In vivo embryo production in some native goat breeds in Turkey

Ağaoğlu, Ali Reha; Karakaş, Kübra; Kaymaz, Mustafa

doi:10.3906/vet-1311-51

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…The progesterone source is removed from the vagina on the 11th day. GnRH injection is administered 24 hours after removal of progesterone source from the vagina and donors are allowed to mate with fertile males at 12 hours after GnRH administration (Thibier and Guerin 2000;Menchaca et al 2007;Gibbons and Cueto 2011;Agaoglu et al 2014;Karakas 2015). In fact, traditional superovulation protocols were first designed in 1980s.…”

Section: Synchronization and Superovulation Treatmentsmentioning

confidence: 99%

In Vivo Embryo Production in Sheep and Goats

2022

Int J Vet Sci

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Section: Synchronization and Superovulation Treatmentsmentioning

confidence: 99%

In Vivo Embryo Production in Sheep and Goats

2022

Int J Vet Sci

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“…Hayvan materyali: Projenin hayvan materyalini sağ-lıklı ve fertilitesi bilinen, en az bir defa doğum yapmış, 2- Embriyoların toplanması: Teke katımından sonraki 7. günde uterus'lar laparotomi yöntemiyle yıkanarak embriyolar toplandı (1,28). Keçiler 0.22 mg/kg dozunda xylazin (im) (Rompun ® %2, enjektabl solüsyon, Bayer, İstanbul) permedikasyonunu takiben ve 2 mg/kg dozunda ketamin hidroklorür (Ketasol %10, enjektabl solüsyon, Richter pharma, İstanbul) (IM) ile anesteziye alındı.…”

Section: Materyal Ve Metotunclassified

“…Bu çalışmada da elde edilen transfer edilebilir nitelikteki embriyoların gelişim aşamalarının yukarıda bildirilen literatürlerle paralellik gösterdiği tespit edilmiş ve uygulanan operasyon tekniği ve zamanlamasının Saanen keçileri için uygun bir metot olduğu kanısına varılmıştır. Türkiye'deki yerli ırklarda yapılan araştırmalarda, operasyonların 7. günde yapılması sonucunda daha fazla embriyo kaybının gözlendiği ve 6.5 günde yapılan operasyonların daha başarılı sonuçlar verdiği bildirilmiştir (1,36). Elde edilen bulgular eşliğinde Saanen keçilerinde benzer bir durumla karşılaşılmamıştır.…”

Section: Tartışma Ve Sonuçunclassified

In vivo elde edilen Saanen keçisi embriyolarının yavaş dondurulması üzerine farklı kriyoprotektanların etkisinin karşılaştırılması

GÜLER¹

2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada farklı kriyoprotektanların, yavaş dondurma yöntemi ile dondurulup çözdürülmüş Saanen keçisi embriyolarının canlılığı üzerine etkilerinin belirlenmesi amaçlanmıştır. Çalışmanın hayvan materyalini 15 baş Saanen ırkı keçi ve 3 baş teke oluşturdu. Keçilere siklusun dönemi gözetilmeksizin 12 gün süreyle fluorogeston asetat (20 mg) emdirilmiş intravaginal sünger yerleştirildi. Sünger uygulamasının 9. gününden itibaren 3 gün süreyle 12 saat arayla azalan dozlarda folikül uyarıcı hormon (FSH) (50-50; 30-30; 20-20 Comparision of different cryoprotectants on slow freezing of in vivo derived Saanen goats embryosSummary: This study aimed to determine the effects of different cryoprotectants on the viability of Saanen goats embryos which were frozen and thawed with slow freezing method. The study was conducted on 15 Saanen goats and 3 bucks. Fluorogeston acetate (20 mg) impregnated intravaginal sponges were inserted in goats for 12 days regardless of the sexual cycle. Starting on the 9 th day of intravaginal sponge administration, follicle stimulating hormone (FSH) was injected intramuscularly, every 12 hours at decreasing doses (50-50; 30-30; 20-20 mg) for 3 days. Goats were mated naturally 24 hours after removal of the sponges. Embryos were recovered by laparotomic uterine flushing on the 7th day after the first mating. Collected embryos were frozen by using 3 different cryoprotectants [ethylene glycol (EG), glycerol, and dimetil sulfoksit DMSO)] with slow freezing technique. Thawed embryos were incubated at 38.5 ºC and 5% CO2. The viability of embryos was evaluated at the 24 th , the 48 th , the 72 nd hours after thawing. Superovulation response (≥4 Cl), embryo recovery rate and transferable embryo rate were found to be 93.3%, 72.3% and 58%, respectively. Viability rates of frozen and thawed embryos at the 24 th , the 48 th , the 72 nd hours were found respectively to be 64.86%; 56.76%; 54.05% in EG group, 54.55%, 45.45%; 36.36% in glycerol group and 46.88%; 40.63%; 28.13% in DMSO group. Viability rates of the frozen embryos with EG were statistically better than embryos frozen with glycerol and DMSO (P<0.05) at 72 nd hour. Survival rates of blastocysts frozen were 76%; 64%; 60%; 54.6% in EG group 45.5%; 36.4% in glycerol group, and 42.1%; 36.8%; 21.1% in DMSO group and at 72 nd hour the difference between EG and DMSO group was significant (P<0.05). In conclusion, viability of embryos at the 24 th , the 48 th , the 72 nd hours after thawing in EG group was significantly higher than the embryos frozen with other cryoprotectants.

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“…On day 11 of the protocol, the intravaginal sponges were removed and on day 12, 4 µg of gonadotropin-releasing hormone (GnRH) (0.0042 mg/mL buserelin acetate; Receptal, Intervet, Germany) was injected by IM route in the morning. Four hours after the injection of GnRH, controlled natural mating was performed twice, once in the morning and evening (25). Oestrous synchronisation and superovulation were performed twice, in different breeding seasons.…”

Section: Oestrous Synchronisation and Superovulationmentioning

confidence: 99%