Assessment of Chicken-Egg Membrane as a Dressing for Wound Healing

Guarderas, Fernando; Leavell, Yaowaree; Sengupta, Trisha; Zhukova, Mariia; Megraw, Timothy L.

doi:10.1097/01.asw.0000480359.58866.e9

Cited by 32 publications

(19 citation statements)

References 5 publications

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“…We have previously demonstrated that this material holds promising anti-inflammatory effects [ 16 ] and activating of epithelization and fibroblast in this study could indicate a broad specificity in treatment. Also, Guarderas et al showed that chicken egg-membrane dressing significantly improved healing of cutaneous wounds in early stages of wound healing in Spraque-Dawley rats [ 1 ]. Cutaneous wound repair and chronical wounds express specific MMP-profiles [ 57 , 58 ], and further studies on effects on MMP-activity in different wound models would be necessary.…”

Section: Discussionmentioning

confidence: 99%

“…It has been used as an alternative natural bandage on burned and cut skin injuries for more than four hundred years in Asian countries. Recently, a study demonstrated an ESM-positive effect in a rat skin wound model, by decreasing wound closure time [ 1 ]. ESM is a highly fibrous material that is readily available as a remnant of the chicken egg processing (breaking) industry, and therefore shows potential as a low-cost ingredient for wound treatment products.…”

Section: Introductionmentioning

confidence: 99%

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Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes

et al. 2018

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Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage to cure wounds, and is available in large quantities from egg industries. We have previously demonstrated that processed eggshell membrane powder (PEP), aiming to be used in a low cost wound healing product, possesses anti-inflammatory properties. In this study, we further investigated effects of PEP on MMP activities in vitro (a dermal fibroblast cell culture system) and in vivo (a mouse skin wound healing model). Three days incubation with PEP in cell culture led to rearrangement of the actin-cytoskeleton and vinculin in focal adhesions and increased syndecan-4 shedding. In addition, we observed increased matrix metalloproteinase type 2 (MMP-2) enzyme activation, without effects on protein levels of MMP-2 or its regulators (membrane type 1 (MT1)-MMP and tissue inhibitor of matrix metalloproteinase type 2 (TIMP-2). Longer incubation (10 days) led to increased protein levels of MMP-2 and its regulators. We also observed an increased alpha-smooth muscle actin (α-SMA) production, suggesting an effect of PEP on myofibroblast differentiation. In vivo, using the mouse skin wound healing model, PEP treatment (3 days) increased MMP activity at the wound edges, along with increased MMP-2 and MMP-9 protein levels, and increased keratinocyte cell proliferation. Altogether, our data suggest PEP stimulates MMP activity, and with a positive effect on early cellular events during wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes

et al. 2018

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“…Providing a scaffold for the fibroblast migration and reducing the lag phase for wound healing [ 119 ]…”

Section: Figurementioning

confidence: 99%

Avian Eggshell Membrane as a Novel Biomaterial: A Review

Shi

Zhou

et al. 2021

Foods

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The eggshell membrane (ESM), mainly composed of collagen-like proteins, is readily available as a waste product of the egg industry. As a novel biomaterial, ESM is attractive for its applications in the nutraceutical, cosmetic, and pharmaceutical fields. This review provides the main information about the structure and chemical composition of the ESM as well as some approaches for its isolation and solubilization. In addition, the review focuses on the role and performance of bioactive ESM-derived products in various applications, while a detailed literature survey is provided. The evaluation of the safety of ESM is also summarized. Finally, new perspectives regarding the potential of ESM as a novel biomaterial in various engineering fields are discussed. This review provides promising future directions for comprehensive application of ESM.

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“…It has been used as an alternative natural bandage on burned and cut skin injuries for >400 years in Asian countries. Recently, a study has demonstrated its positive effect in a rat wound model, by decreasing wound closure time 2. So far, mechanisms of how ESM actually works on wound healing, or the bioactive components involved in this process, are far from understood.…”

Section: Introductionmentioning

confidence: 99%

The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells

Vuong

Rønning

Suso³

et al. 2017

JIR

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Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage on burned and cut skin injuries for >400 years in Asian countries, and is available in large quantities from egg industries. Our aim was to characterize ESM that was separated and processed from egg waste, and to study whether this material possesses anti-inflammatory properties, making it suitable as an ingredient in industrial production of low cost wound healing products. Our results show that the processed ESM particles retain a fibrous structure similar to that observed for the native membrane, and contain collagen, and carbohydrate components such as hyaluronic acid and sulfated glycosaminoglycans, as well as N-glycans, mostly with uncharged structures. Furthermore, both processed ESM powder and the ESM-derived carbohydrate fraction had immunomodulation properties in monocytes and macrophage-like cells. Under inflammatory conditions induced by lipopolysaccharide, the ESM powder and the isolated carbohydrate fraction reduced the activity of the transcription factor nuclear factor-κB. The expression of the immune regulating receptors toll-like receptor 4 and ICAM-1, as well as the cell surface glycoprotein CD44, all important during inflammation response, were down-regulated by these fractions. Interestingly, our experiments show that the two fractions regulated cytokine secretion differently: ESM depressed inflammation by increased secretion of the anti-inflammatory cytokine IL-10 while the carbohydrate fraction reduced secretions of the pro inflammatory cytokines IL-1β and IL-6. Also, the phosphorylation of p65 and p50 subunits of nuclear factor-κB, as well as nuclear localization, differed between processed ESM powder and carbohydrate fraction, suggesting different down-stream regulation during inflammation. In conclusion, processed ESM powder and its soluble carbohydrate components possess anti-inflammatory properties, demonstrating the potential of ESM as a novel biological wound dressing for treatment of chronic inflammatory wounds.

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Assessment of Chicken-Egg Membrane as a Dressing for Wound Healing

Abstract: Chicken-egg membrane dressing significantly improves healing of cutaneous wounds in the early stages of wound healing.

Cited by 32 publications

References 5 publications

Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes

Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes

Avian Eggshell Membrane as a Novel Biomaterial: A Review

The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells

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Assessment of Chicken-Egg Membrane as a Dressing for Wound Healing

Abstract: Chicken-egg membrane dressing significantly improves healing of cutaneous wounds in the early stages of wound healing.

Cited by 32 publications

References 5 publications

Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes

Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes

Avian Eggshell Membrane as a Novel Biomaterial: A Review

The extracellular matrix of eggshell displays anti-inflammatory activities through NF-&kappa;B in LPS-triggered human immune cells

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The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells