Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11
RAPD primers. In the study RAPD primers detected more polymorphism (98%)
than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR
and 0.85 for RAPD) for each of these marker systems. Cluster analysis
performed from both separate and combined data of RAPD and ISSR markers
using SPSS software package. Jaccard?s similarity coefficient for 62
chickpea genotypes was 0.65. Cluster analyses based on combined data
generated a dendrogram that separated genotypes into 11 clusters. Four
clusters contained only one genotype showing the genetic uniqueness of these
accessions. The studied chickpea collection has been proved to constitute a
rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing
between distantly related genotypes is expected to yield more vigorous
plants constituting much of the different traits contained in the two
parental lines.
Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.
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