Assessment of biotin interference with qualitative point-of-care hCG test devices

Williams, Grace R; Cervinski, Mark A.; Nerenz, Robert D.

doi:10.1016/j.clinbiochem.2018.01.018

Cited by 21 publications

(4 citation statements)

References 11 publications

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“…Biotin has become a popular over-the-counter dietary supplement, and it is also being prescribed in mega-doses to a subset of multiple sclerosis patients owing to its beneficial effects on clinical outcomes and quality of life [18], [19]. Biotin-mediated interference has been documented in several immunoassays, leading to misdiagnosis and inappropriate treatment in some instances [20], [21], [22], [23], [24], [25], [26]. All of the AQT90 assays, including the hCG assay, rely on a biotinylated capture antibody that is immobilized on the reaction cup surface with the help of streptavidin [4].…”

Section: Discussionmentioning

confidence: 99%

Analytical evaluation of the Radiometer AQT90 FLEX βhCG assay

Brun

Holloway

Oleksy

et al. 2019

Practical Laboratory Medicine

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ObjectivesMany hospitals cannot afford an hCG assay on a central lab analyzer and turn to point of care testing (POCT) solutions. The Radiometer AQT90 FLEX is a small benchtop immunoareement between the AQT90 and comparator methods for samples with hCG ssay analyzer for use in the laboratory or at the patient bedside. This study evaluated the analytical performance of the AQT90's βhCG assay.MethodsPrecision was assessed using whole blood patient samples and two levels of quality control. Linearity was assessed by dilution of a high hCG plasma sample. Carryover and hook effect were assessed using high and low hCG samples. Method comparisons were done against Abbott i-STAT Total βhCG, Beckman Coulter Total βhCG (5th IS), and Roche hCG+β. Sample concentrations ranged from<2 IU/L to 4,973 IU/L.ResultsRepeatability and within-laboratory precision passed most manufacturer's claims and allowable error criteria. Linearity was validated from<2 IU/L to 4,741 IU/L. Hook effect was not observed up to 2,446,448 IU/L. Carryover was<4.0 ppm. A linear relationship was observed with i-STAT, Beckman and Roche methods. At>20 IU/L, biases were apparent against all three comparator assays (i-STAT: +20%, Roche: +30%, Beckman: +5 to 15%). At ≤20 IU/L, the acceptability of agreement varied according to TAE specifications. Concordance between AQT90 and comparator assays using 5 IU/L as the medical decision level ranged from 69% to 81%.ConclusionsOverall, the AQT90 hCG assay performed well and would be suitable for smaller suburban or rural hospitals. Some limitations have been noted and should be kept in mind during clinical testing.

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Section: Discussionmentioning

confidence: 99%

Analytical evaluation of the Radiometer AQT90 FLEX βhCG assay

Brun

Holloway

Oleksy

et al. 2019

Practical Laboratory Medicine

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“…Therefore, clinicians and laboratory technicians need to be aware of this potential interference with qualitative urine hCG tests and should suggest quantitative serum hCG measurement. The latter is not subject to biotin interference [ 33 ].…”

Section: Vitamin Bmentioning

confidence: 99%

The Role of Vitamins and Minerals in Hair Loss: A Review

Almohanna

Ahmed

Tsatalis

et al. 2018

Dermatol Ther (Heidelb)

203

156

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People commonly inquire about vitamin and mineral supplementation and diet as a means to prevent or manage dermatological diseases and, in particular, hair loss. Answering these queries is frequently challenging, given the enormous and conflicting evidence that exists on this subject. There are several reasons to suspect a role for micronutrients in non-scarring alopecia. Micronutrients are major elements in the normal hair follicle cycle, playing a role in cellular turnover, a frequent occurrence in the matrix cells in the follicle bulb that are rapidly dividing. Management of alopecia is an essential aspect of clinical dermatology given the prevalence of hair loss and its significant impact on patients’ quality of life. The role of nutrition and diet in treating hair loss represents a dynamic and growing area of inquiry. In this review we summarize the role of vitamins and minerals, such as vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, iron, selenium, and zinc, in non-scarring alopecia. A broad literature search of PubMed and Google Scholar was performed in July 2018 to compile published articles that study the relationship between vitamins and minerals, and hair loss. Micronutrients such as vitamins and minerals play an important, but not entirely clear role in normal hair follicle development and immune cell function. Deficiency of such micronutrients may represent a modifiable risk factor associated with the development, prevention, and treatment of alopecia. Given the role of vitamins and minerals in the hair cycle and immune defense mechanism, large double-blind placebo-controlled trials are required to determine the effect of specific micronutrient supplementation on hair growth in those with both micronutrient deficiency and non-scarring alopecia to establish any association between hair loss and such micronutrient deficiency. Plain Language Summary: Plain language summary available for this article.

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“…11 Nail fragility due to mechanical trauma in onychotillomania: the distal margin of the nail has an irregular fragmented appearance with scaling Fig. 12 Nail fragility due to chemical trauma by prolonged use of colored nail lacquer: yellow discoloration and onychorrhexis more marked in the distal nail altered [32][33][34]. The presence of biotin can interfere with tests that use biotin-streptavidin technology.…”

Section: Treatment: Facts and Controversiesmentioning

confidence: 99%

Pathogenesis, Clinical Signs and Treatment Recommendations in Brittle Nails: A Review

Chessa

Iorizzo²,

Richert

et al. 2019

Dermatol Ther (Heidelb)

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Nail plate brittleness (or fragility) is a common complaint affecting up to 20% of the population, especially women over 50 years of age, with fingernail fragility being more prevalent than toenail fragility. Nail brittleness is characterized by nails that split, flake and crumble, become soft and lose elasticity. The main clinical presentations are: onychoschizia, onychorrhexis, superficial granulation of keratin and worn-down nails. According to causative factors, we can distinguish 2 forms of nail fragility (NF): a primary ''idiopathic or brittle nail syndrome'' form and NF secondary to different causes such as inflammatory nail disorders, infections, systemic diseases and general conditions, traumas and alteration of the nail hydration. Optimal management requires treatment of the primary cause of brittle nails, when possible. In idiopathic NF oral supplementation, vitamins (especially biotin, also known as vitamin B7), trace elements and amino acids (especially cysteine) have been reported to be useful. In addition, several products, such as topical moisturizers and lacquers could be considered to restructure the affected nail plate and to reduce psychological impacts of this common problem.

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Assessment of biotin interference with qualitative point-of-care hCG test devices

Cited by 21 publications

References 11 publications

Analytical evaluation of the Radiometer AQT90 FLEX βhCG assay

Analytical evaluation of the Radiometer AQT90 FLEX βhCG assay

The Role of Vitamins and Minerals in Hair Loss: A Review

Pathogenesis, Clinical Signs and Treatment Recommendations in Brittle Nails: A Review

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