Assessing the Viability of Bacterial Species in Drinking Water by Combined Cellular and Molecular Analyses

Kahlisch, Leila; Henne, Karsten; Gröbe, Lothar; Brettar, Ingrid; Höfle, Manfred G.

doi:10.1007/s00248-011-9918-4

Cited by 19 publications

(22 citation statements)

References 36 publications

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“…The taxonomic analyses of the bulk water samples of this study complement the analyses of two former studies of the same DWDS (11,21). This allows us to extend the observation period of the bulk water in contact with the mature biofilms by two more sampling periods in 2003 (11) and 2008 (21), thus covering at least partially a period of a total of 6 years, i.e., from 2003 to 2009.…”

Section: General Properties Of Bulk Water and Biofilmmentioning

confidence: 54%

“…S3 in the supplemental material). Compared to the former studies of this DWDS (11,21), only a phylotype related to Nitrospira moscoviensis was redetected at a low abundance by DNA analyses of biofilm samples (PT B018; 100% 16S rRNA similarity to a PT retrieved from RNA of bulk water in 2003) ( Fig. 4B; also see Table S4 in the supplemental material).…”

Section: General Properties Of Bulk Water and Biofilmmentioning

confidence: 98%

“…This allows us to extend the observation period of the bulk water in contact with the mature biofilms by two more sampling periods in 2003 (11) and 2008 (21), thus covering at least partially a period of a total of 6 years, i.e., from 2003 to 2009. For the bulk water, the current study shows a phylum composition similar to that found in the two former studies; concerning phylotype reoccurrence, 34% of the phylotypes of this study could be considered identical to those of former studies when a distinction of 0 to 3 nucleotides (nt) (corresponding to a 16S rRNA similarity of 99% or higher) is applied, and we found a reoccurrence of 41% based on a 4-to 6-nt distinction (98% similarity) (see Table S3 in the supplemental material).…”

Section: General Properties Of Bulk Water and Biofilmmentioning

confidence: 99%

“…To this end, mature biofilm was sampled from different pipe materials at different locations in a small-scale network in continuous use for 20 years to assess spatial heterogeneity (11,17,21). By comparing DNA-and RNAbased fingerprints, we wanted to test the hypothesis that biofilms show the same complete differences between these two types of fingerprints as the bulk water (11).…”

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confidence: 99%

“…On the other hand, the 16S rRNA concentration is dependent on the ribosome content of the bacterial cell, which rises with increasing growth rate or activity, and is currently understood to be an indicator of bacterial activity (35). Therefore, the use of RNA-based fingerprints could help to screen for active phylotypes and/or to detect low-abundance but active phylotypes that are not detected by DNA-based techniques (11,21,23,27).…”

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confidence: 99%

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Analysis of Structure and Composition of Bacterial Core Communities in Mature Drinking Water Biofilms and Bulk Water of a Citywide Network in Germany

Henne

Kahlisch

Brettar

et al. 2012

Appl Environ Microbiol

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137

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ABSTRACTThe bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the city of Braunschweig, Germany, whereas all biofilm samples contained a unique community with no overlapping phylotypes from bulk water. Biofilm samples consisted mainly ofAlphaproteobacteria(26% of all phylotypes),Gammaproteobacteria(11%), candidate division TM6 (11%),Chlamydiales(9%), andBetaproteobacteria(9%). The bulk water community consisted primarily ofBacteroidetes(25%),Betaproteobacteria(20%),Actinobacteria(16%), andAlphaproteobacteria(11%). All biofilm communities showed higher relative abundances of single phylotypes and a reduced richness compared to bulk water. Only biofilm communities sampled at nearby sampling points showed similar communities irrespective of support materials. In all of our bulk water studies, the community composition determined from 16S rRNA was completely different from the 16S rRNA gene-based community composition, whereas in biofilms both molecular fractions resulted in community compositions that were similar to each other. We hypothesize that a higher fraction of active bacterial phylotypes and a better protection from oxidative stress in drinking water biofilms are responsible for this higher similarity.

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Section: General Properties Of Bulk Water and Biofilmmentioning

confidence: 54%

Section: General Properties Of Bulk Water and Biofilmmentioning

confidence: 98%

Section: General Properties Of Bulk Water and Biofilmmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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