Assessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity

Santos, Fábio V.; Cólus, I.M.S.; Silva, M. A.; Vilegas, Wagner; Varanda, Eliana Aparecida

doi:10.1016/j.fct.2006.03.012

Cited by 68 publications

(43 citation statements)

References 26 publications

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“…A sample was considered mutagenic when a dose-response relationship was detected and a twofold increase in the number of mutants (MI ‡ 2) was observed for at least one concentration. 24 The standard mutagens used as positive controls in experiments in the absence of the S9 mix were 4-nitro-o-phenylenediamine (10 lg/plate) for TA98 and TA97a, sodium azide (2.5 lg/plate) for TA100, and mitomycin (0.5 lg/plate) for TA102. 2-Anthramine (1.25 lg/plate) was used with TA98, TA97a, and TA100, whereas 2-aminofluorene (10 lg/plate) was used with TA102 in the experiments with metabolic activation.…”

Section: Mutagenicity Assaysmentioning

confidence: 99%

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Costa

Rinaldo²,

Varanda³

et al. 2014

Journal of Medicinal Food

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It is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography-diode array detection analysis method for the simultaneous detection of myricetin-3-O-b-Dgalactopyranoside, myricetin-3-O-a-L-arabinopyranoside, and myricetin-3-O-a-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83-107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.

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Section: Mutagenicity Assaysmentioning

confidence: 99%

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Costa

Rinaldo²,

Varanda³

et al. 2014

Journal of Medicinal Food

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“…(Melastomataceae), Davilla elliptica St. Hill. (Dilleniaceae), and Byrsonima crassa Niedenzu (IK) (Malpighiaceae) are native trees of the savannah-like Brazilian cerrado (Sannomiya et al, 2004;Andreo et al, 2006;Santos et al, 2006). All of them are used in popular medicine for the treatment of gastrointestinal disturbances and infl ammatory processes.…”

Section: Introductionmentioning

confidence: 99%

Isolated Flavonoids against Mammary Tumour Cells LM2

Carli

Matos

Lopes

et al. 2009

Zeitschrift Für Naturforschung C

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The purpose of the present study was to investigate antitumour and anti-infl ammatory activities of fl avonoids isolated from Byrsonima crassa, Davilla elliptica and Mouriri pusa. The antitumour activity was measured by the MTT assay in murine mammary tumour cells (LM2) and the IC 50 values of the fl avonoids tested ranged from (31.5 ± 2.97) to (203.1 ± 5.9) μg/ml. The fl avonoids 1 (myricetin-3-O-α-L-rhamnopyranoside) and 3 (quercetin-3-Ogalactopyranoside) from D. elliptica were the most active ones against the tumour cells. The same samples were tested to determine the inhibition of the release of nitric oxide (NO) and of the tumour necrosis factor-alpha (TNF-α) in murine macrophages by the Griess and ELISA sandwich assay, respectively. Almost all the samples showed inhibitory activity to the release of NO but not of TNF-α. Of all substances tested, fl avonoids 2 (quercetin) and 6 (myricetin) may show promising activity in the treatment of murine breast cancer by immunomodulatory and antiproliferative activities.

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“…In this regard, several antioxidant rich plants have been reported for their protective property against DNA damage in human lymphocytes [Rani et al, 2005;Santos et al, 2006]. Duthie et al [1997] reported that the dietary flavanoids, quercetin, and myrecetin, reduced DNA damage in human lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL‐60 cell line

Ramkumar¹,

Sankar²,

Manjula³

et al. 2009

Environ and Mol Mutagen

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In this study we have evaluated the genoprotective effect of the ethanol extract of Gymnema montanum (GLEt) leaves in human peripheral blood lymphocytes and HL-60 cell line in vitro using the comet assay. DNA damage was induced by treating the cells with H(2)O(2) and methyl methane sulphonate (MMS). GLEt treatment effectively protected the lymphocytes and HL-60 cell line from H(2)O(2)-induced oxidative DNA damage in a dose-dependent manner whereas it was not effective against alkylative DNA damage caused by MMS. The global percent repair efficiency also showed that both pre- and post- GLEt treatment provided effective protection against H(2)O(2) induced DNA damage but not as effective against MMS. At 200 microg ml(-1) level, its repair capacity against H(2)O(2) induced DNA damage was comparable to that of vitamin-C (100 microM). Furthermore, exposure to GLEt reduced the formation of apoptotic cells caused by H(2)O(2), which was demonstrated by the decreased sub-G1-DNA content in cell cycle analysis and apoptotic frequencies of lymphocytes in an annexin-V binding assay. In addition, GLEt was found to have effective peroxide scavenging ability in dose-dependent manner. The protective efficiency of the extract was found to be directly proportional to its total phenolic content. The present study indicates that G. montanum leaves are a significant source of phytochemicals with antigenotoxic and antioxidant activity, and thus has potential therapeutic use.

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Assessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity

Cited by 68 publications

References 26 publications

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Isolated Flavonoids against Mammary Tumour Cells LM2

Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL‐60 cell line

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