Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers

Bhatia, Reeta; Singh, Kanwar Pal; Jhang, Tripta; Sharma, Tilak Raj

doi:10.1016/j.scienta.2008.07.024

Cited by 115 publications

(56 citation statements)

References 20 publications

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“…An example of PCR amplification products obtained with the primer ASSR-15 is given in Figure 1f. This findings agree with those reported by others (Lakshmanan et al 2007;Ahmad et al 2008;Bhatia et al 2009) who have pointed out the efficacy of 3'-anchored primers for giving a higher number of clearly definable bands and being better than other markers such as RAPDs in the assessment of clonal fidelity of micropropagated plants (Martins et al 2004). …”

Section: Issr Analysissupporting

confidence: 92%

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Arzate-Fernández¹,

Piña-Escutia²,

Vázquez-García³

2010

Electro Journal of Biotech

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Section: Issr Analysissupporting

confidence: 92%

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Arzate-Fernández¹,

Piña-Escutia²,

Vázquez-García³

2010

Electro Journal of Biotech

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“…PLB proliferation of Grammatophyllum speciosum, in ½MS liquid medium supplemented with 15 mg/L chitosan led to a 7-fold increase in PLB growth (Sopalun et al, 2010). Similarly, in the case of Dendrobium phalaenopsis the addition (Bhatia et al, 2009), the plantlets generated from in vitro cultured banana regenerated from rhizomes (Lakshmanan et al, 2007), almond plantlets regenerated from axillary branches (Martins et al, 2004), a monopodial orchid hybrid with several shoots initiated from seedlings (Kishor and Devi, 2009) and Cymbopogon flexuosus initiated from somatic embryogenesis (rhizomatous explants) (Bhattacharya et al, 2008). The proliferation system reported here is efficient and capable of producing large numbers of genetically uniform Phalaenopsis gigantea PLBs within a relatively short period of time.…”

Section: Effects Of Different Chitosan Concentrations and Media Typesmentioning

confidence: 91%

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Samarfard¹,

Kadir²,

Kadzimin³

et al. 2013

Not Bot Hort Agrobot Cluj

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Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.

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“…A espécie é uma das mais importantes culturas no comércio mundial de fl ores de corte e de vaso, estando em quinto lugar no ranking (BHATIAA et al, 2009).…”

Section: Introductionunclassified

Conservantes florais comerciais nas soluções de manutenção de hastes florais de gérbera de corte

et al. 2014

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As flores de corte são produtos altamente perecíveis e sua vida pós-colheita deve ser prolongada ao máximo para garantir a fidelidade dos consumidores. Uma possível medida para ampliar o período pós-colheita é a adição de fontes exógenas como biocidas, açúcares e substâncias acidificantes, compostos presentes nos conservantes florais comerciais. Nesse sentido, objetivou-se avaliar a eficácia do uso de conservantes florais na longevidade pós-colheita e na redução do número de bactérias em hastes florais de gérbera de corte. Seis hastes florais por unidade experimental de gérberas 'Essandre', colhidas em produtor comercial, foram condicionadas em frascos de vidro com 1L das seguintes soluções: T1 - água destilada (tratamento testemunha); T2-1,0% (v/v) Flower(r); T3-1,5% (v/v) Flower(r); T4-1,0% (v/v) Flower Plus(r); T5-1,5% (v/v) Flower Plus(r); e T6-1 tablete de Florissant 500(c). Ao final de 7 dias, observou-se o conteúdo de carboidratos totais e, ao final do experimento: a longevidade; a massa fresca relativa; e a absorção de solução. Nas soluções de vaso, durante o período experimental, avaliou-se o pH. A contagem bacteriana, nas hastes florais, foi realizada com os tratamentos: T1 - água destilada (tratamento testemunha); T2-0,75% (v/v) Flower(r); T3-1,5% (v/v) Flower(r). Os conservantes florais testados não aumentam a longevidade pós-colheita de hastes florais de gérbera de corte. O uso de conservante floral reduz o número de bactérias em hastes florais de gérbera de corte.

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Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers

Cited by 115 publications

References 20 publications

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Conservantes florais comerciais nas soluções de manutenção de hastes florais de gérbera de corte

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