Assessment of a photobioreactor-coupled modified Robbins device to compare the adhesion of Nannochloropsis gaditana on different materials

Zeriouh, Ouassim; Reinoso-Moreno, J.V.; López-Rosales, L.; Cerón-García, M.C.; Sánchez-Mirón, A.; Camacho, F. Garcı́a; Grima, E. Molina

doi:10.1016/j.algal.2018.12.008

Cited by 10 publications

(13 citation statements)

References 28 publications

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“…A glass bench‐scale flat‐panel photobioreactor (FP‐PBR) was used for the indoor experiments. The operational details and the dimensions of the FP‐PBR have been described elsewhere (Zeriouh et al, ). The FP‐PBR has a 13 L volume; it is illuminated with artificial light 12 hr/24 hr and operated in continuous mode at a dilution rate of 0.3 per day.…”

Section: Methodsmentioning

confidence: 99%

“…The cell status of the cultures was checked daily by measuring the maximum photochemical yield of the photosystem II (PSII), F v / F m , ratio with a fluorometer (Mini‐PAM‐2500, Heinz Walz, Effeltrich, Germany). The nitrate and phosphate concentrations dissolved in the supernatant and the biomass concentration in the cultures were determined spectrophotometrically as described earlier (Zeriouh et al, ; Zeriouh, Reinoso‐Moreno, López‐Rosales, Sierra‐Martín, et al, ). Additionally, flow cytometry was used to quantify the cultures’ cell density (cells per ml).…”

Section: Methodsmentioning

confidence: 99%

“…For the indoor experiments, six rigid materials with smooth surfaces were used (Zeriouh et al, ): polyvinylchloride (PVC), polycarbonate (PC), polystyrene (PS), borosilicate glass (GL), stainless steel (SS), and polyethylene (PE). These materials were supplied in the form of 7.88‐mm diameter disk coupons (Tyler Research Corp, Edmonton, Canada) compatible with the Modified Robbins Device (MRD) flow channel (LPMR‐12PMMA; Tyler Research Corp).…”

Section: Methodsmentioning

confidence: 99%

“…Before measuring the contact angles, the coatings and rigid materials used in outdoor pilot‐plant experiments were washed with Alconox detergent (1%), then rinsed with abundant deionized water and allowed to dry at room temperature. Meanwhile, the surfaces prepared for the indoor experiments were washed according to the protocol previously described (Zeriouh et al, ). Briefly, the disc coupon materials mounted in the MRD were rinsed out by closed‐circuit recirculating in hot water (40°C) containing 1% Alconox ® at a shear rate of 300 s −1 .…”

Section: Methodsmentioning

confidence: 99%

“…Understanding the underlying phenomenology and fundamentals is essential for proposing solutions. Methodologies for determining the physicochemical properties of both the microalgae surface and the rigid materials that are potentially useful for fabricating PBRs used in the cultivation of planktonic marine microalgae have previously been developed (Zeriouh et al, ; Zeriouh, Reinoso‐Moreno, López‐Rosales, Cerón‐García, et al, ; Zeriouh, Reinoso‐Moreno, López‐Rosales, Sierra‐Martín, et al, ). In this context, the biocompatibility theories developed by Baier and Vogler (Baier, , ; Vogler, , , ) may also help to design a universal antibiofouling surface for PBRs.…”

Section: Introductionmentioning

confidence: 99%

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New insights into developing antibiofouling surfaces for industrial photobioreactors

Zeriouh

Marco‐Rocamora

Reinoso-Moreno

et al. 2019

Biotech & Bioengineering

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The biofouling formation of the marine microalga Nannochloropsis gaditana on nontoxic surfaces was quantified on rigid materials, both coated (with fouling release coatings and nanoparticle coatings) and noncoated, to cover a wide range of surface properties from strongly hydrophobic to markedly hydrophilic under conditions similar to those prevailing in outdoor massive cultures of marine microalgae. The effect of seawater on surfaces that presented the best antibiofouling properties was also evaluated. The adhesion intensity on the different surfaces was compared with the predictions of the biocompatibility theories developed by Baier and Vogler using water adhesion tension (τ 0 ) as the quantitative parameter of surface wettability. For the most hydrophobic surfaces, τ 0 ≤ 0, the microalgae adhesion density increased linearly with τ 0 , following the Baier's theory trend. However, for the rest of the surfaces, τ 0 ≥ 0, a tendency toward minimum adhesion was observed for amphiphilic surfaces with a τ 0 = 36 mJ/m 2 , a value close to that which minimizes cell adhesion according to Vogler's theory. The understanding and combination of the two biocompatibility theories could help to design universal antibiofouling surfaces that minimize the van der Waals forces and prevent foulant adsorption by using a thin layer of hydration. K E Y W O R D Santibiofouling, biocompability theory, fouling release coatings, microalga, photobioreactor

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

New insights into developing antibiofouling surfaces for industrial photobioreactors

Zeriouh

Marco‐Rocamora

Reinoso-Moreno

et al. 2019

Biotech & Bioengineering

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Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Soriano-Jerez

López-Rosales

Cerón-García

et al. 2020

Biotech & Bioengineering

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Biofouling represents an important limitation in photobioreactor cultures. The biofouling propensity of different materials (polystyrene, borosilicate glass, polymethyl methacrylate, and polyethylene terephthalate glycol‐modified) and coatings (two spray‐applied and nanoparticle‐based superhydrophobic coatings and a hydrogel‐based fouling release coating) was evaluated by means of a short‐term protein test, using bovine serum albumin (BSA) as a model protein, and by the long‐term culture of the marine microalga Nannochloropsis gaditana under practical conditions. The results from both methods were similar, confirming that the BSA test predicts microalgal biofouling on surfaces exposed to microalgae cultures whose cells secrete macromolecules, such as proteins, with a high capacity for forming a conditioning film before cell adhesion. The hydrogel‐based coating showed significantly reduced BSA and N. gaditana adhesion, whereas the other surfaces failed to control biofouling. Microalgal biofouling was associated with an increased concentration of sticky extracellular proteins at low N/P ratios (below 15).

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Substrate properties as controlling parameters in attached algal cultivation

Karimi

Laughinghouse

Davis

et al. 2021

Appl Microbiol Biotechnol

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Assessment of a photobioreactor-coupled modified Robbins device to compare the adhesion of Nannochloropsis gaditana on different materials

Cited by 10 publications

References 28 publications

New insights into developing antibiofouling surfaces for industrial photobioreactors

New insights into developing antibiofouling surfaces for industrial photobioreactors

Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Substrate properties as controlling parameters in attached algal cultivation

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