Assessment of Chilling Injury and Molecular Marker Analysis in Cucumber Cultivars (Cucumis sativus L.)

Ali, Asjad; Yang, Eun Mi; Bang, Sun Woong; Chung, Sang-Min; Staub, Jack E.

doi:10.7235/hort.2014.13138

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…In case of cucumber, its growth is inhibited at a temperature higher than 35°C and lower than 12°C as well as under stress conditions such as low humidity and high salt concentration (RDA 2013;Ali et al 2014aAli et al , 2014b. Many studies have been carried out to understand the physiological and molecular mechanisms of stress response and to enhance abiotic stress-tolerance in Cucurbitaceae crops.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Lee

Ali

et al. 2017

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Dehydrins (DHNs) are hydrophilic proteins with conserved lysine-rich K-segment, which belong to Group II of the late embryogenesis abundant (LEA) protein family. DHNs are considered as molecular chaperons playing important roles in abiotic stress tolerance. In this study, DHN genes were identified through genome-wide searches in five Cucurbitaceae species, including cucumber, wild cucumber, melon, watermelon, and bitter gourd. Three to five DHN genes were found in each of the five species, which were further divided into several protein architecture types based on the presence and order of the major conserved motifs such as K-, Y-, and S-segments. In silico expression profiling using RNA-Seq data revealed high expression of SK3-type DHN gene and low expression of other type DHN genes in cucumber and melon. In silico promoter analysis identified a number of cis-acting element-like sequences related to abiotic stress-response such as DRE and ABRE in 2-kb putative promoter sequences. DHN genes identified in this study will be valuable for understanding the stress response mechanism as well as assisting molecular breeding in Cucurbitaceae crops.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Lee

Ali

et al. 2017

Plant Breed. Biotech.

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“…Therefore, the subsequent model could be configured with 4 centroids, each corresponding to an unstressed state, slight chilling injury, moderate chilling injury, and severe chilling injury, respectively. This scheme also referred to previous literature ( Ali et al., 2014 ).…”

Section: Resultsmentioning

confidence: 89%

A classification method of stress in plants using unsupervised learning algorithm and chlorophyll fluorescence technology

Lu,

Gao,

et al. 2023

Front. Plant Sci.

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IntroductionChilling injury is one of the most common meteorological disasters affecting cucumber production. For implementing remedial measures as soon as possible to minimize production loss, a timely and precise assessment of chilling injury is crucial.MethodsTo evaluate the possibility of detecting cucumber chilling injury using chlorophyll fluorescence (ChlF) technology, we investigated the continuous changes in ChlF parameters under various low-temperature conditions and created the criteria for evaluating chilling injury. The ChlF induction curves were first collected before low-temperature as unstressed samples and daily 1 to 5 days after low-temperature as chilling injury samples. Principal component analysis was employed to investigate the public information on ChlF parameters and evaluate the differences between samples with different degrees of chilling injury. The parameters (Fv/Fm, Y(NO), qP, and Fo) accounted for a large proportion in the principal components and could characterize chilling injury. Uniform manifold approximation and projection method was employed to extract new features (Feature 1, Feature 2, Feature 3, and Feature 4) from ChlF parameters for subsequent classification model. Taking four features as input, a classification model based on the Fuzzy C-means clustering algorithm was constructed in order to identify the chilling injury classes of cucumber seedlings. The cucumber seedlings with different chilling injury classes were analyzed for ChlF images, rapid light curves, and malondialdehyde content.Results and discussionThe results demonstrated that the variations in these indicators among the different chilling injury classes supported the validity of the classification model. Our findings provide a better understanding of the relationship between ChlF parameters and the impact of low-temperature treatment on cucumber seedlings. This finding offers an additional perspective that can be used to evaluate the responses and damage that plants experience under stress.

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“…There have been many reports about cold tolerance identification in cucumbers, but the number of germplasms is limited in most studies. For example, Ali et al compared cold tolerance of 12 commercial cucumber varieties in Korea [ 36 ]. In general, cold-tolerant cucumbers are rare in germplasm and the proportion of cold-tolerant cucumber is lower than 10% [ 35 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber

Salah

Zhang

Xia

et al. 2022

Plants

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Cucumbers (Cucumis sativus L.) originated from the South Asian subcontinent, and most of them are fragile to cold stress. In this study, we evaluated the cold tolerance of 115 cucumber accessions and screened out 10 accessions showing high resistance to cold stress. We measured and compared plant hormone contents between cold-tolerant cucumber CT90R and cold-sensitive cucumber CT57S in cold treatment. Most of the detected plant hormones showed significantly higher content in CT90R. To elucidate the role of plant hormones, we compared the leaf- and root-transcriptomes of CT90R with those of CT57S in cold stress treatment. In leaves, there were 1209 differentially expressed genes (DEGs) between CT90R and CT57S, while there were 703 in roots. These DEGs were not evenly distributed across the chromosomes and there were significant enrichments at particular positions, including qLTT6.2, a known QTL controlling cucumber cold tolerance. The GO and KEGG enrichment analysis showed that there was a significant difference in the pathway of plant hormone transductions between CT90R and CT57S in leaves. In short, genes involved in plant hormone transductions showed lower transcription levels in CT90R. In roots, the most significantly different pathway was phenylpropanoid biosynthesis. CT90R seemed to actively accumulate more monolignols by upregulating cinnamyl-alcohol dehydrogenase (CAD) genes. These results above suggest a new perspective on the regulation mechanism of cold tolerance in cucumbers.

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Assessment of Chilling Injury and Molecular Marker Analysis in Cucumber Cultivars (Cucumis sativus L.)

Cited by 6 publications

References 27 publications

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

A classification method of stress in plants using unsupervised learning algorithm and chlorophyll fluorescence technology

Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber

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