Assessing the Performance of a Bdelloid Rotifer Philodina acuticornis odiosa Acute Toxicity Assay

Hagen, Tarah; Allinson, Graeme; Wightwick, Adam; Nugegoda, Dayanthi

doi:10.1007/s00128-008-9611-6

Cited by 14 publications

(7 citation statements)

References 14 publications

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“…Taking into account that a toxicological evaluation in aquatic ecosystems requires a set of species representative of a variety of ecological niches, the use of P. roseola, a benthic species will be relevant since most used rotifer species belong to the plankton, as mentioned before. Also, species of the genus Philodina fullfi l the biological characteristics recommended for a test organism (Buikema et al 1974, Hagen et al 2009, Allinson et al 2011), besides occurring in a variety of freshwater environments (Wallace andSnell 2010, Sharma andSharma 2012). In freshwater reservoirs of Southern Brazil, for example they are frequent, developing large populations (SouzaSoares et al 2011, Garraffoni andLourenço 2012).…”

Section: Discussionmentioning

confidence: 99%

“…To prevent evaporation of the culture medium, the beakers were sealed with plastic fi lm. Every 76 hours, as proposed by Hagen et al (2009), the water and food were renewed. The rotifers were fed on a suspension of the alga Raphidocelis subcapitata, grown in CHU-12 medium (Müller 1972), at a density of 1x10 5 cells mL -1 .…”

Section: Maintenance Of Rotifer Stock Culturementioning

confidence: 99%

“…Species of the genus Philodina have biological characteristics that immediately recommend them as environmental test organisms (Buikema et al 1974, Hagen et al 2009, Allinson et al 2011. Adult individuals are small, they reproduce only by parthenogenesis, have high fecundity and the life-cycle is short.…”

Section: Introductionmentioning

confidence: 99%

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Life cycle traits of Philodina roseola Ehrenberg, 1830 (Rotifera, Bdelloidea), a model organism for bioassays

Moreira

Mansano

2016

An. Acad. Bras. Ciênc.

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This paper describes experimental results on the life cycle of the rotifer Philodina roseola cultured in the laboratory. Detailed information on life-cycle parameters of a certain species provides a deep understanding and contributes to a better knowledge of the role of the species in the community, besides providing data that are basic to other ecological investigations such as secondary production estimates and knowledge for applications such as its utilization as test-organism in ecotoxicological studies. The average duration of embryonic development of P. roseola was 23.88 h, the age at maturity of primipara was 3.5 days and the maximum lifespan was 23 days. The average size of the rotifer neonate was 198.77 μm, the mean size of primipara was 395.56 μm and for adults 429.96 μm. The average fecundity was 1.22 eggs per female per day and the mean number of eggs produced per female during the entire life was 22.33. The deceleration of somatic growth from the start of the reproductive stage represents a trade-off between growth and reproduction that is often seen in micrometazoans. The life history of P. roseola follows the strategy of other bdelloid species characterized by a rapid pre-reproductive development and canalization of most assimilated energy to reproduction after reaching maturity. The differences observed in total fecundity and longevity between our P. roseola cultures and those from previous studies were probably due to differences of intrinsic adaptation of this species ecotypes to the conditions of their natural environments.

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Section: Discussionmentioning

confidence: 99%

Section: Maintenance Of Rotifer Stock Culturementioning

confidence: 99%

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Life cycle traits of Philodina roseola Ehrenberg, 1830 (Rotifera, Bdelloidea), a model organism for bioassays

Moreira

Mansano

2016

An. Acad. Bras. Ciênc.

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“…Studies on acute water toxicity assays are known for the species Philodina acuticornis odiosa (Hagen et al, 2009), Brachionus havanaensis (Juárez-Franco et al, 2007), Brachionus macracanthus (Nandini et al, 2007), Brachionus patulus (Sarma et al, 2006) Brachionus calyciflorus (Calleja et al, 1994), Brachionus plicatilis (Snell et al, 1991), Brachionus rubens (Halbach et al, 1983); however, there are no records of tests with rotifers in Brazil.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Atibaia River water quality using Lecane bulla as a test organism

et al. 2015

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In view of the diversity of environments found in the Brazilian territory, it is understandable that the use of native species can provide more relevant information for ecotoxicological studies. The purpose of this work was to evaluate the quality of water samples from the Atibaia River in an area that is under the influence of petroleum refinery using a native test-organism and submitting the data to PCA statistical analysis. Therefore, acute toxicity assays with Lecane bulla (Rotifera) were performed in four locations of the river, as well as physical-chemical analyses. Sampling was drawn in the dry and rainy seasons. The bioassays were static and lasted 48 hours; dead organisms were quantified at the end of the tests. Toxicological differences among the samples/per location and control were compared by means of the Analysis of Variance. Physical-chemical and mortality variables were simultaneously analyzed by multivariate analysis of the principal components and the Pearson correlation coefficient. Water samples from the exit of the refinery stabilization pond (location S.1) were toxic to L. bulla in both seasons, with significant differences in relation to the control and between the seasons. The statistical treatment of data showed that mortality was strong and positively correlated with total hardness, chlorides and EC, which together with pH presented higher values in location S.1, in the dry and in the rainy seasons. Due to its sensibility to the quality of the Atibaia river water samples, the potential use of L. bulla for ecotoxicological studies as an alternative test organism could be demonstrated.

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“…UV light, temperature, toxins) in their diapause stage. Only bdelloid rotifers have an uncommon degenerate tetraploid genome consisting of numerous genes acquired by horizontal gene transfer (Hagen et al, 2009). The reproduction of bdelloid rotifers is also obligatorily asexual, and males have been extinct for more than 30 million years.…”

Section: Introductionmentioning

confidence: 99%

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

Oláh

Bush

Aleksza

et al. 2017

Ecotoxicology and Environmental Safety

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Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (HO) and sodium azide (NaN) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science.

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Assessing the Performance of a Bdelloid Rotifer Philodina acuticornis odiosa Acute Toxicity Assay

Cited by 14 publications

References 14 publications

Life cycle traits of Philodina roseola Ehrenberg, 1830 (Rotifera, Bdelloidea), a model organism for bioassays

Life cycle traits of Philodina roseola Ehrenberg, 1830 (Rotifera, Bdelloidea), a model organism for bioassays

Evaluation of the Atibaia River water quality using Lecane bulla as a test organism

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

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