Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic Patients

Abstract

Aim of the work:To identify the relationship between serum concentration of 7,8-dihydro-2-deoxyguanosine (8-OHdG) and different stages of Albumin Excretion Rate (AER), as marker of nephropathy in Egyptian patients with Type 1 Diabetes(T1D). Subjects and methodology:Total 65 volunteers were distributed in 4 groups: group 1 included 20 healthy subjects as control, group 2: 15 T1D patients with AER<30 mg/g creatinine, group 3:15 T1D patients with (300<AER> 30 mg/g creatinine) and group 4:15 T1D patients with (AER>30 mg/g creatinine). The blood chemistry parameters were analyzed, glycated hemoglobin (HbA1c) and serum urea and creatinine, Malodialdhyde (MDA), 8-oxodG levels were measured by ELISA.Results: Significant increase in urea and creatinine in G3 and G4 with control with a p value<0.005. Also increase in 8-OHdG and MDA with a p value<0.005 in G2, G3 and G4 compared with control.