Assessment of Genetic Diversity in Bamboo Accessions of India Using Molecular Markers

Abstract

Bamboo is an important grass with wide scale applications in paper industries, medicines, constructions industries. It is potential feedstock for advanced biofuel production due to its favourable characteristics, natural abundance, rapid growth, perennial nature and higher CO2 sequestration. The objective of this study is to understand genetic diversity between the bamboo accessions with respect to geographical origin to correlate molecular information with feedstock characterization and adaptation to abiotic stress. In this study, genomic DNA was extracted from twenty bamboo accessions collected from different regions of India and genetic variations were assessed by inter simple sequence repeat (ISSR) based molecular marker approach using 8 primers. Maximum genetic distance was observed between Bambusa wamin-Itanagar & B. ventricosa-Durg (0.48221) & minimum genetic distance between Bambusa balcooa-Modasa & Bambusa balcooa-Tripura (0.00787). Bambusa balcooa and Bambusa vulgaris were genetically similar as compared to other accessions. The genetic distance is independent of geographical distance for the bamboo accessions considered in this study. The findings of this study will help to understand the degree of differences between bamboo accessions under the same environmental conditions and to identify the representative accessions that can be used for abiotic stress resistance studies. The information can be explored for screening of closely related bamboo accessions for abiotic stress resistance screening trials.Int J Appl Sci Biotechnol, Vol 3(2): 330-336 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12587

cpDNA-Gene-Sequence-Based Genetic Diversity, Population Structure, and Gene Flow Analysis of Ethiopian Lowland Bamboo (Bambusinea: Oxytenanthera abyssinica (A. Rich.) Munro)

“…Several morphological and molecular marker techniques such as random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR), amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR), expressed sequence-tag-derived simple sequence repeat (EST-SSR), sequence-related amplified polymorphism (SRAP), restriction fragment length polymorphism (RFLP), and interretrotransposon amplified polymorphism (IRAP) have been routinely used for genetic diversity study, population structure analysis, and characterization of bamboo germplasm [2,[40][41][42][43][44][45][46][47][48][49]. But so far, there is no study report on the molecular genetic diversity of O. abyssinica.…”

Wasteland Utilization for <i>B. balcooa</i> Cultivation: Socio-economic and Environmental Impacts through Bamboo-based Product Development

“…Moreover, energy pellet production from bamboo waste of incense sticks production can also generate additional indirect employment of around 8.66 million people considering 1.5 times of direct employment. Bamboo is globally considered as potential source of bioenergy due to its required fuel characteristics having calorific value of ~4000 kcal/kg which can be used for bio-pellets production that can replace alternative fuel like coal, lignite and furnace oil in boiler (FAO, 2016;Gami et al, 2015;. B. balcooa waste generated from incense sticks in energy pellets production would reduce 63.18 million tonnes/year consumption of coal with 96.12 million tonne/year CO2 reduction.…”