This study evaluated the implementation of infection prevention standards in the emergency departments of selected hospitals. Data was collected from physicians and nurses using a questionnaire assessing multiple dimensions of infection control measures. Responses from 322 participants were statistically analyzed for validity and stability using Pearson’s coefficient and Cronbach’s alpha. Findings revealed that, while infection control standards were largely implemented across all dimensions, gaps were identified. Specifically, a lack of sterilization indicators, failure to isolate respiratory patients, and inadequate patient and family education were noted. Infection control practices require strengthening, especially isolation procedures. Study outcomes provide healthcare professionals and policymakers with evidence to further improve infection prevention controls and reduce healthcare-associated infection risks, especially during the spread of epidemics such as the COVID-19 epidemic. Enhancing measures in line with the study's recommendations can help create safer care environments in hospitals.