Assessing the microbial activity of soil samples, its nutrient limitation and toxic effects of contaminants using a simple respiration test

Hollender, Juliane; Althoff, Katrin; Mundt, Matthias; Dott, Wolfgang

doi:10.1016/s0045-6535(03)00556-3

Cited by 50 publications

(15 citation statements)

References 17 publications

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“…Therefore, the addition of inorganic or organic nitrogen-rich nutrients (biostimulation) is an effective approach to enhance the bioremediation process [13–15]. Positive effects of nitrogen amendment on microbial activity and/or petroleum hydrocarbon degradation have been widely demonstrated by various authors [16–19].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments

Abioye

Agamuthu

Aziz

2012

Biotechnology Research International

116

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Soil and surface water contamination by used lubricating oil is a common occurrence in most developing countries. This has been shown to have harmful effects on the environment and human beings at large. Bioremediation can be an alternative green technology for remediation of such hydrocarbon-contaminated soil. Bioremediation of soil contaminated with 5% and 15% (w/w) used lubricating oil and amended with 10% brewery spent grain (BSG), banana skin (BS), and spent mushroom compost (SMC) was studied for a period of 84 days, under laboratory condition. At the end of 84 days, the highest percentage of oil biodegradation (92%) was recorded in soil contaminated with 5% used lubricating oil and amended with BSG, while only 55% of oil biodegradation was recorded in soil contaminated with 15% used lubricating oil and amended with BSG. Results of first-order kinetic model to determine the rate of biodegradation of used lubricating oil revealed that soil amended with BSG recorded the highest rate of oil biodegradation (0.4361 day−1) in 5% oil pollution, while BS amended soil recorded the highest rate of oil biodegradation (0.0556 day−1) in 15% oil pollution. The results of this study demonstrated the potential of BSG as a good substrate for enhanced remediation of hydrocarbon contaminated soil at low pollution concentration.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments

Abioye

Agamuthu

Aziz

2012

Biotechnology Research International

116

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“…Various factors may limit the rate of petroleum hydrocarbon degradation including lack of essential nutrients such as nitrogen. Therefore, the addition of inorganic or organic nitrogen-rich nutrients (biostimulation) is an effective approach to enhance the bioremediation process (Hollender et al 2003;Semple et al 2006;Walworth et al 2007). Positive effects of nitrogen amendment on microbial activity and/or petroleum hydrocarbon degradation have been widely demonstrated (Jørgensen et al 2000;Margesin et al 2000Margesin et al , 2007Brook et al 2001;Margesin and Schinner 2001;Riffaldi et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biodegradation of Used Engine Oil in Soil Amended with Organic Wastes

Abioye

Aziz

Agamuthu

2009

Water Air Soil Pollut

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Three organic wastes (banana skin (BS), brewery spent grain (BSG), and spent mushroom compost (SMC)) were used for bioremediation of soil spiked with used engine oil to determine the potential of these organic wastes in enhancing biodegradation of used oil in soil. The rates of biodegradation of the oil were studied for a period of 84 days under laboratory conditions. Hydrocarbon-utilizing bacterial counts were high in all the organic waste-amended soil ranging between 10.2×10 6 and 80.5×10 6 CFU/g compared to unamended control soil throughout the 84 days of study. Oil-contaminated soil amended with BSG showed the highest reduction in total petroleum hydrocarbon with net loss of 26.76% in 84 days compared to other treatments. First-order kinetic model revealed that BSG was the best of the three organic wastes used with biodegradation rate constant of 0.3163 day −1 and half-life of 2.19 days. The results obtained demonstrated the potential of organic wastes for oil bioremediation in the order BSG>BS>SMC.

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“…The extended lag phase observed in the production of CO 2 on the uninoculated treatment may indicate a toxic effect of gasoline on the native soil organisms24, 25 or even at a low initial density. In the Brazilian commercial gasoline, both monoaromatic hydrocarbons benzene, toluene, xylene, and ethanol (BTEX) contribute to the toxicity of the fuel.…”

Section: Discussionmentioning

confidence: 99%

Changes in the microbial community during bioremediation of gasoline-contaminated soil

Leal

Rodrigues

Leal

et al. 2017

Brazilian Journal of Microbiology

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We aimed to verify the changes in the microbial community during bioremediation of gasoline-contaminated soil. Microbial inoculants were produced from successive additions of gasoline to municipal solid waste compost (MSWC) previously fertilized with nitrogen-phosphorous. To obtain Inoculant A, fertilized MSWC was amended with gasoline every 3 days during 18 days. Inoculant B received the same application, but at every 6 days. Inoculant C included MSWC fertilized with N–P, but no gasoline. The inoculants were applied to gasoline-contaminated soil at 10, 30, or 50 g/kg. Mineralization of gasoline hydrocarbons in soil was evaluated by respirometric analysis. The viability of the inoculants was evaluated after 103 days of storage under refrigeration or room temperature. The relative proportions of microbial groups in the inoculants and soil were evaluated by FAME. The dose of 50 g/kg of inoculants A and B led to the largest CO2 emission from soil. CO2 emissions in treatments with inoculant C were inversely proportional to the dose of inoculant. Heterotrophic bacterial counts were greater in soil treated with inoculants A and B. The application of inoculants decreased the proportion of actinobacteria and increased of Gram-negative bacteria. Decline in the density of heterotrophic bacteria in inoculants occurred after storage. This reduction was bigger in inoculants stored at room temperature. The application of stored inoculants in gasoline-contaminated soil resulted in a CO2 emission twice bigger than that observed in uninoculated soil. We concluded that MSWC is an effective material for the production of microbial inoculants for the bioremediation of gasoline-contaminated soil.

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Assessing the microbial activity of soil samples, its nutrient limitation and toxic effects of contaminants using a simple respiration test

Cited by 50 publications

References 17 publications

Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments

Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments

Enhanced Biodegradation of Used Engine Oil in Soil Amended with Organic Wastes

Changes in the microbial community during bioremediation of gasoline-contaminated soil

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