Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers

Abstract

The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies less than 0.05. The NIFOR tenera parents recorded the highest number of rare alleles. The average observed heterozygosity and mean gene diversity across all parental groups were 0.6889 and 0.7029, respectively. Higher genetic diversity was detected among the NIFOR dura and tenera parents compared to that of the Deli dura parents in absolute terms. Analysis of molecular variance (AMOVA) showed that 87% of the total variation (p < 0.001) observed was due to differences among parents. Rogers' genetic distance ranged from 0.2988 to 0.8000 (mean = 0.5570). The dendrogram constructed on the basis of Rogers' genetic distance clustered the parents in three groups. They generally clustered in heterotic manner rather than by geographic origins. The groupings obtained through PCoA confirmed the results obtained by cluster analysis. The results obtained are strong assets for NIFOR breeding programme.

Assessment of genetic diversity in Fusarium wilt tolerant and susceptible oil palm (Elaeis guineensis Jacq) progenies in Nigeria using inter- simple sequence repeat (ISSR) molecular markers

“…and SCoT markers were higher than 0.345 and 0.298 when ISSR markers were used for the analysis on Vigna radiata and Vigna subterranea accessions. The PIC values identified in this study revealed that ten of the ISSR markers were able to identify the genetic diversity between the different oil palm progenies, which is in agreement with the study of Okoye et al (2016) which reported that the PIC values generated by SSR markers were helpful in the assessment of the diversity inequity between genotypes.…”

Genetic diversity in oil palm (Elaeis guineensis Jacq) using RAM (Random Amplified Microsatellites)

“…This value is similar to that reported by Khierallah et al (2011), who evaluated the genetic diversity of 30 oil palm cultivars with 22 microsatellite markers, finding a high level of polymorphism and an expected heterozygosity of 0.503 for all cultivars. Okoye et al (2016a) reported high heterozygosity and genetic diversity values of 0.69 and 0.70, respectively, when studying genetic diversity among NIFOR (Nigerian Institute For Oil palm Research) palm parentals with 10 microsatellite markers. Okoye et al (2016b) found average values of observed and expected heterozygosity of 0.63 and 0.76, respectively, when studying the genetic relationships between 26 oil palm materials from Nigeria and Malaysia with nine microsatellite markers.…”

Identication of Variance Moleculer Genotype Commercial Oil Palm (Elaeis guineensis Jacq.) based on Random Amplified Polymorpism DNA (RAPD) Markers

“…PIC values also was used to find the informative primers to see diversity. The PIC of RAPD have a maximum value of 0.5 [2]. A good RAPD primer can show a high percentage of polymorphic and a high score of PIC [23].…”

“…Genetic variance can be seen according to value of heterozygosity and value of homozygosity. If the value of heterozygosity is higher than the value of homozygosity, so that the genetic variance is high [2] [3]. If genetic variance between wild population compare with commercial oil palm can be found there were genetic depression.…”