Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution

Svitková, Veronika; Blaškovičová, Jana; Tekelova, Monika; Kallai, Brigitta Margit; Ignat, Teodora; Horáčková, Veronika; Skládal, Petr; Kopel, Pavel; Adam, Vojtěch; Farkašová, Dana; Labuda, Ján

doi:10.1016/j.snb.2016.11.160

Cited by 24 publications

(41 citation statements)

References 31 publications

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“…The portions of survived DNA (indicated by the CV evaluation) at the biosensors bearing plasmid and chromosomal (calf thymus) DNA have been compared with those obtained previously with salmon sperm dsDNA for 60 s and 300 s of UV‐C irradiation . In all cases the biosensors construction and procedures were the same.…”

Section: Resultsmentioning

confidence: 99%

“…In our complex study presented here, the ROS generation induced by the UV‐C irradiation of CdS QDs of about 4.0 nm size exhibiting a high fluorescence has been confirmed spectrophotometrically using specific UV‐Vis indicators and chemically using radical scavangers. Voltammetric and impedimetric measurements with the electrochemical biosensors based on DNA modified GCE electrodes confirmed the deep DNA degradation for both the plasmid and chromosomal DNA.…”

Section: Discussionmentioning

confidence: 99%

“…Their 0.5 mg mL −1 stock solutions were prepared in 0.1 M phosphate buffer (PB) of pH 7.4 containing 10 mM KCl. CdS QDs of the size about 4.0 nm stabilized with mercaptopropionic acid were synthetized at Mendel University, Brno, Czech Republic according to the published modification and characterization . TAE (Tris‐acetate‐EDTA) buffer and ethidium bromide were purchased from AppliChem, Germany.…”

Section: Methodsmentioning

confidence: 99%

“…3 nm) with lower fluorescence intensity and isolated salmon sperm DNA was degradated to deeper degree than the calf thymus one. Recently, we have published structural damage to salmon sperm dsDNA at its exposure to UV‐C radiation in the presence of CdS QDs of various size and fluorescence intensity . The small sized QDs (appr.…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was (i) to detect generation of ROS in the presence of CdS QDs under UV‐C (λ=254 nm) irradiation and (ii) to assess a potential risk of thiol‐capped CdS QDs towards the structure of plasmid (pUC19) and chromosomal (calf thymus) dsDNA utilizing DNA‐based electrochemical biosensors and gel electrophoresis. The 4.0 nm sized CdS QDs preparative has been selected according to our previous study with salmon sperm dsDNA . The spectrophotometric indicator 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD−Cl) and α‐diphenyl‐β‐picrylhydrazyl (DPPH) were used to investigate the ROS formation.…”

Section: Introductionmentioning

confidence: 99%

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Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

et al. 2017

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Different sensitivity of various types of DNA to damage and mutation requires a study of effects of chemical and physical factors on integrity of individual DNA structures. Oxidation stress induced by photoactivated nanoparticles including quantum dots is considered to be the main mechanism of their potential risks. In this work, spectrophotometric indicators of reactive oxygen species (ROS) 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD−Cl) and α‐diphenyl‐β‐picrylhydrazyl (DPPH), electrochemical DNA‐based biosensors with the thiol‐capped CdS quantum dots (CdS QDs) immobilized over the chromosomal (ct DNA) and plasmid (pUC19) DNA biorecognition layers and agarose gel electrophoresis were used (i) to detect ROS generated at the UV‐C irradiation (λ=254 nm) of CdS QDs and (ii) to assess and evaluate their effect towards the chromosomal and plasmid dsDNA structure. Voltammetric and impedimetric measurements revealed a deep degradation of DNA attached to the glassy carbon electrode surface with a stepwise decrease of the portion of survived pUC19 and ct DNA after 60 s to 2400 s irradiation time up to 47 % and to 32 %, resp., comparing to the portion in the absence of CdS QDs reaching 58 % and 40 %, resp. Agarose gel electrophoresis supported the damage to plasmid and chromosomal DNA demonstrating double strand breaks. A decrease in the stability of DNA types towards degradation by ROS in order pUC19>calf thymus>salmon sperm dsDNA has been found.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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