Introduction: Metallo-β-lactamase production among Pseudomonas aeruginosa is a major health problem worldwide. Pseudomonas aeruginosa acquire several mechanisms of resistance towards carbapenems through the production of metallo-β-lactamases, especially VIM and IMP. The problem of multi-drug-resistant Pseudomonas aeruginosa is increasing all over the world, reaching dangerous levels. The aim of this study was to detect the metallo-β-lactamases bla VIM and bla IMP genes in Pseudomonas aeruginosa strains in Suez Canal University Hospital in Ismailia, Egypt. Material and methods: A cross-sectional descriptive study was conducted on 65 Pseudomonas aeruginosa strains. Genotypic detection of bla VIM and bla IMP was reached by using polymerase chain reaction. Results: Out of 65 Pseudomonas aeruginosa strains , bla VIM gene was present in four females and one male, with an age of 42.9 ±18.1; two cases were isolated from the Oncology Department, and one case each was present in the Burn Unit, Surgery Ward, and Intensive Care Unit. The bla VIM gene was expressed in four stains, while the bla IMP gene was not expressed in any strain. Conclusions: The carbapenem resistance in our patients can be referred to as metallo-β-lactamases bla VIM type. The problem of metallo-β-lactamases and carbapenem resistance requires ongoing surveillance, strong preventive measures, and implementation of infection control policies and procedures. Also, routine diagnostic laboratory methods should be performed, and synthesis of antimicrobial products with new effecting mechanism should be implemented in hospitals.

Introduction: Hepatitis C virus is a blood-borne infection and one of the major global problems. In Egypt, the prevalence is reported to be the highest. Infection through unsafe medical procedures is considered now the primary cause of infection. The study aims to assess the knowledge, attitude and practice of doctors regarding infection control guidelines in Ismailia governorate. Material and methods: A cross sectional study was conducted on a sample of physicians (355) in Ismailia governorate using a questionnaire to collect data on their knowledge and attitude concerning the control measures related to their years of experiences. Results: Most of the physicians (89.6%) regardless of their years of experience have had contact with blood products. Those who had a history of needle stick injury accounted for 56.3%, with a statistically significant difference as to the highest number being middle career physicians. Concerning knowledge about infection control policies in their hospitals, it was relatively low among all groups. Conclusions: Despite all efforts exerted by the infection control professionals, infections still remain a major unpleasant side effect of health care, often causing harm to patients.. Although Egypt has made great progress in implementing the infection control procedures and policies, there is still a huge problem as the practices of physicians do not really reflect their knowledge and they still do not follow the infection control policies. Education, monitoring, improved availability of resources and disciplinary measures are necessary to improve infection control in health facilities that should be applied to all physicians.

Background: Healthcare workers are at increased risk of contracting and spreading hepatitis C virus (HCV), and HBV to others. Objective: The aim of study is to evaluate nursing staff and paramedics knowledge, concerning HCV, HBV and its relationship to institutional infection control facilities. Methodology: A self- administered questionnaire was distributed at fever hospital, infectious diseases hospital, Suez Canal university hospital, blood center, primary health care centers and hospitals affiliated to ministry of health, with a convenient sample of 479 nursing staff and paramedics in Suez Canal region. A knowledge score was developed about hepatitis B, C source of infections and different domains of infection control facilities and procedures, with a maximum of 17. Results: The mean age of the participating nursing staff and paramedics was 29.55 + 8. 31. Knowledge score about mode of transmission of hepatitis, reveals that the highest level was among the student nurse which was 17.07. Concerning exposure including blood contact, blood spills and needle stick, the nurse was 61 %, while mainly head nurse to be 83% stated receiving training about infection control and care of sharp. Conclusion: We can prevent the transmission of infection at the occupational level through standard precautions.

Background: Speech and language delay in children present with delayed milestones and therefore difficulty with expression initially, as well as social skills, reading and writing. The cause of delayed speech is difficult to determine in many cases. Objective: to study the role of allergy that would influence the speech delay in children.