Assessment of genetic diversity in cucumber varieties using RAPD markers

Someh, Mahbube; Kiani, Ghaffar; Ranjbar, Gholam Ali; Alavi, S M

doi:10.37855/jah.2016.v18i01.14

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…RAPD has been used in genetic diversity assessment studies with many plant species (Singh et al, 2009;Youssef and Elazab 2015;Youssef et al, 2019), and proved to be an excellent tool to draw out variability and relationship among genotypes. Furthermore, RAPD has been used with cucumber to assess genetic diversity (Horejsi and Staub 1999;Someh et al, 2016;Yun et al, 2006), genetic stability of in vitro regenerated plants (Elmeer et al, 2009). In this regard, 118 cucumber accessions were analyzed using 71 RAPD loci (Horejsi and Staub, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…They reported that, RAPD is appropriate tool to use for studying genetic diversities of cucumber, which can help in parental selection and molecular marker-assisted selection in breeding procedures. Furthermore, Someh et al (2016) used 15 RAPD primer to assess the genetic diversity and relationship among 20 cucumber varieties. They found 73% polymorphism with 0.24 PIC value, in a context that this system could be used in selecting suitable parents in hybridization breeding programs in cucumber.…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity analysis for nine F1 hybrids of cucumber (Cucumis sativus L.) under Assuit conditions, Egypt

Mostafa

Mahmoud

Younes

2021

Archives of Agriculture Sciences Journal

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This experiment was performed on cucumber (Cucumis sativus L.) at Research Farm of the Faculty of Agriculture, Al-Azhar University (Assiut Branch), Assiut, Egypt (Longitude 031° 11' 09" E and latitude 27° 10' 39" N) in sandy clay loam soil. To evaluate nine F1 hybrids of cucumber by using molecular analysis. Cucumber hybrids were planted in March 2019 and 2020 for the summer seasons 2019 and 2020. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Randomly amplified polymorphic DNA was used to assess the genetic diversity and relationship among 9 cucumber hybrids. Five RAPD primers generated a total number of 34 bands, out of them 3 bands were polymorphic with 8.82% polymorphism. The total number of bands generated by each primer ranged from 5 to 9 bands with an average of 6.8 band per primer. Additionally, only two primers gave polymorphism among cucumber samples i.e., OPA-03 and OPA-08. Some genetic diversity measures were estimated based on RAPD molecular data. In this regard, the polymorphism information content (PIC) ranged for 0.05 (OPA-03) to 0.16 (OPA-08) with an average of 0.04 for all primers. The primer resolving power was 0.67 for OPA-03 and 1.11 for OPA-08, with an overall value of 1.78 for RAPD primers. In addition, diversity index was almost the same as PIC, with 0.04 (OPA-03) and 0.16 (OPA-08) and an average of 0.04 for all RAPD primers. .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis for nine F1 hybrids of cucumber (Cucumis sativus L.) under Assuit conditions, Egypt

Mostafa

Mahmoud

Younes

2021

Archives of Agriculture Sciences Journal

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“…Unlike the morphological and biochemical markers that could be much influenced with environmental factor and growth practices, DNA markers could portray genomic sequences composition thus enabling to detect genetic differences carried by different individuals (Xiao et al, 1996;Ovesna et al, 2002). Three of such useful markers system have been employed: (i) Random Amplified Polymorphic DNA (RAPD) uses an arbitrary, short primer that anneals to the genome at complementary sequences, and sizes of amplified products reveal existing polymorphisms (Nadeem et al, 2018) and have been applied successfully in diversity analyses of C. pepo (Athanasios et al, 2009;Ntuli et al, 2013;Mady et al, 2013;Someh et al, 2016) , (ii) ISSR markers (Zietkiewicz et al, 1994) are based on the amplification of DNA regions located between two microsatellites loci and used successfully in diversity analyses of C. pepo (Katzir et al, 2000;Heikal et al, 2008;Inan et al, 2012;Kiani and Siahchehreh, 2017) and (iii) Start Codon Targeted polymorphism (SCoT) is a gene targeted molecular marker technique derived from flanking ATG translation codon in plant gene and is considered to be more authentic in assessing genetic homogeneity (Xiong et al, 2011;Abdein et al, 2018). SCoT analysis has been also used to study genetic relationships in C. pepo (Xanthopoulou et al, 2015;Bhawna et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity in Summer Squash Genotypes Using some Yield Traits and DNA Markers Analysis under Sinai Conditions

El-Moneim¹,

Abdein²,

and³

2021

Journal of Plant Production Sciences

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Fourteen summer squash genotypes were used to assess their genetic diversity and relationships using some yield traits (Fruit length, fruit diameter, fruit shape index, average fruit weight, No of fruits/plant, fruit yield, and total soluble solids %) and DNA markers (Random Amplified Polymorphic DNA, inter-simple sequence repeat, Start Codon Targeted polymorphism) analyses. Two open field trials were executed at two locations, i.e., El-Arish (L 1 ) and Ras Sidr (L 2 ) in the North and South Sinai-Egypt, respectively. Significant differences were observed between both locations and indicated the superiority of (L 1 ) than (L 2 ) in all studied traits, while the interaction between genotype and location was significant for all studied traits. The location had a major effect on the relative genotypic potential of these traits, in which the genotype × location interaction was highly significant. SQ6 genotype ranked as the first in the fruit yield/plant and SQ2 for fruit diameter followed by SQ7 and SQ2 for fruit shape as well as SQ13 and SQ12 for the number of fruits/plant in L 1 and L 2 locations, respectively. Additionally, high polymorphism percentage (P %) was detected using RAPD (85%), ISSR (79.25%) and SCoT (69.44). Among the 15 studied primers, OP-B17 showed the maximum P% (100%) followed by primer OP-C17 (90.9 %) which also scored the highest values for polymorphism information content, effective multiplex ratio, and marker index. In addition, OP-K01 represented the maximum value of resolving power (12.56), indicated that these primers were highly informative. Intriguingly, 55 positive and two unique negative bands were amplified, out of which (33) bands were generated by RAPD followed by SCoT (15) and ISSR (9). In this regard, OP-C17 and SQ1 genotype produced the highest number of unique marker18 and 11amplicon, respectively. Moreover, the maximum similarity was found between SQ 11 and SQ 12 indicating that both genotypes were the most similar. Cluster and PCA analysis using RAPD, ISSR, and SCoT data grouped the 14 squash genotypes into four, three and two distinct clusters, respectively. Generally, the yield traits alongside molecular results showed significant information that may be utilized to sort and relationship between the studied summer squash genotypes under different conditions.

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SSR analysis to assess genetic diversity and population structure in parthenocarpy cucumber (Cucumis sativus L.)

Kaur,

Sharma,

Sharma

et al. 2023

J. Hortic. Sci.

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The genetic diversity and population relationship was determined in 14 genotypes of parthenocarpic cucumber (Cucumis sativus L.) using simple sequence repeats (SSR) markers. In this study, fifty-nine SSR markers comprehensively showed polymorphism among cucumber genotypes. Total 252 alleles were identified with an average of 4.27 alleles per locus, while the polymorphism information content (PIC) of the primers ranged from 0.34 to 0.84 with a mean value of 0.62. The major allele frequency and heterozygosity ranged from 0.21 to 0.75 and from 0.43 to 0.89, respectively. Maximum major allele frequency was reported with primer Cs- Female-4, whereas the maximum value of polymorphic information content was found with the primer SSR11742. The dendrogram clustered genotypes into two main groups A and B with 8 and 6 genotypes, respectively. Jaccard’s similarity coefficient ranged from 0.63 to 0.86 with maximum similarity between genotypes DDPCG3 and PLP-1, whereas minimum similarity was observed between DDPCG8 and PLP Gy-1-08B. The population structure revealed three sub-populations with some admixtures. Principal coordinate analysis (PCoA) with SSR markers revealed that the genotypes were uniformly distributed across the two axes in both the plots with 41.76% of cumulative variation. The genetic divergence within indigenous genotypes allow genotypic identification, gene mapping and cloning for improvement in cucumber breeding.

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Assessment of genetic diversity in cucumber varieties using RAPD markers

Cited by 3 publications

References 8 publications

Genetic diversity analysis for nine F1 hybrids of cucumber (Cucumis sativus L.) under Assuit conditions, Egypt

Genetic diversity analysis for nine F1 hybrids of cucumber (Cucumis sativus L.) under Assuit conditions, Egypt

Assessment of Genetic Diversity in Summer Squash Genotypes Using some Yield Traits and DNA Markers Analysis under Sinai Conditions

SSR analysis to assess genetic diversity and population structure in parthenocarpy cucumber (Cucumis sativus L.)

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