Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

Abstract

Žiarovská, J., Ražná, K., Fernandéz, E.C., Bošeľová, D., Kyseľ, M., 2019. Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L. Folia Oecologica, 46: 30-36.Inter primer binding site (iPBS) polymorphism was investigated for common ivy (Hedera helix, L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in Hedera helix. Natural and planted European populations of ivy were analysed using an iPBS marker 5´ACCTGGCGTGCCA3´ with a total number of 238 fragments generated. Of these, 86% were polymorphic. There were determined certain attributes of this marker such as the diversity index (DI) and polymorphism information content (PIC). The value of the diversity index was 0.79 and the polymorphic information index was 0.78. The proportion of polymorphisms of the individual amplified loci ranged from 0.32% to 6.98%. Cluster analysis was performed to determine the relationships among the European ivy populations where the distribution in the dendrogram under the habitat specifity was found for the used iPBS marker. We concluded that iPBS was very efficient in analysing the genetic diversity in Hedera helix, L. and that this marker can serve as a suitable tool to find genomically specific fingerprints relevant to the factors influencing the distribution of genetic variation.

ISSR markers and morphometry determine genetic diversity and population structure in Hedera helix L.

“…Previously, different molecular methods have revealed the high genetic diversity, genetic variation, polymorphism in the common ivy plant. Molecular markers coupled with statistical analyses have deciphered the population structure in H. helix populations (Boselova & Ziarovska 2016;Li et al 2021;Ziarovska et al 2019). The current study methods and findings are novel due to the application of morphometric methods to disentangle the genetic diversity and population structure in the H. helix population.…”