [ 117 , 118 , 119 , 120 ].…”

Section: Pharmacological Actions Of Mushroommentioning

confidence: 99%

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Narrative Review: Bioactive Potential of Various Mushrooms as the Treasure of Versatile Therapeutic Natural Product

Chopra

Mishra

Baig

et al. 2021

JoF

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Mushrooms have remained an eternal part of traditional cuisines due to their beneficial health potential and have long been recognized as a folk medicine for their broad spectrum of nutraceuticals, as well as therapeutic and prophylactic uses. Nowadays, they have been extensively investigated to explain the chemical nature and mechanisms of action of their biomedicine and nutraceuticals capacity. Mushrooms belong to the astounding dominion of Fungi and are known as a macrofungus. Significant health benefits of mushrooms, including antiviral, antibacterial, anti-parasitic, antifungal, wound healing, anticancer, immunomodulating, antioxidant, radical scavenging, detoxification, hepatoprotective cardiovascular, anti-hypercholesterolemia, and anti-diabetic effects, etc., have been reported around the globe and have attracted significant interests of its further exploration in commercial sectors. They can function as functional foods, help in the treatment and therapeutic interventions of sub-optimal health states, and prevent some consequences of life-threatening diseases. Mushrooms mainly contained low and high molecular weight polysaccharides, fatty acids, lectins, and glucans responsible for their therapeutic action. Due to the large varieties of mushrooms present, it becomes challenging to identify chemical components present in them and their beneficial action. This article highlights such therapeutic activities with their active ingredients for mushrooms.

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Bioactive Compounds From Microalgae: Current Development and Prospects

Nelson

et al. 2017

Studies in Natural Products Chemistry

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Assessing the immunomodulatory potential of high‐molecular‐weight extracts from mushrooms; an assay based on THP‐1 macrophages

Abstract: An assay was developed suitable to distinguish the immunomodulatory properties of food compounds in a reproducible manner. It was evaluated using eight mushroom species by measuring the secretion of relevant cytokines TNF-α, IL-1β, IL-6 and IL-10.

Cited by 7 publications

References 35 publications

Phytochemical study and immunomodulatory activity of Fraxinus excelsior L.

Phytochemical study and immunomodulatory activity of Fraxinus excelsior L.

Narrative Review: Bioactive Potential of Various Mushrooms as the Treasure of Versatile Therapeutic Natural Product

Bioactive Compounds From Microalgae: Current Development and Prospects

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