Assessment of Deoxyribonuclease Activity Using DNA Molecules Immobilized Between Microelectrodes

Himuro, Takahiro; Saito, Yoji

doi:10.1007/s11664-020-08596-8

Cited by 2 publications

(2 citation statements)

References 34 publications

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“… 28 Additionally, we developed a DNase-detecting device using multiple λDNA nanowires and performed several experiments using rod-shaped parallel electrodes. 29 The device comprised a PDMS reservoir with a volume of 400 μL and two aluminum electrodes. This method enabled a simple and highly reproducible detection of DNase without DNA pretreatment or electrode surface modification.…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study, we investigated the electrical properties of lambda phage DNA (λDNA) molecules, which were manipulated by electrostatic orientation, based on their current–voltage characteristics and complex impedance plots . Additionally, we developed a DNase-detecting device using multiple λDNA nanowires and performed several experiments using rod-shaped parallel electrodes . The device comprised a PDMS reservoir with a volume of 400 μL and two aluminum electrodes.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of a Deoxyribonuclease Sensor Based on the Electrical Characteristics of DNA Molecules

2022

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In this study, we developed a sensing device that can detect deoxyribonuclease (DNase) based on the electrical properties of deoxyribonucleic acid (DNA). We estimated the equivalent circuit between the electrodes with immobilized DNA and investigated whether the characteristics of the electrodes change before and after the DNase reaction. This method detects DNase by simply evaluating the electrical properties of DNA without using a fluorescent reagent. Therefore, inexpensive and highly accurate measurements can be performed with simple operations. However, detection sensitivity must be increased for practical feasibility. Hence, we investigated whether DNA immobilization is restricted by changing the shape of the electrode to a triangle with sharp edges, which may improve the sensitivity of DNase. Additionally, we attempted to detect DNase from an extremely small amount of sample solution using a microchannel. The device was able to quantitatively analyze DNase I activity with a detection limit of 5.5 × 10 –5 unit/μL. The results demonstrate the effectiveness of the proposed sensing device for various medical applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of a Deoxyribonuclease Sensor Based on the Electrical Characteristics of DNA Molecules

2022

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A Facile Label‐Free Colorimetric Assay for DNase I Activity Based on the Peroxidase‐Like Activity of Functional Nucleic Acids

Song,

Yan,

Sun

et al. 2024

ChemBioChem

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DNase I plays a crucial role in fundamental biological processes, including gene expression, DNA repair, and apoptosis. Therefore, it is important to develop a simple and efficient method to detect DNase I activity. In this work, a colorimetric assay based on functional nucleic acid for sensitive DNase I detection was reported. The functional nucleic acids with G‐rich bases exhibit peroxidase‐like activity under acetic acid conditions, capable of catalyzing the oxidation of chromogenic substrate 3,3',5,5'‐tetramethylbenzidine (TMB). The specific nucleic acid is designed as the substrate for DNase I, which can hydrolyze the strand and reduce the catalytic activity of the reaction system. Based on this, a sensitive, simple, efficient, and cost‐effective colorimetric detection platform was constructed for the quantitative analysis of DNase I. The detection limit (3σ/s) for DNase I was 0.046 U/mL with a linear range of 0.1–1 U/mL. Moreover, the practicality of this colorimetric detection platform was demonstrated by detecting DNase I in human serum.

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Assessment of Deoxyribonuclease Activity Using DNA Molecules Immobilized Between Microelectrodes

Cited by 2 publications

References 34 publications

Fabrication of a Deoxyribonuclease Sensor Based on the Electrical Characteristics of DNA Molecules

Fabrication of a Deoxyribonuclease Sensor Based on the Electrical Characteristics of DNA Molecules

A Facile Label‐Free Colorimetric Assay for DNase I Activity Based on the Peroxidase‐Like Activity of Functional Nucleic Acids

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