Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd)

Abstract

Background Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its traditional claims, antioxidant and anti-proliferative activities were evaluated in the present study.MethodsEthanol extract (TCE) and subsequent petroleum ether (TCP), dichloromethane (TCD), n-Butanol (TCB) and aqueous (TCA) fractions of were prepared from stems of T cordifolia. Total phenolic, flavonoid content and anti-oxidant activity was assessed by different methods. Anti-proliferative activity was assessed in cervical carcinoma (HeLa) cell lines by MTT and SRB assay.ResultsEthanol extract and n-butanol fractions shown to be superior in their scavenging activity in all the tested methods. n-butanol fractions shown antioxidant activity with an IC50 of 14.81 ± 0.53, 29.48 ± 2.23, 58.20 ± 0.70 and 21.17 ± 1.19 μg/mL by DPPH, ABTS, Nitric oxide and iron chelating activities respectively. Anti-proliferative activity results demonstrates that the TCD and ethanol extract of T cordifolia exhibits potent cytotoxic effect against HeLa with an IC50 of 54.23 ± 0.94 μg/mL and 101.26 ± 1.42 μg/mL respectively by MTT assay; and with an IC50 of 48.91 ± 0.33 μg/mL and 87.93 ± 0.85 μg/mL respectively by SRB assay.ConclusionThe outcomes of the present study support the fact that T Cordifolia is a promising source of antioxidant agent and propose its further investigation. Moreover, dichloromethane fraction of T cordifolia shown to be the most potent anti-proliferative fraction and further mechanistic and phytochemical investigations are under way to identify the active principles.

Cordifolioside: potent inhibitor against Mpro of SARS-CoV-2 and immunomodulatory through human TGF-β and TNF-α

“…The antioxidant activity analyzed by ABTS method in different extracts were ranging from 119.61 to 172.77 µmTE/g, wherein the highest activity was found in the ethyl alcohol and the least in the water extract. A similar study by Polu et al ( 2017 ), reported antioxidant activity by ABTS method in T. cordifolia which was between 28.24 and 132.4 IC 50 µg/mL, with exception of petroleum ether extract with more than 1000 IC 50 µg/mL. The results for the FRAP method of antioxidant activity in different extracts were found to be between 0.78 and 1.15 mmol Fe(II)/g, wherein ethyl alcohol and methanol depicted the highest content in comparison to acetone and water extracts.…”

“…Whereas when compared among solvents, ethyl alcohol and methanol had a higher content of TFC compared with acetone and water (Table 1 ). Hydro-alcoholic extract of T. cordifolia plant was reported for TFC with 18.91 mg QE/g, whereas TFC of T. cordifolia stem extracts in ethyl alcohol, petroleum ether, dichloromethane, n -butanol, and aqueous solvents were reported between 0.17 and 0.98 mg QE/g (Polu et al 2017 ).…”

“…The DPPH method displayed antioxidant activity, which was ranging from 109.34 to 162.11%. Another study reported DPPH activity for different solvent extracts of T. cordifolia as IC 50 ranging from 14.18 to 183.47 µg/mL (Polu et al 2017 ). The antioxidant activity analyzed by ABTS method in different extracts were ranging from 119.61 to 172.77 µmTE/g, wherein the highest activity was found in the ethyl alcohol and the least in the water extract.…”

Untitled

“…Ethanolic extract of Albizia procera showed strong scavenging activity against free radical compared to various standards 58 . These in-vitro assays indicate that this plant extract is a better source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses [59][60][61][62] .…”

Assessments of Antioxidant Content and the Anti-Carcinogenic Effect of Extracts of Solanum Rostratum Dunal in Human Cancer Cells

“…DPPH ï is a stable free radical resulting from the delocalization of the spare electron over the molecule. When DPPH ï is mixed with a substance donor of the hydrogen atom, its reduced form is produced (DPPH-H), in which it loses the violet color and forms a yellow color (17,18). On the other hand, the ABTS ï+ assay involves the direct production of the blue-green ABTS ï+ through the reaction between ABTS ï+ and potassium persulfate.…”