Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system

Sanchez, Zoe; Tani, Akio; Suzuki, Nobuhiro; Kariyama, Reiko; Kumon, Hiromi; Kimbara, Kazuhide

doi:10.1016/j.jbiosc.2012.09.018

Cited by 14 publications

(7 citation statements)

References 25 publications

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“…The microscopy analyses support Xap biofilm architecture changes depending on the culture medium, being less dense structures in XVM2 than LB medium. This modification of biofilm architecture, related with changes in habitats, has already been demonstrated in different bacteria [51][52][53] Clusters have been defined as main units of the biofilm architecture [54]. Herein, small clusters that might contribute to the monolayer structure that progress to a compact multilayer structure, were identified.…”

Section: Discussionmentioning

confidence: 80%

Biofilm Formation in Xanthomonas arboricola pv. pruni: Structure and Development

Sabuquillo

Cubero

2021

Agronomy

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Xanthomonasarboricola pv. pruni (Xap) causes bacterial spot of stone fruit and almond, an important plant disease with a high economic impact. Biofilm formation is one of the mechanisms that microbial communities use to adapt to environmental changes and to survive and colonize plants. Herein, biofilm formation by Xap was analyzed on abiotic and biotic surfaces using different microscopy techniques which allowed characterization of the different biofilm stages compared to the planktonic condition. All Xap strains assayed were able to form real biofilms creating organized structures comprised by viable cells. Xap in biofilms differentiated from free-living bacteria forming complex matrix-encased multicellular structures which become surrounded by a network of extracellular polymeric substances (EPS). Moreover, nutrient content of the environment and bacterial growth have been shown as key factors for biofilm formation and its development. Besides, this is the first work where different cell structures involved in bacterial attachment and aggregation have been identified during Xap biofilm progression. Our findings provide insights regarding different aspects of the biofilm formation of Xap which improve our understanding of the bacterial infection process occurred in Prunus spp and that may help in future disease control approaches.

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Section: Discussionmentioning

confidence: 80%

Biofilm Formation in Xanthomonas arboricola pv. pruni: Structure and Development

Sabuquillo

Cubero

2021

Agronomy

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“…Numerous studies have been carried out to investigate the formation and inhibition of biofilms. In some of these studies, open circulation systems were used to provide biofilm-forming fluids with steady bacteria concentrations (Percival et al 1999;Sanchez et al 2013). However, more chose to place the substrates in closed systems, such as Petri dishes or 96-well plates (Martino et al 2003;Machado et al 2015;Brambilla et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Using micro-patterned surfaces to inhibit settlement and biofilm formation by Bacillus subtilis

et al. 2017

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Biofilm is a biological complex caused by bacteria attachment to the substrates and their subsequent reproduction and secretion. This phenomenon reduces heat transfer efficiency and causes significant losses in treated sewage heat-recovering systems. This paper describes a physical approach to inhibit bacteria settlement and biofilm formation by Bacillus subtilis, which is the dominant species in treated sewage. Here, micro-patterned surfaces with different characteristics (stripe and cube) and dimensions (1-100 μm) were fabricated as surfaces of interest. Model sewage was prepared and a rotating coupon device was used to form the biofilms. Precision balance, scanning electron microscopy, and confocal laser scanning microscopy (CLSM) were employed to investigate the inhibitory effects and the mechanisms of the biofilm-surface interactions. The results have shown that surfaces with small pattern sizes (1 and 2 μm) all reduced biofilm formation significantly. Interestingly, the CLSM images showed that the surfaces do not play a role in "killing" the bacteria. These findings are useful for future development of new process surfaces on which bacteria settlement and biofilm formation can be inhibited or minimized.

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“…The flow system described by Sanchez et al . 17 was modified for evaluation of both bacterial adhesion and biofilm formation. First, the samples were connected to a multichannel pump (IPC8; ISMATEC, Wertheim, Germany) in parallel, and filled with sterile modified artificial urine supplied by the pump from the medium bottle.…”

Section: Methodsmentioning

confidence: 99%

“…To assess biofilm formation, microscopic observations and image acquisitions were performed by CLSM as reported by Sanchez et al . 17 We used a Zeiss LSM 780 (Carl Zeiss Co., Ltd, Oberkochen, Germany) on days 3, 5, 7 and 14 after bacterial inoculation into the samples, which were sufficiently long for the biofilm to mature. 19 3D images and optical z‐sections were generated using LSM ZEN‐software (Carl Zeiss Co., Ltd).…”

Section: Methodsmentioning

confidence: 99%

Intraluminal diamond‐like carbon coating with anti‐adhesion and anti‐biofilm effects for uropathogens: A novel technology applicable to urinary catheters

Watari¹,

Wada²,

Araki³

et al. 2021

Int J of Urology

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Objectives: To examine anti-adhesion and anti-biofilm effects of a diamond-like carbon coating deposited via a novel technique on the inner surface of a thin silicon tube. Methods: Diamond-like carbon coatings were deposited into the lumen of a silicon tube with inner diameters of 2 mm. The surface of the diamond-like carbon was evaluated using physicochemical methods. We used three clinical isolates including green fluorescent protein-expressing Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. We employed a continuous flow system for evaluation of both bacterial adhesion and biofilm formation. Bacterial adhesion assays consisted of counting the number of colony-forming units and visualization of adhered bacterial cells by scanning electron microscope to evaluate the diamond-like carbon-coated/uncoated samples. The biofilm structure was analyzed by confocal laser scanning microscopy on days 3, 5, 7 and 14 for green fluorescent protein-expressing Pseudomonas aeruginosa. Results: The smooth and carbon-rich structure of the intraluminal diamond-like carbon film remained unchanged after the experiments. The numbers of colony-forming units suggested lower adherence of green fluorescent protein-expressing Pseudomonas aeruginosa and Escherichia coli in the diamond-like carbon-coated samples compared with the uncoated samples. The scanning electron microscope images showed adhered green fluorescent protein-expressing Pseudomonas aeruginosa cells without formation of microcolonies on the diamond-like carbon-coated samples. Finally, biofilm formation on the diamond-like carbon-coated samples was lower until at least day 14 compared with the uncoated samples. Conclusions: Intraluminal diamond-like carbon coating on a silicone tube has antiadhesion and anti-biofilm effects. This technology can be applied to urinary catheters made from various materials.

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Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system

Cited by 14 publications

References 25 publications

Biofilm Formation in Xanthomonas arboricola pv. pruni: Structure and Development

Biofilm Formation in Xanthomonas arboricola pv. pruni: Structure and Development

Using micro-patterned surfaces to inhibit settlement and biofilm formation by Bacillus subtilis

Intraluminal diamond‐like carbon coating with anti‐adhesion and anti‐biofilm effects for uropathogens: A novel technology applicable to urinary catheters

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