Assessment of environmental stress in Parablennius sanguinolentus (Pallas, 1814) of the Sicilian Ionian coast

Tigano, Concetta; Tomasello, Barbara; Pulvirenti, Valentina; Ferrito, Venera; Copat, Chiara; Carpinteri, Guido; Mollica, Emanuele; Sciacca, Salvatore; Renis, Marcella

doi:10.1016/j.ecoenv.2008.09.028

Cited by 27 publications

(16 citation statements)

References 63 publications

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“…1 The comet scale of hepatic cells induced by 8-HOQ in Cryprinus carpio (9400), a four-class classification based on tail moment adopted from Anderson et al (1994) and Nan et al (2013a, b (Nan et al 2013a, b). This was similar to the research results on aminotransferase responses to other poisonous substances (Tepe et al 2008;Tigano et al 2009;Yin et al 2011;Saravanan et al 2012), such as changes in cytomembrane permeability of hepatic cells following seepage of GPT and GOT from hepatic tissue into blood plasma. It is important to note that hepatic GOT and GPT activities may be potential biomarkers of 8-HOQ toxicity, consistent with pesticide persistence in fish observed here and requires further study.…”

Section: Resultssupporting

confidence: 75%

Toxicity of 8-Hydroxyquinoline in Cryprinus carpio Using the Acute Toxicity Test, Hepatase Activity Analysis and the Comet Assay

Yan

Chen

Dou

et al. 2015

Bull Environ Contam Toxicol

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To evaluate the environmental toxicity of 8-hydroxyquinoline (8-HOQ), an important industrial raw material found in China's major ornamental fish, Cryprinus carpio, using the acute toxicity test, hepatase activity analysis and the comet assay. The results indicated that 8-HOQ had significant acute toxicity in adult C. carpio with a 96 h-LC50 of 1.15 and 0.22 mg L(-1) hepatic quinoline residues as assessed by HPLC. 8-HOQ also induced genotoxicity in the form of strand breaks in the DNA of hepatic cells as shown by the comet assay. With regard to physiological toxicity, 8-HOQ induced a decrease in the activities of hepatic GOT and GPT with increased exposure concentration and time. These data suggest that 8-HOQ may be toxic to the health of aquatic organisms when accidentally released into aquatic ecosystems. The data also suggest that the comet assay may be used in biomonitoring to determine 8-HOQ genotoxicity and hepatic GPT and GOT activities may be potential biomarkers of physiological toxicity.

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Section: Resultssupporting

confidence: 75%

Toxicity of 8-Hydroxyquinoline in Cryprinus carpio Using the Acute Toxicity Test, Hepatase Activity Analysis and the Comet Assay

Yan

Chen

Dou

et al. 2015

Bull Environ Contam Toxicol

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“…However, our experience with WBCs, utilizing typical and atypical comet assay versions, confirms that whole blood cells, if opportunely treated, maybe preferred to lymphocytes. 9,14,21 Conversely to Andreoli et al, 26 in fact, we observed that the treatment with 200 lM H 2 O 2 at 4°C for 20 min on agarose-embedded whole blood cells (atypical comet assay) was able to induce DNA damage in the runners' white cells, confirming that interference between treatments in situ and RBCs could be bypassed using adequate concentrations of substances.…”

contrasting

confidence: 70%

“…This method also permits evaluating different kinds of DNA damage induced by endogenous and/or exogenous factors, as well as the effects of some antioxidant supplementations and therapies. 11,[13][14][15] Natural antioxidant molecules have been extensively considered in preventing and/or reducing oxidative stress and DNA damage. 7,8,16 Among natural compounds, 3,5,4 0 -trihydroxystilbene (RESV), found in various plants, has attracted particular attention in the last decade for its innumerable properties.…”

Section: Introductionmentioning

confidence: 99%

Double-Face Activity of Resveratrol in Voluntary Runners: Assessment of DNA Damage by Comet Assay

Tomasello

Grasso

Malfa

et al. 2012

Journal of Medicinal Food

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Voluntary runners are subjected to a massive increase in reactive oxygen/nitrogen species production, which can promote different oxidative stress-related diseases such as premature aging, neurodegenerative disorders, and cancer. The aims of this work were to evaluate the following in peripheral blood cells of voluntary runners: (i) DNA status; (ii) susceptibility to the in vitro insult induced by hydrogen peroxide (H(2)O(2)) as a breaking agent; (iii) capabilities of 3,5,4'-trihydroxystilbene (RESV) in counteracting DNA damage. Twenty-five male voluntary runners were compared with 20 sedentary men, as age-matched controls, and DNA status was evaluated with different versions of comet assay: alkaline, neutral, and Fpg enzyme-modified version to measure 8-OH-deoxyguanosine (8-oxo-dG) levels. The H(2)O(2) and/or RESV treatments were performed directly on agarose-embedded cells (atypical comet assay). The results evidenced DNA damage and levels of 8-oxo-dG higher in runners than in sedentary control subjects. The runners' DNA was more prone to the in vitro-induced oxidative insult (200 μM H(2)O(2)) than that of the control group. Resveratrol (100 μM), depending on the individual basal DNA status, was able to switch from antioxidant to pro-oxidant. Our results, on the one hand, validated the proposed in vitro experimental protocol in order to measure individual DNA status. On the other hand, our data point out the importance of monitoring the athletes' redox status before subjecting them to dietary supplementation treatment.

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“…In five of the eight studies HSP70 activity was upregulated, with four assessing the response of fish to contaminated sediment and one examining the response after fish had been exposed to Cd [104, 129–132]. This suggest that this biomarker is suitable for Gladstone Harbour, but further field studies are required to validate its use given the reported seasonal variation in HSP70 activity [133], in combination with the strong influence of climate on HSP70 [5]. …”

Section: Resultsmentioning

confidence: 99%

A protocol for identifying suitable biomarkers to assess fish health: A systematic review

2017

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BackgroundBiomarkers have been used extensively to provide the connection between external levels of contaminant exposure, internal levels of tissue contamination, and early adverse effects in organisms.ObjectivesTo present a three-step protocol for identifying suitable biomarkers to assess fish health in coastal and marine ecosystems, using Gladstone Harbour (Australia) as a case study.MethodsPrior to applying our protocol, clear working definitions for biomarkers were developed to ensure consistency with the global literature on fish health assessment. First, contaminants of concern were identified based on the presence of point and diffuse sources of pollution and available monitoring data for the ecosystem of interest. Second, suitable fish species were identified using fisheries dependent and independent data, and prioritised based on potential pathways of exposure to the contaminants of concern. Finally, a systematic and critical literature review was conducted on the use of biomarkers to assess the health of fish exposed to the contaminants of concern.Results/DiscussionWe present clear working definitions for bioaccumulation markers, biomarkers of exposure, biomarkers of effect and biomarkers of susceptibility. Based on emission and concentration information, seven metals were identified as contaminants of concern for Gladstone Harbour. Twenty out of 232 fish species were abundant enough to be potentially suitable for biomarker studies; five of these were prioritised based on potential pathways of exposure and susceptibility to metals. The literature search on biomarkers yielded 5,035 articles, of which 151met the inclusion criteria. Based on our review, the most suitable biomarkers include bioaccumulation markers, biomarkers of exposure (CYP1A, EROD, SOD, LPOX, HSP, MT, DNA strand breaks, micronuclei, apoptosis), and biomarkers of effect (histopathology, TAG:ST).ConclusionOur protocol outlines a clear pathway to identify suitable biomarkers to assess fish health in coastal and marine ecosystems, which can be applied to biomarker studies in aquatic ecosystems around the world.

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Assessment of environmental stress in Parablennius sanguinolentus (Pallas, 1814) of the Sicilian Ionian coast

Cited by 27 publications

References 63 publications

Toxicity of 8-Hydroxyquinoline in Cryprinus carpio Using the Acute Toxicity Test, Hepatase Activity Analysis and the Comet Assay

Toxicity of 8-Hydroxyquinoline in Cryprinus carpio Using the Acute Toxicity Test, Hepatase Activity Analysis and the Comet Assay

Double-Face Activity of Resveratrol in Voluntary Runners: Assessment of DNA Damage by Comet Assay

A protocol for identifying suitable biomarkers to assess fish health: A systematic review

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