Assessment of different storage conditions for Staphylococcus hyicus survival

Abstract

Introduction: Collecting swabs from skin lesions for bacteriological examination is frequently performed to the diagnosis of exudative epidermitis. This method is fast and non-invasive, but it depends directly on the viability of bacteria in clinical samples, which can be influenced by storage and shipment temperatures and the time of transportation. The aim of this study was to assess the capacity of four commercial transport media and swabs with no transport medium to preserve Staphylococcus hyicus (S. hyicus) for up to 10 days at room temperature and under refrigeration. Methodology: Samples were stored in swabs with no transport medium and four transport media (Amies, Amies with charcoal, Cary Blair and Stuart) for 10 days at room temperature and under refrigeration. Swabs were plated in Tween 80 Agar and colonies counted. Results: Samples kept in transport media showed better performance (P < 0.05) under refrigeration. Storage under refrigeration in Amies medium showed better results than all other transport media and swabs (P < 0.05). Amies medium and swabs with no transport medium showed comparable results in room temperature (P > 0.05). In additional, refrigerated Amies medium and swabs with no transport medium at room temperature showed high performance for up to nine and three storage days, respectively. Conclusions: The recovery of S. hyicus in samples stored in Amies medium under refrigeration was higher when compared to other transport media. In addition, swabs with no transport medium could also be indicated when samples are stored at room temperature within three days.

Diagnosis of Renibacterium salmoninarum infection in harvested Atlantic salmon (Salmo salar L.) on the east coast of Canada: Clinical findings, sample collection methods and laboratory diagnostic tests

“…On the contrary to our findings, the addition of a transport medium, such as Amies or Stuart medium, has been shown to better preserve bacterial species by inactivating toxic substances or by keeping the swab fibres moist during transport to the laboratory (Barber et al, 1998; Barry et al, 1972; Drake et al, 2005). Some other studies reported the performance of Amies with‐ or without‐charcoal and Stuart media are likely to be confounded by storage temperature and time (Cumming & Ross, 1979; Takeuti et al, 2018). To the authors' knowledge, the present study is the first to demonstrate the differences in specimen type in the recovery of R. salmoninarum using bacterial culture.…”

Ексудативний Епідерміт Свиней (Етіологія, Прояви Інфекції, Заходи Боротьби)

“…Забір мазків з уражень шкіри для бактеріологічного дослідження проводять з застосуванням транспортного середовища Еймса в охолодженому стані (Takeuti et al, 2018).…”