Assessment of Genetic Diversity in Four<i>Brassica</i>Species using Randomly Amplified Polymorphic DNA Markers

“…With the molecular approach, identification of particular plant accessions/ genotypes seems to be more effective as compared to traditional morphological marker system as it directly accesses the plants hereditary information and makes it possible to understand the genetic relationship between individuals (Paterson et al 1991). Random amplified polymorphic DNA (RAPD) marker system has been extensively used to distinguish the genetic variation at molecular level in several medicinal and aromatic plants (Ganie et al 2012;Kumar et al 2013;Khanna et al 2014;Prajapat et al 2015;Bhattacharyya and Kumaria 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers

et al. 2017

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Costus pictus, belonging to the family Costaceae, is one of the valuable medicinal plants with its antidiabetic property. Despite ever-increasing demand from the pharmaceutical industry, this species is being less exploited at molecular level. Hence, an effort has been made in the present study to characterize the 15 accessions of C. pictus collected from different geographical regions of India through random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. A total of 25 RAPD and 20 ISSR primers were used in the present study. The RAPD analysis generated 343 loci, of which 124 were polymorphic with an average of 4.96 loci per primer. While, ISSR primers produced 177 loci, of which 77 were polymorphic with an average of 3.85 loci per primer. The similarity coefficients ranged from 0.86-0.99, 0.84-0.95 and 0.86-0.96 for RAPD, ISSR and combined RAPD-ISSR, respectively. The UPGMA dendrogram generated using these data showed low level of divergence among the accessions from South and West regions. Further, accession-specific bands were also revealed by RAPD and ISSR markers which might be contributed to specific trait. This investigation was an understanding of genetic variation within the C. pictus accessions. The present finding indicates that both the marker tools RAPD and ISSR combined or individually can be used in determining the genetic relationship between the accessions. It may be concluded that data of hereditary differences appeared among the C. pictus accessions could be utilized for their conservation and reproducing programs.

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Random amplified polymorphic DNA and inter simple sequence repeat markers reveals genetic diversity between micro propagated, wild and field cultivated genotypes of Gloriosa superba: an endangered medicinal plant

Tilwari

Sharma

2021

Mol Biol Rep

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Assessment of Genetic Diversity in FourBrassicaSpecies using Randomly Amplified Polymorphic DNA Markers

Cited by 5 publications

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Study of Genetic Variability, Heritability and Genetic Advance in Sesame (Sesamum indicum L.) Genotypes

Study of Genetic Variability, Heritability and Genetic Advance in Sesame (Sesamum indicum L.) Genotypes

Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers

Random amplified polymorphic DNA and inter simple sequence repeat markers reveals genetic diversity between micro propagated, wild and field cultivated genotypes of Gloriosa superba: an endangered medicinal plant

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