Assessment of genetic diversity among 125 cultivars of chickpea(Cicer arietinum L.) of Indian origin using ISSR markers

Aggarwal, Himanshu; Rao, A. Mohan; Kumar, Anil; Singh, Jasbir; Rana, J. S.; Naik, Pradeep Kumar; Chhokar, Vinod

doi:10.3906/bot-1401-80

Cited by 13 publications

(14 citation statements)

References 37 publications

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“…Percentage of polymorphic bands (PPB) varied between 17 -43%, the highest value was obtained by IS 11, while UBC 818 with sequence of (CA)8G produced the lowest polymorphism. The average polymorphism in the whole collection was 30.2%, which is low than the value described by Aggarwal et al (2015). Comparable to this study Sudupak (2004) and Iruela et al (2002) detected a low level of variation within C. arietinum using ISSR markers.…”

Section: Resultsmentioning

confidence: 63%

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Babayeva

Nasibova

Akparov

et al. 2018

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Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.

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Section: Resultsmentioning

confidence: 63%

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Babayeva

Nasibova

Akparov

et al. 2018

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“…Herein DNA based molecular markers give more clear results than phenotypic markers which present variability depending on environmental factors (Virk et al, 1995;Serret et al, 1997). Likewise, classical and molecular characterisation of agronomic characters in chickpea genotypes concerning biotic, abiotic tolerance/resistance and food items are emphasized by numerous studies (Talebi et al, 2008;Aggarwal et al, 2015). Koinain et al (2016) and Kumar et al (2017) determined genetic variability among wild and cultivated Cicer spp.…”

Section: Introductionmentioning

confidence: 99%

“…Sudupak et al (2002) used RAPD markers to evaluate genetic similarity among perennial and annual wild Cicer spp collected from Turkey and defined two different groups within annual species. They Table 1: Sequences of RAPD and ISSR primers (Talebi et al, 2008;Aggarwal et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic and biochemical properties of Cicer spp reveal distinction between wild and cultivated chickpea genotypes

Kafadar

Özkan

Can

et al. 2018

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Chickpea (Cicer arietinum L.) is the third most important legumes after beans and peas in the world. Turkey is one of the main chickpea producing countries but the yield is not within the expected quantity considering cultivated area. Breeding chickpea varieties requires well-characterized wild Cicer spp progenitors including C. reticulatum, C. echinospermum, C. bijugum and C. pinnatifidum that exhibit sympatric distribution in the Southeastern Anatolia region of Turkey. This study contributes to the identification of similarities and differences among wild and cultivated Cicer spp. ecotypes using DNA marker systems and biochemical parameters. RAPD and ISSR markers revealed four groups and separated C pinnatifidum ecotypes as a single population. Wild Cicer spp and cultivated chickpea species exhibited significant variations for their oil, protein, starch, cellulose, fructose and sucrose content. Furthermore, the cultivated chickpea genotypes had higher content of fat, starch and sucrose than those of the wild progenitors whereas wild Cicer spp ecotypes possessed higher values for cellulose, fructose and glucose than cultivated chickpea genotypes.

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“…Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License type (FAO, 2013). The cultivated species is found in West Asia and North Africa covering Turkey in the north to Ethiopia in the south, and Pakistan in the east to Morocco in the west (Aggarwal et al, 2015). Chickpea is valued for its high dietary protein content, ability to fix atmospheric nitrogen and the absence of major anti-nutritional factors (Gautam et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of chickpea (Cicer arietinum L.) cultivars from Ethiopia by using ISSR markers

Gubae¹,

Petros²

2018

J. Agric. Biotech. Sustain. Dev.

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Inter Simple Sequence Repeat (ISSR) markers were employed to reveal genetic diversity and relatedness among 27 chickpea (Cicer arietinum L.) cultivars in Ethiopia. Four di-nucleotide repeat primers amplified 24 clear and reproducible bands of which 22 were polymorphic (91.67%). The genetic variation among 27 chickpea cultivars including 12 kabuli and 15 desi verities is high; in which desi type exhibited a genetic diversity of 75% with Shannon index of 0.47, while the kabuli type chickpea had 91.67% genetic diversity and Shannon index of 0.50. Unweighted Pair Group of Arithmetic mean (UPGMA) dendrogram and NJ trees with Jaccard's similarity coefficient showed three major clusters. This was also recovered by 3D principal coordinates analysis, although some cultivars were intermixed. Analysis of molecular variance (AMOVA) demonstrated highly significant (p < 0.001) genetic diversity within cultivars (97.71%) than among cultivars (2.29%). The distinct cultivars (Aererti, Tejie, Fetenech and Maryie) can serve as parents for future genetic resources conservation and Chickpea breeding program in Ethiopia.

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Assessment of genetic diversity among 125 cultivars of chickpea(Cicer arietinum L.) of Indian origin using ISSR markers

Cited by 13 publications

References 37 publications

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Genetic and biochemical properties of Cicer spp reveal distinction between wild and cultivated chickpea genotypes

Genetic diversity of chickpea (Cicer arietinum L.) cultivars from Ethiopia by using ISSR markers

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