Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers

Hasanova, Saida; Akparov, Zeynal; Mammadov, Alamdar; Amirov, Litfer; Babayeva, Sevda; Nasibova, J. A.; Mukhtarova, Z.M.; Shikhaliyeva, Kamila; İzzatullayeva, Vusala; Abbasov, Mehraj

doi:10.2298/gensr1702415h

Cited by 12 publications

(11 citation statements)

References 13 publications

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“…Among all studied ISSR and RAPD loci the RAPD primer OPJ20 was characterized with the highest number of total bands, gene diversity index, PIC and MRP. In some other researches, a more usefulness of RAPD markers in compare with ISSRs has been reported (Hasanova et al, 2017). On contrary, in the comparative marker analysis among chickpea accessions Bhagyawant et al (2015) have revealed a better polymorphism with ISSR markers than with RAPDs.…”

Section: Resultsmentioning

confidence: 95%

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Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Babayeva

Nasibova

Akparov

et al. 2018

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Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.

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Section: Resultsmentioning

confidence: 95%

“…Only few works have been published on molecular diversity of local chickpea landraces of Azerbaijan (Hasanova et al, 2017). In this study ISSR and RAPD markers were used to assess diversity and relationship among local and introduced chickpea genotypes.…”

Section: Resultsmentioning

confidence: 99%

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Babayeva

Nasibova

Akparov

et al. 2018

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“…It is a dominant multi-locus marker system based on PCR amplification of regions in the genome flanked by microsatellite sequences. Higher reproducibility compared to RAPDs and costs less in terms of time and money compared to AFLP make ISSR ideal genetic marker for genetic diversity IZZATULLAYEVA et al, 2014;HASANOVA et al, 2017) and DNA fingerprinting (BHAGYAWANT and SRIVASTAVA, 2008;LIU et al, 2012) studies. ISSR markers are increasingly applied and detected a sufficient degree of polymorphism in a wide range of crops such as chickpea, barley, faba bean, wheat etc.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers

et al. 2018

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Lentil (Lens culinaris Medik.) is an annual, cool-season grain legume playing an important role in human and animal nutrition, as soil fertility maintenance. National lentil improvement program in Azerbaijan is currently focused on extending the genetic base of the lentil collection through the introduction of new breeding lines from ICARDA and involving them into breeding. The present study was aimed to evaluate the performance of lentil collection, mainly comprised of ICARDA-derived breeding lines for yield traits under Azerbaijan condition and assess genetic diversity among them using inter simple sequence repeats (ISSR) markers. Many breeding lines of ICARDA exhibited agromorphological performance superior to those of the local improved varieties. Our studies confirmed that the genetic base of the studied lentil collection is quite above board. A total of 71 bands were generated using 7 ISSR primers in 47 lentil genotypes, of which 62 were polymorphic. Genetic diversity values varied from 0.61 (UBC 848) to 0.95 (UBC 835), with a mean of 0.81. ISSR dendrogram was able to clearly distinguish all lentil accessions. Clear tendention was observed on clustering of genotypes according to their pedigree or origin with few exeptions. The results obtained from the Principal Coordinate Analysis were consistent with the results of cluster analysis, with minor differences. Breeding lines with high agronomic performance and sufficient genetic distance from this study can be used as appropriate parents to get more heterotic recombinants. This will accelerate the creation of new varieties well adapted to eco-geographic condition of Azerbaijan with stable and high yield.

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“…Different types of DNA markers have been applied in evaluation of genetic diversity of different plants, considering also the effects of the plant growing environment and developmental stage (Nadeem et al, 2018). DNA markers are genes or intergenic sequences used to define and interpret the sequentional genetic variability in organisms (Govindaraj et al, 2015;Hasanova et al, 2017). Some of them are suitable for use without any background information about the species sequences, such as ivy.…”

Section: Introductionmentioning

confidence: 99%

Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

et al. 2019

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Žiarovská, J., Ražná, K., Fernandéz, E.C., Bošeľová, D., Kyseľ, M., 2019. Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L. Folia Oecologica, 46: 30-36.Inter primer binding site (iPBS) polymorphism was investigated for common ivy (Hedera helix, L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in Hedera helix. Natural and planted European populations of ivy were analysed using an iPBS marker 5´ACCTGGCGTGCCA3´ with a total number of 238 fragments generated. Of these, 86% were polymorphic. There were determined certain attributes of this marker such as the diversity index (DI) and polymorphism information content (PIC). The value of the diversity index was 0.79 and the polymorphic information index was 0.78. The proportion of polymorphisms of the individual amplified loci ranged from 0.32% to 6.98%. Cluster analysis was performed to determine the relationships among the European ivy populations where the distribution in the dendrogram under the habitat specifity was found for the used iPBS marker. We concluded that iPBS was very efficient in analysing the genetic diversity in Hedera helix, L. and that this marker can serve as a suitable tool to find genomically specific fingerprints relevant to the factors influencing the distribution of genetic variation.

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Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers

Cited by 12 publications

References 13 publications

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers

Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

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