The critical thinking style that a person has will determine the extent of critical thinking skills. This study aims to look at differences in the critical thinking styles of biology teacher candidates based on academic level. The method applied in this study is non-experimental with a comparative design. The instrument used was the Yanpiaw Creative-Critical Style Test which was given to a number of prospective biology teacher students from semester 2, semester 4, semester 6 and semester 8 as respondents. The results showed that there was a significant difference between the average critical thinking style between students in semester 2 and semester 6 (sig. 0.011 < 0.05) and between semester 2 and semester 8 (sig. 0.029 < 0.05). Based on the findings, it can be concluded that academic level or level does not always affect a person's critical thinking style, but it is also possible due to academic majors, behavior style, ethnicity (culture). Therefore, it is important for an educator to provide learning methods and media that are able to support the development of students' critical thinking styles