Assessment of Freezability and Functional Integrity of Dromedary Camel Spermatozoa Harvested from Caput, Corpus and Cauda Epididymides

Abstract

The present study aimed to assess the freezability and functional integrity of dromedary camel spermatozoa harvested from three epididymal regions. Twenty five epididymes were obtained from slaughtered adult camels. The cauda, corpus and caput epididymides were isolated, incised and rinsed for obtaining the sperm rich fluid. Portion of this fluid was processed for cryopreservation. Fresh and frozen-thawed spermatozoa collected from different epididymal regions were evaluated for motility, livability, morphological abnormalities, membrane and acrosomal integrities as well as mitochondrial activity. Also, in vitro fertilization using camel mature oocytes and measuring DNA integrity using Comet assay were performed for these spermatozoa. The results showed that, there were no significant differences in livability among spermatozoa freshly collected from cauda, corpus and caput epididymides (81.16 ± 1.43, 80.20 ± 0.90 and 76.76 ± 1.95%, respectively). Total sperm motility increased dramatically from the caput (22.60 ± 0.96%) to the cauda (67.92 ± 1.14%) of the epididymis. Viability index of cauda frozen-thawed spermatozoa (96.50 ± 2.36%) was significantly higher than those of corpus (53.20 ± 3.11%) and caput epididymides (12.10 ± 1.10%). Fresh and frozen thawed spermatozoa of the cauda epididymides had significantly higher percentages of membrane integrity, cytoplasmic droplets and MTT reduction rate than the corresponding parameters of corpus and caput spermatozoa. Fresh and frozen thawed spermatozoa of the cauda epididymides had significantly higher fertilization rates (50.88 ± 1.10 and 38.64 ± 0.77%, respectively) than those of corpus (36.92 ± 0.79 and 22.16 ± 0.79%, respectively) and caput epididymides (12.48 ± 1.09 and 4.36 ± 0.59%, respectively). Only oocytes fertilized with fresh and frozen-thawed cauda epididymal spermatozoa developed to blastocysts (10.92 ± 0.52 and 8.12 ± 0.81%, respectively). The percentage of fresh cauda epididymal spermatozoa with non-fragmented DNA was higher than those of corpus and caput of epididymis (90.88 ± 1.55 vs. 78.28 ± 0.72 and 76.24 ± 1.02%, respectively). In conclusion, obtaining spermatozoa of good quality and freezability from dromedary camel cauda epididymides is possible, and these fresh and frozen-thawed spermatozoa may have the potential uses in IVF and AI for improving breeding potentials in this species.

Retrograde flushing collection and freezing of dromedary camel epididymal spermatozoa with seminal plasma

“…In previous studies, with subjective evaluation of ES postthaw motility, Abdoon et al (2013) observed higher postthaw total motility (47 ± 5%) by using Ovixcell ® . By using Shotor diluent, El-Badry et al (2015) obtained total and progressive motility values of 47.4 ± 1.3% and 32.8 ± 1.3%, respectively. More recently, post-thaw CASA evaluation of ejaculated spermatozoa revealed total and progressive motility values of 38.6 ± 0.8% and 10 ± 0.7% for spermatozoa diluted with Triladyl ® (Swelum et al, 2019), andMalo et al (2019b) observed total motility values ranging from 33% to 46% and progressive motility from 8 to 18.5% for spermatozoa frozen with Green Buffer ® .…”

In Vitro Fertilizability of Oocytes Using Fresh, Frozen and Epididymal Spermatozoa from Crossbred Bulls in the Tropics

“…The effect of seminal plasma on epididymal spermatozoa has been suggested as being due to heparin-binding proteins potentiating the ability of epididymal spermatozoa to be capacitated by heparin and to undergo acrosomal exocytosis at frequencies similar to those of epididymal sperm. Many scientists recommended the use of frozen cauda epididymal spermatozoa for mass embryo production of unknown genetic value (Katska et al, 1996;El-Badry et al, 2015).…”

Characteristics of Ultrasound and Magnetic Resonance Imaging of Normal Testes and Epididymis Besides Angiography of Testicular Artery in Dromedary Camel

“…Heads and tails of the epididymides were measured using a Vernier caliper. Testicular volume was calculated as: volume = [length × width × height] ×0.5236 ( 24 , 25 ).…”