Assessment of genetic diversity among and within Achillea species using amplified fragment length polymorphism (AFLP)

Rahimmalek, Mehdi; Tabatabaei, Badraldin Ebrahim Sayed; Arzani, Ahmad; Etemadi, Nematolah

doi:10.1016/j.bse.2009.06.002

Cited by 26 publications

(18 citation statements)

References 22 publications

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“…Af1 and Af2 accessions possessed only one chromosome pair with a satellite, while Af3 accession had three chromosome pairs with satellites. These findings are in agreement with those of Rahimmalek et al (2009) who found same discrimination using AFLP analysis on the same accessions. It should be noted that Af3 was collected from the Northwestern regions of the country at high elevations.…”

Section: A Filipendulinasupporting

confidence: 93%

“…Am5 accession was the only accession possessing satellite at two chromosome pairs. The results of amplified fragment length polymorphism (AFLP) analysis using the same accessions by Rahimmalek et al (2009) showed that Am5 (Am40 in Rahimmalek et al 2009) separated from other genotypes in the A. millefolium cluster. Therefore, it seems that Am5 has different cyto-taxonomical characteristics and further consideration of the classification of this accession is warranted.…”

Section: Achillea Millefoliummentioning

confidence: 99%

“…Yarrow (Achillea L.) is one of the youngest evolutionary genera of the Asteraceae family, which is spread throughout the world (Rahimmalek et al 2009). More than 100 species have been recognized in Achillea genus (Rechinger 1963).…”

Section: Introductionmentioning

confidence: 99%

“…Polyploid taxa have originated in many clades independently and include 4x, 6x and 8x species (Sheidai et al 2009). The genus exhibits great ecological amplitude, ranging from deserts to water-logged habitats and from sea coasts to the high mountains (Ehrendorfer & Guo 2006;Rahimmalek et al 2009). Several widespread Achillea species show extremely high morphological variability (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Karyotype analysis and new chromosome number reports in Achillea species

et al. 2012

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Cytological analyses were performed in nineteen accessions belonging to seven Achillea species. The results indicate that two species, A. filipendulina and A. tenuifolia, are diploid (2n = 2x = 18) and three species, A. bieberstinii, A. pachycephala, and A. aucheri are tetraploid (2n = 4x = 36). For two latter species, the chromosome numbers are new. Two ploidy levels of 2n = 6x = 54 and 2n = 8x = 72 in A. millefolium and two ploidy levels of 2n = 2x = 18 and 2n = 4x = 36 in A. santolina species were found. Cluster analysis based on chromosomal characteristics and karyotype asymmetry indices clustered the studied genotypes into three major groups. The first group included the diploid species (A. filipendulina, A. santolina and A. tenufolia), while the second group comprised tetraploid species (A. santolina, A. aucheri and A. pachycephala). A. millefolium (hexaploid, octaploid) and A. bieberstinii (tetraploid) were classified in the third group. Total form percentage (TF%) in groups 1, 2 and 3 were 42.03, 42.15 and 41.08, respectively. Group 1 possessed the highest average of symmetry index (S% = 70.8). Stebbins classification method grouped all accessions in class A. Moreover, group 1 (diploid level species), had the most symmetric karyotype from point of view of centromere and chromosome length. Therefore, it can be concluded that the genotypes belonging to group 1 are the earliest evolutionary form.

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Section: A Filipendulinasupporting

confidence: 93%

Section: Achillea Millefoliummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Karyotype analysis and new chromosome number reports in Achillea species

et al. 2012

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“…Rahimmalek et al (2009) have studied inter-and intra-genetic diversity of Achillea species using amplified fragment length polymorphism markers. Gharibi et al (2011) applied ISSR markers to the study of genetic variation in two sub-species of Achillea millefolium in Iran.…”

Section: Introductionmentioning

confidence: 99%

Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

Ebrahimi¹,

Farajpour²,

Rahimmalek³

2012

Genet. Mol. Res.

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ABSTRACT. Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 187 amplified fragments, of which 159 were polymorphic. The similarity coefficient among Achillea tenuifolia accessions ranged from 61 to 86%, and from 40 to 84% among A. santolina accessions. A low similarity was observed between these two species (mean similarity = 0.36%). This low similarity is consistent with their geographical distribution. According to the results of cluster and PCA analyses, the two species completely separated from each other. These markers will aid in the identification of elite genotypes for domestication and breeding programs.

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A population genetics perspective on the evolutionary histories of three clonal, endemic, and dominant grass species of the Qinghai–Tibet Plateau: Orinus (Poaceae)

Liu

Harris

Gao

et al. 2019

Ecology and Evolution

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We performed analyses of amplified fragment length polymorphism (AFLP) in order to characterize the evolutionary history of Orinus according to its population genetic structure, as well as to investigate putative hybrid origins of O. intermedius and to provide additional insights into relationships among species. The genus Orinus comprises three clonal grasses that are dominant species within xeric alpine grasslands of the Qinghai–Tibet Plateau (QTP). Here, we used eight selectively obtained primer pairs of EcoRI/MseI to perform amplifications in 231 individuals of Orinus representing 48 populations and all three species. We compared our resulting data to genetic models of hybridization using a Bayesian algorithm within NewHybrids software. We determined that genetic variation in Orinus was 56.65% within populations while the among‐species component was 30.04% using standard population genetics statistics. Nevertheless, we detected that species of Orinus were clustered into three highly distinct genetic groups corresponding to classic species identities. Our results suggest that there is some introgression among species. Thus, we tested explicit models of hybridization using a Bayesian approach within NewHybrids software. However, O. intermedius likely derives from a common ancestor with O. kokonoricus and is probably not the result of hybrid speciation between O. kokonoricus and O. thoroldii. We suspect that recent isolation of species of Orinus in allopatry via vicariance may explain the patterns in diversity that we observed, and this is corroborated by a Mantel test that showed significant positive correlation between geographic and genetic distance (r = 0.05, p < 0.05). Recent isolation may explain why Orinus differs from many other clonal species by exhibiting the highest diversity within populations rather than among them.

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Assessment of genetic diversity among and within Achillea species using amplified fragment length polymorphism (AFLP)

Cited by 26 publications

References 22 publications

Karyotype analysis and new chromosome number reports in Achillea species

Karyotype analysis and new chromosome number reports in Achillea species

Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

A population genetics perspective on the evolutionary histories of three clonal, endemic, and dominant grass species of the Qinghai–Tibet Plateau: Orinus (Poaceae)

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