Assessing the importance of the root mean square (RMS) value of different waveforms to determine the strength of a dielectrophoresis trapping force

Gilmore, Jordon; Islam, Monsur; Duncan, Josie L.; Natu, Rucha; Martínez-Duarte, Rodrigo

doi:10.1002/elps.201600551

Cited by 18 publications

(13 citation statements)

References 16 publications

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“…Our results in part support the data, i.e., during the PEF + DEP protocols the highest transfection efficiency was reached when (1 MHz) the DEP3 sequence was used. Considering the available knowledge [29,31], the application of square waves, low conductivity buffer, and DEP frequencies including MHz pulsing allows maximizing the DEP force. Such an approach increased the number of transfected cells 1.4-fold (when compared to PEF only), which is competitive even against the HV/LV methodology [14,33].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, Contreras-Dávila et al have shown that the application of square wave more than doubles the DEP entrapment capability compared to the sine [28]. As a result, the lower peak-to-peak voltage can be used to trigger equivalent effects [28,29]. It is beneficial in the electrotransfection context, since the high electric field region can be reduced near the center electrode to prevent electroporation.…”

Section: Introductionmentioning

confidence: 99%

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Dielectrophoretic Manipulation of Cell Transfection Efficiency during Electroporation Using a Center Needle Electrode

et al. 2021

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Long duration electric pulses are frequently used to facilitate DNA electrotransfer into cells and tissues, while electroporation pulses can be combined with electrophoresis to maximize the transfection efficiency. In this work, we present the dielectrophoresis (DEP)-assisted methodology for electrotransfer of plasmid DNA (3.5 kbp pmaxGFP) into mammalian cells (CHO-K1). A prototype of an electroporation cuvette with center needle electrode for DEP-assisted transfection is presented resulting in a 1.4-fold of transfection efficiency increase compared to the electroporation-only procedure (1.4 kV/cm × 100 µs × 8). The efficiency of transfection has been compared between three DEP frequencies of 1, 100, and 1 MHz. Lastly, the effects of exposure time (1, 3, and 5 min) during the DEP application step have been determined. It is concluded that the proposed methodology and exposure setup allow a significant improvement of transfection efficiency and could be used as an alternative to the currently popular electrotransfection techniques.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Manipulation of Cell Transfection Efficiency during Electroporation Using a Center Needle Electrode

et al. 2021

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“…The microfluidic DEP device used in this study featured 3D carbon microelectrode arrays. The fabrication of the carbon microelectrodes has been reported several times in our previous work [8][9][10][11][12]31,[34][35][36][37]. Briefly, the fabrication process included two-step photolithography of SU-8 (Gersteltec, Switzerland), a negative tone photoresist, on a Si/SiO 2 substrate.…”

Section: Device Fabricationmentioning

confidence: 99%

“…Dielectrophoresis (DEP) is a technique that offers the potential for sorting different Candida species in a label-free fashion towards a rapid and affordable assay. DEP is a relatively simple procedure that works by exploiting the specific response of different cells to an electric field gradient [8][9][10][11][12], and has been used for the manipulation, separation, and enrichment of many bioparticles that include bacteria and other bloodborne pathogens [13][14][15][16][17][18][19][20] including Candida albicans [21][22][23][24][25][26][27]. The fact that DEP has been demonstrated in the sorting of cells featuring minor observable differences between them [9,[28][29][30] encourages the study of DEP to isolate specific Candida strains.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes

et al. 2020

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Bloodstream infection with Candida fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to Candida albicans, the rising incidence of infections caused by other Candida strains that may not respond to traditional anti-fungal treatments merits the development of a method for species-specific isolation of Candida. To this end, here we present the characterization of the dielectrophoresis (DEP) response of Candida albicans, Candida tropicalis and Candida parapsilosis. We complement such characterization with a study of the Candida cells morphology. The Candida strains exhibited subtle differences in their morphology and dimensions. All the Candida strains exhibited positive DEP in the range 10–500 kHz, although the strength of the DEP response was different for each Candida strain at different frequencies. Only Candida tropicalis showed positive DEP at 750 kHz. The current results show potential for manipulation and enrichment of a specific Candida strain at specific DEP conditions towards aiding in the rapid identification of Candida strains to enable the effective and timely treatment of Candida infections.

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“…The root mean square mathematical operation is widely used in electrical engineering [34,35]. For this reason, the computing possibilities of the developed system were also tested in measuring the RMS values of the circuit quantities.…”

Section: True Rms Valuementioning

confidence: 99%

Diagnostic approach in assessment of a ferroresonant circuit

Majka

Klimas

2019

Electr Eng

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This paper presents possibilities offered by a diagnostic system called FeD. The system is completely original; it has been developed by the authors on the basis of Arduino platform. The system has been designed to perform and record measurements and to carry out different numerical operations. The real-time function for several operations is incorporated in this system. The necessary input data for the system consist of the electrical voltage waveforms only. Rescaled voltage quantities can be displayed, measured, recorded or computed in any chosen way. The system has been developed particularly for measurements and computations in the ferroresonant circuits. The strongest part of the system is its versatility. It works with a standard PC and supports a universal connection (USB standard). This is undeniably a cost-wise solution. Driving and control of the system functions are carried out using the authors' original software implemented in SciLab environment. This is free software, similar to and compatible with other existing CAD programs such as Octave and MATLAB. The obtained data, scripts and results can be freely transferred between them. The program is equipped with a transparent GUI. The need of constructing a special system to diagnose the ferroresonant circuit has emerged during earlier ferroresonance analyses and computations. Every ferroresonant circuit requires specific kind of diagnostics to estimate and display its base features in order to determine the best scientific approach to the problem. The ferroresonance phenomenon belongs to the domain of nonlinear problems. Its analysis requires excellent skills in mathematics and physics as well as computer science. Moreover, this subject also requires specialized engineering knowledge, particularly in the field of power engineering and power system equipment. Modern mathematical models and analyses used in ferroresonant computations are quite accurate; however, in case of a common user, they are often difficult to understand or implement. This paper provides full description of construction, features and test results of the developed hardware/software system designed for diagnostics of ferroresonant circuits. The test circuit case study has been performed in the entire power supply range. Results of measurements and computations as well as screenshots captured from authors' original software are shown in different figures. The developed software and recorded data have been finally used in modeling and further simulations. During this, the application of the fractional derivative iron core coil model to ferroresonance analysis has been shown. The waveforms obtained from computer simulations have been compared with those obtained from measurements performed in the test circuit.

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Assessing the importance of the root mean square (RMS) value of different waveforms to determine the strength of a dielectrophoresis trapping force

Cited by 18 publications

References 16 publications

Dielectrophoretic Manipulation of Cell Transfection Efficiency during Electroporation Using a Center Needle Electrode

Dielectrophoretic Manipulation of Cell Transfection Efficiency during Electroporation Using a Center Needle Electrode

Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes

Diagnostic approach in assessment of a ferroresonant circuit

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