Assessing the Penetrating Abilities of Experimental Preparation with Dental Infiltrant Features Using Optical Microscope: Preliminary Study

Skucha-Nowak, Małgorzata; Machorowska-Pieniążęk, Agnieszka; Tanasiewicz, Marta

doi:10.17219/acem/63007

Cited by 13 publications

(27 citation statements)

References 7 publications

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“…Icon (DMG, Hamburg, Germany) is an example of a commercially available dental infiltrant preparation, which was launched in 2009. It is intended for treatment of carious lesions located on smooth and tangential tooth surfaces [9][10][11][12][13]. Icon is recommended for use in early-stage carious lesions to a maximum depth of D1 (Manji radiographic classification of lesion depth) [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Icon is recommended for use in early-stage carious lesions to a maximum depth of D1 (Manji radiographic classification of lesion depth) [13,14]. In order to be referred to as a dental infiltrant, the preparation should feature low density, high surface tension, hydrophilicity, no toxic effects on the organism, bacteriostaticity, no interaction with food and drugs, ability to polymerise to a solid state, resistance to chemical and mechanical agents and have good aesthetics after polymerisation [9]. The advantages of the Icon commercial preparation include the ability to polymerise to a solid state, resistance to chemical and mechanical agents and its ability to mimic the tooth colour.…”

Section: Introductionmentioning

confidence: 99%

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Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

et al. 2021

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Microinvasive dentistry is based on the treatment of early carious lesions with the use of dental infiltrants. The commercially available Icon dental infiltrant does not contain any bacteriostatic component. An experimental preparation enriched with the missing component was synthesised. The aim of this study was to evaluate the cytotoxicity of the experimental preparation. Mouse fibroblasts of the L-929 lineage were used for the in vitro study. Cell morphology and viability were assessed. In the cytotoxicity analysis, it was shown that the experimental preparation (42.8 ± 10.3) after 24 h at two-fold dilution showed similar cytotoxicity to Icon (42.7 ± 8.8) (p > 0.05), while at four-fold dilution experimental preparation (46.7 ± 3.1), it was less toxic than Icon (34.2 ± 3.1) (p < 0.05). The experimental preparation has the potential to provide an alternative to the Icon commercial preparation. Further research is needed to evaluate the cytotoxicity of the experimental preparation over a longer period of time.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

et al. 2021

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“…The difference in the values of the penetration coefficient between our own experimental preparation synthesized for the purpose of the study in comparison to the commercially available Icon preparation is small, which was noticed by M. Skucha-Nowak et al [13].…”

Section: Discussionmentioning

confidence: 68%

“…In the case of the tested teeth, the presence of YbF3 grains was found both on the tooth surface and in deeper layers. Therefore, it is difficult to determine the method of infiltration [13,26]. On the other hand, the penetration depth values obtained for both preparations are very low as the depth of etched enamel canals with Icon-Etch is 30-40 µm and the dental infiltrant should fully seal them to block the access for carious bacteria and the products of their metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…After extraction, the teeth were stored in a chloramine solution. Before the onset of the study, the chloramine solution was removed, and the teeth were rinsed very thoroughly in distilled water and left in it for 24 h. After rinsing and drying, the teeth were chemically decalcified for 4 weeks in an incubator at 37 • C [13]. After the decalcification process was completed, the teeth were rinsed three times in distilled water and left in it for 24 h. Finally, the teeth were dried.…”

Section: Methodsmentioning

confidence: 99%

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Use of Ytterbium Trifluoride in the Field of Microinvasive Dentistry—An In Vitro Preliminary Study

et al. 2020

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Background: The aim of this study was an attempt at determining the quantity of the degree of the addition of ytterbium trifluoride as a marker, aimed at facilitating the observation and assessment of the effectiveness of penetration into the decalcified enamel of human teeth of an experimental preparation with the characteristics of a dental infiltrant and a commercially available commercial preparation called Icon. Methods: The test material was 20 decalcified human teeth. The first half of the batch was soaked in Icon, the second half in an experimental preparation with the characteristics of a dental infiltrant and with a component responsible for bacteriostaticity. Ytterbium trifluoride was added to both preparations to facilitate the microscopic observations: 20 mg/1 g in the first phase of the experiment, 60 mg/1 g in the second phase. Results: YbF3 particles could not be found in the teeth from the first phase of the experiment. Particles rich in ytterbium could be found only in the teeth from the second phase of the experiment, with three times the content of ytterbium. Conclusion: The addition of 6% ytterbium trifluoride (both commercial and laboratory synthesized) facilitates microscopic observation, allowing the conclusion that both Icon and the experimental preparation with the characteristics of a dental infiltrant penetrate the decalcified enamel of a human tooth. The SEM analysis of the preparations in terms of content and particle size of ytterbium trifluoride shows that the distribution is heterogeneous. Large size particles predominate, yet particles with a diameter of less than 1 µm were also found. This may confirm the fact that most of them have probably agglomerated. The method of scattering YbF3 nanoparticles in the infiltrant resin requires further work so that they do not appear as agglomerates.

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The combined approach to evaluation of mechanical behavior of tooth enamel in artificially induced caries lesions

et al. 2020

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Improved knowledge of caries initiation and progression on the base of modern mechanobiology could facilitate effective preventing and therapeutic strategies. Not all of previously developed experimental models of artificial caries comply with required standards. The aim of the study was to investigate microstructural and mechanical properties of 12 artificial caries lesions, induced on human intact premolars according to original protocol. The tooth specimens were subjected to imaging by X-ray CT system Nikon Metrology XT H 225, with further morphometric analysis of lesion geometry, depth, surface layer thickness and mineral density in the areas of interest. Mechanical characteristics (penetration depth, microhardness, elastic modulus) in enamel lesion, perifocal intact enamel and remote zones were obtained using Micro CombiTester system. Clinically all lesions fully corresponded to ICDAS – 2 code. According to µCT data, LDµCT of the lesions ranged from 346.4 to 531 µm, mineral density range from 2.03±0.15 g/cm3 in transparent zone to 1.05±0.08 g/cm3 in the surface zone of the lesion. Microhardness of demineralized enamel lesion more than 5 times reduced in comparison with perifocal and remote intact enamel. Nanoindentation and µCT data are corresponds to those presenting on the base of other classical protocols of artificial caries formation and close to the typical mechanical characteristics of enamel caries in vivo.

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Assessing the Penetrating Abilities of Experimental Preparation with Dental Infiltrant Features Using Optical Microscope: Preliminary Study

Cited by 13 publications

References 7 publications

Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

Use of Ytterbium Trifluoride in the Field of Microinvasive Dentistry—An In Vitro Preliminary Study

The combined approach to evaluation of mechanical behavior of tooth enamel in artificially induced caries lesions

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