Assessment of estrogenic activity of flavonoids from Mediterranean plants using an in vitro short-term test

Garritano, Sonia; Pinto, Barbara; Giachi, Isa; Pistelli, Luisa; Reali, Daniela

doi:10.1016/j.phymed.2004.01.004

Cited by 26 publications

(26 citation statements)

References 14 publications

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“…Vegetables, fruits and nuts are the most important source of fibers and chemical compounds, like flavonoids, phytosterols, vitamins, terpenes and phenols, which give protection against oxidative processes, hence reducing the incidence of cardiovascular diseases (CVD) [6,7,8,9]. Furthermore, the consumption of olive oil, as the predominant fat intake, provides high oleic acid content and polyphenols, which have atherogenic, antioxidant and anti-inflammatory effects, reducing the cholesterol/high density lipoprotein (HDL) ratio and the concentration of the oxidized low density lipoprotein (LDL) [10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Mediterranean Diet and Health: Food Effects on Gut Microbiota and Disease Control

Chierico

Vernocchi

Dallapiccola

et al. 2014

IJMS

170

112

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The Mediterranean diet (MD) is considered one of the healthiest dietary models. Many of the characteristic components of the MD have functional features with positive effects on health and wellness. The MD adherence, calculated through various computational scores, can lead to a reduction of the incidence of major diseases (e.g., cancers, metabolic and cardiovascular syndromes, neurodegenerative diseases, type 2 diabetes and allergy). Furthermore, eating habits are the main significant determinants of the microbial multiplicity of the gut, and dietary components influence both microbial populations and their metabolic activities from the early stages of life. For this purpose, we present a study proposal relying on the generation of individual gut microbiota maps from MD-aware children/adolescents. The maps, based on meta-omics approaches, may be considered as new tools, acting as a systems biology-based proof of evidence to evaluate MD effects on gut microbiota homeostasis. Data integration of food metabotypes and gut microbiota “enterotypes” may allow one to interpret MD adherence and its effects on health in a new way, employable for the design of targeted diets and nutraceutical interventions in childcare and clinical management of food-related diseases, whose onset has been significantly shifted early in life.

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Section: Introductionmentioning

confidence: 99%

Mediterranean Diet and Health: Food Effects on Gut Microbiota and Disease Control

Chierico

Vernocchi

Dallapiccola

et al. 2014

IJMS

170

112

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“…2A and 2B). In particular, luteolin which did not display any significant estrogenic activity in a previous work (β-gal activity = 4.20% ± 0.75, Garritano et al, 2005), inhibited (77%) estradiol-induced enzymatic activity at the higher concentration tested, and this result is in agreement both with the literature data reporting an inhibitory action of this compound on in vitro proliferation of MCF-7 cells and in vivo uterine hypertrophy in rats (Markaverich et al, 1988) and with the observation that compounds that possess weaker estrogenic activities may exert more appreciable inhibitory action especially at high concentrations (Hwang et al, 2006).…”

Section: Resultsmentioning

confidence: 65%

“…Since luteolin and luteolin 7-glucoside confer on the yeast culture a yellow colour which persists during the assay, the experimental protocol was modified to test antagonistic activity and the chromogenic substrate o-nitrophenyl β-D-galactopyranoside (ONPG) was substituted with chlorophenol red-β-D-galactopyranoside (CPRG) (Clontech, 1999;Garritano et al, 2005).…”

Section: Phytochemicals the Pure Compounds Isoprunetinmentioning

confidence: 99%

Estradiol‐antagonistic activity of phenolic compounds from leguminous plants

Pinto¹,

Bertoli²,

Noccioli³

et al. 2007

Phytotherapy Research

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Natural flavonoids are currently receiving much attention because of their estrogenic and antiestrogenic properties. Six isoflavones (isoprunetin, isoprunetin 7-O-beta-D-glucopyranoside, isoprunetin 4',7-di-O-beta-D-glucopyranoside, genistein, genistein 7-O-beta-D-glucopyranoside, daidzein), four flavones (luteolin, luteolin 7-O-beta-D-glucopyranoside, luteolin 4'-O-beta-D-glucopyranoside, licoflavone C), isolated from Genista morisii and G. ephedroides (two Leguminosae plants of the Mediterranean area) together with two structurally related pterocarpans, bitucarpin A and erybraedyn C, isolated from Bituminaria bituminosa (Leguminosae), were tested for the antagonist activity by a yeast based estrogen receptor assay (Saccharomyces cerevisiae RMY326 ER-ERE). Most compounds inhibited the estradiol-induced transcriptional activity in a concentration dependent manner. In particular, for the flavone luteolin 77% inhibition of the induced beta-galactosidase activity was observed. Interestingly, licoflavone C exhibited a dose-dependent antagonistic activity at concentrations up to 10(-4) M, but stimulated beta-galactosidase expression at higher concentrations resulting in a U-shaped-like dose-response curve.

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“…Bee pollen is also a very important source of vitamins and polyphenolic compounds, particularly flavonoids [1,2]. Some classes of these substances, similarly to natural estrogens (phytoestrogens), are able to bind intracellular estrogen receptors (ERs) thus causing estrogenic/antiestrogenic effects [3,4]. In addition, they exhibit a broad spectrum of biological effects which can be beneficial or harmful for human health.…”

Section: Introductionmentioning

confidence: 99%

Antiestrogenic and antigenotoxic activity of bee pollen from Cystus incanus and Salix alba as evaluated by the yeast estrogen screen and the micronucleus assay in human lymphocytes

Pinto

Caciagli

Riccio

et al. 2010

European Journal of Medicinal Chemistry

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Assessment of estrogenic activity of flavonoids from Mediterranean plants using an in vitro short-term test

Cited by 26 publications

References 14 publications

Mediterranean Diet and Health: Food Effects on Gut Microbiota and Disease Control

Mediterranean Diet and Health: Food Effects on Gut Microbiota and Disease Control

Estradiol‐antagonistic activity of phenolic compounds from leguminous plants

Antiestrogenic and antigenotoxic activity of bee pollen from Cystus incanus and Salix alba as evaluated by the yeast estrogen screen and the micronucleus assay in human lymphocytes

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