Assessment of Genetic Diversity and its Usefulness for Varietal Identification in Indian Elite Varieties of Wheat (T. aestivum L. em thell.) Using RAPD Markers

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“…The average number of polymorphic fragments per primer among the 36 cultivars of six major cereal crops like rice, wheat, maize, barley, pearl millet and sorghum was 5.35. This percentage of polymorphic bands was similar to that observed in the study [13][14][15][16][17] . It is generally reported that polymorphism between cultivars can arise through nucleotide changes that prevent amplification by introducing a mismatch at one priming site; deletion of a priming site; insertions that render priming site too distant to support amplification and insertions or deletions that change the size of the amplified product [22] .…”

“…RAPD markers are considered to be unbiased and neutral markers for genetic mapping applications [9] , in population genetics [10] , taxonomy [11] as well as for genetic diagnostics. RAPD has been used for classification and assessing diversity and relatedness of six major cereal crops genotypes by several groups [12][13][14][15][16][17] . This study aimed to use RAPD markers to evaluate the genetic variation within a collection of improved six major cereal crops like rice, wheat, maize, barley, pearl millet and sorghum varieties [18] and to reveal genetic relationships among them for future use in selection, hybridization, biodiversity assessment and conservation of diverse gene pools.…”