Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers

doi:10.12692/ijb/12.4.172-180

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…The outcomes from STRUCTURE analyses agreed regarding the relatedness of genomic information. This observation is also in concordant with various genetic structure and diversity studies were conducted on Thai Musa genetic resources and associated according to the genotypes of cultivars (Rotchanapreeda et al 2016;Zozimo et al 2018;Wanvisait et al 2019;Boonsrangsom et al 2020;Premjet et al 2022). The results proposed that SCoT markers were effective in genomic discrimination.…”

Section: Genetic Diversity and Cluster Analysissupporting

confidence: 84%

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

NITIWORAKARN,

PHAE-NGAM,

VANIJAJIVA

2023

Biodiversitas

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Abstract. Nitiworakarn S, Phae-Ngam W, Vanijajiva O. 2023. Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers. Biodiversitas 24: 4060-4068. Musa cultivars are mostly obtained from crossing between wild diploid subspecies of M. acuminata (A genome) and M. balbisiana (B genome), and they reveal numerous levels of ploidy and genomic constitution. The present study, for the first time, investigated the genetic diversity of 90 Musa cultivars from 17 localities of Chai Badan district in Lopburi Province, Thailand, using start codon targeted (SCoT) markers. The SCoT technique has shown very high repeatability for characterizing Musa cultivars. The result indicated that DNA fingerprints from 30 SCoT primers generated 244 amplicons, among which 238 bands (97.54%) were polymorphic, with a mean of 7.93 polymorphic bands per primer. The average polymorphism information content (PIC) was high, ranging from 0.345 to 0.483, with an average of 0.432. Genetic diversity and its partitioning parameters were calculated and demonstrated that present Musa cultivars maintain relatively high genetic diversity. The unweighted pair-group method with an arithmetic cluster analysis, principal coordinate analysis and STRUCTURE analysis results were the same and showed the clear division of the genotypes into two distinct clusters, which were cluster-I consisted of only the AA genomes and cluster-II comprised of ABB and BB genomes. In conclusion, SCoT markers obtained here showed their manipulation in genetic diversity and the relationship of cultivated Musa, which would be practical for genetic preservation and continuing breeding programs in Thailand and other regions.

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Section: Genetic Diversity and Cluster Analysissupporting

confidence: 84%

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

NITIWORAKARN,

PHAE-NGAM,

VANIJAJIVA

2023

Biodiversitas

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“…Characterization is suggested using morphotaxonomy, cytology, and molecular genotyping, including of nutritional traits [ 9 ]. Molecular markers, including random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), simple sequence repeat (ISSR), and sequence-related amplified polymorphism (SRAP) markers, have been employed to examine genetic diversity among banana accessions and species [ 2 , 10 , 11 , 12 ]. SRAP markers have been used to determine the relationship of a cross between Musa (ABB) and two wild cultivars (AA and BB) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Characterizations of Musa (ABB) ‘Mali-Ong’ in Thailand

Premjet

Boonsrangsom

Sujipuli

et al. 2022

Biology

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Musa (ABB) ‘Mali-Ong’ is an economically important banana cultivar in Thailand. We morphologically and molecularly characterized ‘Nam Wa Mali-Ong’. Leaf blade width was the only statistically different morphological character among the clones. To determine genetic variation, nine ‘Nam Wa Mali-Ong’ clones were compared with 10 samples of Musa ABB, AA, and BB cultivars by fingerprinting using seven pairs of sequence-related amplified polymorphism (SRAP) and eight inter simple sequence repeat (ISSR) markers. The SRAP and ISSR primers generated 65 and 76 amplicons, respectively, of which 57 (87.7%) and 62 (81.6%) amplicons, respectively, were polymorphic; the polymorphic information content was 0.28–0.49. The SRAP data revealed two distinct groups: Group I, comprising two subgroups (one including all ABB samples and the other containing the BB genome accessions), and Group II, comprising the AA genome accessions. The ISSR data revealed two groups: Group I, which incorporated the AA (Hom Champa) genome, and Group II, consisting of two subgroups: Subgroup A, comprising only the AA (Hom Chan) accessions, and subgroup B, comprising all the ABB accessions and wild banana M. balbisiana (BB genome). The ‘Nam Wa Mali-Ong’ samples clustered together, regardless of the markers used. SRAP and ISSR markers will be useful for germplasm evaluation and future Musa (ABB) improvements.

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Physicochemical and prebiotic properties of resistant starch from Musa sapientum Linn., ABB group, cv. Kluai Namwa Luang

Jaiturong

Laosirisathian

Sirithunyalug

et al. 2020

Heliyon

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Resistant starch (RS), a current health trend, can be obtained from various natural sources. Musa sapientum Linn., ABB group, cv. Kluai Namwa Luang is a good source of RS. This is the first study to investigate the physicochemical properties, RS contents, and prebiotic properties of unpeeled raw banana powder (URB), peeled raw banana powder (PRB), and banana starch (BS) from Kluai Namwa Luang. Their physicochemical properties were characterized by scanning electron microscope, differential scanning calorimeter, and X-ray diffractometer. The RS contents were determined using the Megazyme Resistant Starch Assay Kit. The prebiotic properties are reported as a prebiotic index (PI). The particle morphology of URB, PRB, and BS granules showed a smooth surface with irregular size and shape. Their gelatinization temperatures were 74–78 °C. All samples exhibited typical B-type diffraction patterns. URB contained the highest dietary fiber (9.7 ± 0.2 g per 100 g of dried sample), whereas BS contained the highest RS content (74.1 ± 0.1 g per 100 g of dried sample). Both URB and BS possessed excellent probiotic growth promotion, prebiotic properties with PI values comparable to the commercial inulin, and were highly resistant to digestive enzymes. Therefore, BS from Kluai Namwa Luang is suggested as functional nutrient in health promotion products.

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Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers

Cited by 4 publications

References 5 publications

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

Morphological and Molecular Characterizations of Musa (ABB) ‘Mali-Ong’ in Thailand

Physicochemical and prebiotic properties of resistant starch from Musa sapientum Linn., ABB group, cv. Kluai Namwa Luang

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