Assessment of genetic diversity, genetic relationship and bottleneck using microsatellites in some native Turkish goat breeds

Ağaoğlu, Özgecan Korkmaz; Ertuğrul, Okan

doi:10.1016/j.smallrumres.2011.12.005

Cited by 34 publications

(16 citation statements)

References 24 publications

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“…The mean number of alleles/locus/breed found in this study (between 10.33 and 11.33) were similar to those found by Bozkaya et al 49 , Korkmaz-Ağaoğlu and Ertuğrul 50 and higher than those reported by Behl et al 35 , Saitbekova et al 38 , Agha et al 39 , Barker et al 45 , Li et al 48 , Gour et al 51 , Jandurova et al…”

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confidence: 90%

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Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Gürler¹,

Bozkaya²

2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the genetic variability of three goat populations raised in the Southeastern region of Turkey by using microsatellite loci defined in gazelle (Gazella granti). DNA was isolated from 120 blood samples collected from goats raised in Sanliurfa (n = 40), Kilis (n = 40) and Siirt (n = 40) provinces. Eight microsatellite loci (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) were used for amplification with polymerase chain reaction (PCR). Fragment lengths of the amplifed loci were analysed using a capillary electrophoresis system. Six of the eight loci (75%) were successfully amplified and four (66.7%) of them were polymorphic. Three of the polymorphic loci (33HDZ496, 33HDZ749 ve 33HDZ974) were used for estimating genetic parameters. A total of 40 alleles were detected. Number of alleles and polymorphism information contents (PIC) for the loci 33HDZ496, 33HDZ749 and 33HDZ974 Keywords: Goat, Microsatellite, Genetic diversity Güney Doğu Anadolu Bölgesi'nde Yetiştirilen Üç Keçi Populasyonunda Genetik Çeşitlilik ÖzetBu çalışma bir ceylan türünde (Gazella granti) tanımlanmış sekiz mikrosatelit lokusunu kullanarak Güneydoğu Anadolu Bölgesinde yetiştirilen üç keçi populasyonunu genetik çeşitlilik yönünden değerlendirmek amacıyla yapılmıştır. Bu amaçla, Şanlıurfa (n = 40), Kilis (n = 40) ve Siirt (n = 40) illerinde yetiştirilen üç keçi populasyonundan toplam 120 adet kan örneği alınmış ve bu örneklerden DNA izole edilmiştir. İzole edilen DNA'lar sekiz mikrosatelit lokusuna (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) ait primerler kullanılarak PCR ile çoğaltılmış ve PCR ürünleri kapillar elektroforeze tabi tutularak fragman uzunluğu analizi gerçekleştirilmiştir. Sekiz lokustan altısı (%75) PCR ile amplifiye olmuş ve bunlardan dördü (%66.7) polimorfik bulunmuştur. Polimorfik lokusların üçü (33HDZ496, 33HDZ749 ve 33HDZ974) genetik parametrelerin hesaplanmasında kullanılmıştır. Çalışmada toplam 40 allel tespit edilmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için sırasıyla 13, 15 ve 12 allel gözlenmiş olup, herbir lokus için hesaplanan polimorfizm bilgi içeriği (PIC) aynı sırayla 0.836, 0.821 ve 0.817 olarak belirlenmiştir. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama allel sayıları sırasıyla 10.33±1.15, 10.67±2.08 ve 11.33±0.58, etkili allel sayıları ise yine aynı sıra ile 6.131±1.42, 5.823±0.23 ve 5.870±0.75 olarak bulunmuştur. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama gözlenen heterozigotluk oranları sırasıyla 0.827±0.083, 0.808±0.044 ve 0.895±0.069, beklenen heterozigotluk oranları ise yine aynı sıra ile 0.843±0.043, 0.841±0.007 ve 0.838±0.023 olarak belirlenmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için hesaplanan FIS değerleri sırasıyla 0.0164, 0.0245 ve 0.0240 olarak bulunmuştur. Tüm populasyonlar için hesaplanan FST değeri (0.009) incelenen populasyonda gözlenen genetik çeşitliliğin %99'unun bireyler arasındaki farklılıktan kaynaklandığını göstermiştir. Çalışma sonuçları incelenen ke...

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supporting

confidence: 90%

“…Therefore the higher values of heterozygosities obtained in this study might be due to loci used. The F IS values estimated for Sanliurfa (0.019), Kilis (0.040) and Siirt (-0.068) populations were similar to those reported by Korkmaz-Agaoglu and Ertugrul 50 for some Turkish goat breeds.…”

supporting

confidence: 86%

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Gürler¹,

Bozkaya²

2013

Kafkas Univ Vet Fak Derg

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“…Yayladag and Shami goats are raised in the southern part of Turkey, mainly in Hatay Province and close to the Syrian border. Phenotypic, population, and geographical properties of these breeds were described in detail elsewhere [3, 4]. …”

Section: Introductionmentioning

confidence: 99%

“…It was reported that microsatellites had been most frequently used in genetic characterization studies conducted in Asian and African countries [12]. In Turkey, genetic diversity studies with microsatellites had been conducted in goat [13], cattle [14, 15], and horse [16] populations.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Eight Domestic Goat Populations Raised in Turkey

Bulut

Kurar

Özşensoy

et al. 2016

BioMed Research International

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The objective of this study was to determine the intra- and intergenetic diversities of eight different goat populations in Turkey including Hair, Angora, Kilis, Yayladag, Shami, Honamli, Saanen, and Alpine. A total of 244 DNA samples were genotyped using 11 microsatellites loci. The genetic differentiation between breeds was considerable as a result of the statistically significant (P < 0.001) pairwise F ST values of each pair of breeds. Exceptionally, F ST values calculated for Honamli and Hair breeds were statistically nonsignificant (P > 0.05). Heterozygosity values ranged between 0.62 and 0.73. According to the structure and assignment test, Angora and Yayladag goats were assigned to the breed they belong to, while other breeds were assigned to two or more different groups. Because this study for the first time presented genetic data on the Yayladag goat, results of structure analysis and assigned test suggest that further analyses are needed using additional and different molecular markers.

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“…Turkey has rich domestic animal genetic resources and genetic diversity as it is located at the crossroads of Europe, Asia, and Africa (1). Native goat breeds are important genetic resources in livestock due to their socioeconomic value.…”

Section: Introductionmentioning

confidence: 99%