Assessment of genetic diversity in tomato landraces using ISSR markers

Henareh, M; Dursun, Atilla; Mandoulakani, Babak Abdollahi; Haliloğlu, Kamil

doi:10.2298/gensr1601025h

Cited by 30 publications

(26 citation statements)

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“…Inter simple sequence repeat (ISSR)-PCR is a simple and rapid technique that combines the advantages of SSR and AFLP with the universality of RAPD markers (Henareh et al, 2016). ISSR markers were developed to explore microsatellite repetitions without the need for DNA sequencing (Zietkiewicz et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

Castañeda-Cardona

Morillo-Coronado

Morillo

2020

Chil. j. agric. res.

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Dioscorea, commonly known as yam, is an economically, socially, and culturally-relevant tuber crop in many tropical countries. This study aimed to characterize the genetic diversity of Dioscorea alata L. and related species from Colombia (42 D. alata L., 6 D. bulbifera L., 3 D. rotundata Poir., and 3 D. trifida L. f.) through seven intersimple sequence repeat (ISSR) markers. The plant material was collected in five departments of Colombia. The seven ISSR generated 164 bands that showed high polymorphism (83.62%). Nei-Li genetic diversity coefficient (0.66) and Shannon information index (0.2636) revealed a high level of genetic diversity. The 54 genotypes were grouped into six groups according to geographic location but not by species. We found moderate genetic differentiation (Gst = 0.13) and high gene flow (N m = 2.7367). The analysis of molecular variance (AMOVA) showed higher variation (86%) within groups than among groups (14%). Our results suggest that the high genetic diversity in yam in Colombia can be exploited in future work on crop improvement.

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Section: Introductionmentioning

confidence: 99%

Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

Castañeda-Cardona

Morillo-Coronado

Morillo

2020

Chil. j. agric. res.

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“…Some of ISSR primers used to this research gave same percentage of polymorphism in other studies. (Kochieva et al, 2002;Korir et al, 2014), while (Vargas-Ponce et al, 2011;Henareh et al, 2016) obtained 100% polymorphism level from all primers they used. Generally polymorphic information content (PIC) of ISSR primers was high and 7 primers had over 60% PIC.…”

Section: Resultsmentioning

confidence: 99%

“…The first subpopulation included 18 accessions, while the second subpopulation comprised 14 accessions. (Henareh et al, 2016) assessed the genetic variation of Turkey and Iran tomato accessions. They determined two sub-populations by STRUCTURE analysis and genetic variation in sub-population 2 was higher than sub-population 1.…”

Section: Resultsmentioning

confidence: 99%

Determination of Genetic Relations among Tomato Accessions in Sulaymaniyah Region through ISSRs Markers Genetic Relations in Tomato Accessions

Ibrahim

Erdinç

2020

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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The goal of this study was to examine the genetic difference among thirty two tomato accessions (Solanum lycopersicum L.) grown in Sulaymaniyah using ISSR molecular markers technique. The results of ISSR markers revealed 65 amplified fragments, 50 of them were polymorphic from using 15 primers. Fifteen ISSR markers used to detect DNA polymorphism gave polymorphism percentage for each primer range between 50-100% with an average polymorphism percentage reaching 75.61%. ISSR6 and Sola 11 gave the highest polymorphism percentage was 100%, while 3F, Sola 5 and Sola 12 did not give any amplification. The mean of PIC (Polymorphic Information Content) value was 0.50 for ISSR markers. The similarity matrix was obtained by using Jaccard's coefficients, based on polymorphic bands and dendrogram constituted through UPGMA cluster analysis. The dendrogram revealed 4 main groups. Genetic similarity (GS) ranged from 0.261 to 0.941 within studied accessions. The highest similarity was 0.941 for the genotype pairs G7 and G16. However, the lowest similarity index was 0.261 among G15-G24 and G15-G26. Tomato accessions were determined by two populations according to STRUCTURE analysis. The ISSR marker system ensured useful information in describing genetic diversity among tomato accessions collected from different geographical around Sulaymaniyah province.

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“…The SDS-PAGE method provides maximum protein based diversity among genotypes; it is cheap and quick method then other molecular methods (ISEMURA et al, 2001;GEPTS and BLISS, 1988;IQBAL et al, 2005;JAVID et al, 2004;RAHMAN and HIRATA, 2004). Various biochemical and molecular methods are used to study genetic diversity among different crop species/sub-species (VERMA et al, 2015;GOODARZI et al, 2015;IZADPANAH et al, 2015;MARJANOVIC-JEROMELA et al, 2014;HENAREH et al, 2016).…”

Section: Genetic Similarity Matrix and Cluster Analysis Study Of B Rmentioning

confidence: 99%

Comparison of electrophoretic protein profiles of Brassica rapa sub-species brown sarson through SDS-PAGE method

Jan

Ashiq²,

Khurshid

et al. 2017

Genetika

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M. Ishaq Ibrahim, M. Adil, M. Ilyas (2017): Comparison of electrophoretic protein profiles of Brassica rapa sub-species brown sarson through Vol 49, No. 1,[95][96][97][98][99][100][101][102][103][104] Estimation of protein based variability among different Brassica sub-species is important for crop improvement. In present study total seed protein based variation among brown sub-specie of B. rapa was studied. Twenty different brown types' genotypes were analysed through Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) method. The small, medium and large sizes proteins were noted. A total of 12 bands were obtained in which 10 (83.33%) are highly polymorphic while the rest two (15.38%) are monomorphic. The protein size base polymorphism was divided into different groups on the basis of molecular weight that ranges from ~10 kDa to ~180 kDa. The data of variable proteins were analysed through Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method, which clustered all genotypes into four main cluster groups. The cluster I and II, III and IV consisted 3, 3, 6 and 10 genotypes respectively. The similarity coefficient values (40 to 96%) were calculated among different genotypes. The maximum similarity coefficient (96%) was recorded among genotypes Br-607, Br-560, and between Br-589 and Br-607 respectively. Our study showed maximum protein based diversity among brown sub-species of B. rapa which may serve as model to search protein based variation in other important plants sub-species.

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Assessment of genetic diversity in tomato landraces using ISSR markers

Cited by 30 publications

References 0 publications

Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

Determination of Genetic Relations among Tomato Accessions in Sulaymaniyah Region through ISSRs Markers Genetic Relations in Tomato Accessions

Comparison of electrophoretic protein profiles of Brassica rapa sub-species brown sarson through SDS-PAGE method

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