Assessment of genetic diversity of cultivated and wild Iranian grape germplasm using retrotransposon-microsatellite amplified polymorphism (REMAP) markers and pomological traits

Razi, Mitra; Amiri, Mohamed Esmaeli; Darvishzadeh, Reza; Baneh, Hamed Doulati; Alipour, Hadi; Martínez‐Gómez, Pedro

doi:10.1007/s11033-020-05827-3

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…Oppositely to population structure analysis, the WPGMA analyses illustrated the separation clearly and divided the accessions under study into three groups which that of admixture accessions was located in distinguished group. This nding also con rmed by DAPC illustration which verify the existence of two heterotic groups in the studied A. desertorum germplasm with some overlaps which could be due to existence of some admixture accessions (Razi et al 2020).…”

Section: Discussionsupporting

confidence: 76%

Genetic variability and population structure of Agropyron desertorum accessions from Iran based on ISSR assay

Maleki

Mohammadi

Arshad

et al. 2023

Preprint

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The genus Agropyron as forage plant has several species which represented resistance against environmental stresses. DNA markers possessed a key role in assessment of plant germplasm and parental selection. In this regard, the genetic diversity of 34 A. desertorum accessions was studied using 28 ISSR primers. A total of 448 loci were amplified in A. desertorum genome that 402 (90%) out of them were polymorphic. The primer (AG)8YC produced the greatest number of polymorphic fragments while the primer (AC)8YA produced the lowest number. The number of effective alleles ranged between 1.26 and 1.57. The highest value of Shanon index was belonged to primer (GA)8T while the highest value of Nei index (0.33) was detected for primers (GA)8T, (TC)8C, and (GACA)4. Primer (GA)8GCC with PIC = 0.40 was the best informative marker in evaluation of A. desertorum genetic diversity. Genetic similarity among studied accessions was between 0.45 (G03 and G17) and 0.80 (G01 and G11). Using Bayesian and WPGMA classification algorithms, the A. desertorum germplasm was divided into two major subgroups (Red and Green) consisted of 13 and 17 accessions and also admixture accessions. Late mature accessions were distinguished from early mature accessions and mostly localized in Green subgroup. The Red subgroup had more private allele as well as heterozygosity compared with Green subgroup and therefore had also promising selection potential. Totally, ISSR markers could distinguished early mature genotypes and this is significant for more forage yield. Likewise, the recognized heterotic groups could help breeders to select highly polymorphic genotypes in polycross breeding.

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Section: Discussionsupporting

confidence: 76%

Genetic variability and population structure of Agropyron desertorum accessions from Iran based on ISSR assay

Maleki

Mohammadi

Arshad

et al. 2023

Preprint

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“…For the estimation of phenotypic variability, leaf and fruit descriptors have been widely used for both grapevine cultivars and clones [ 34 , 35 , 36 , 37 ]. Clones typically need multivariate biometric analysis to be distinguished [ 38 ]. Our work indicated heterogeneity in some morphological traits, such as rachis length and bunch compactness, which are under strong genetic control [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Taken together, these can be considered evidence of the common origin of the material. Therefore, it is possible to propose for further evaluation that the ‘Greco’ of the PDO area is of monoclonal origin [ 32 , 38 ]. This is also supported by the fact that divergent genotypes usually differed for very few loci.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Genetic Clonal Diversity within the ‘Greco Bianco’ Grapevine (Vitis vinifera L.) Variety

Villano

Corrado

Basile

et al. 2023

Plants

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Grapevine (Vitis vinifera L.) has been propagated vegetatively for hundreds of years. Therefore, plants tend to accumulate somatic mutations that can result in an intra-varietal diversity capable of generating distinct clones. Although it is common that winemakers request specific clones or selections for planting new vineyards, relatively limited information is available on the extent, degree, and morphological impact of the clonal diversity in traditional, highly valued grapevine varieties within production areas protected by geographical denomination of origin. Here, we present a morphological and genetic investigation of the intra-varietal diversity in ‘Greco Bianco’, the grapevine variety used to produce the DOCG and PDO “Greco di Tufo” wine. Seventeen clones from different farms (all within the allowed production area) were phenotypically characterized using ampelographic and ampelometric traits. The clones were also genotyped with Simple Sequence Repeats (SSR) and retrotransposon-based DNA markers (REMAP). The morphological analysis indicated a uniformity in the qualitatively scored traits, and a limited variability for the quantitative traits of the bunch and of the berry composition. The molecular markers also depicted variability among clones, which was more evident with the use of REMAPs. The comparison of the discriminatory information of the three analyses indicated that they provided different estimates of the level of diversity. The evaluation described herein of the clonal variability has implications for the management and protection of clonal selections in ‘Greco Bianco’ and prompts for further multidisciplinary investigations on its possible role in winemaking.

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Genetic variability and population structure of Agropyron desertorum accessions from Iran based on inter simple sequence repeat (ISSR) assay

Maleki

Mohammadi

Arshad

et al. 2023

Genet Resour Crop Evol

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Assessment of genetic diversity of cultivated and wild Iranian grape germplasm using retrotransposon-microsatellite amplified polymorphism (REMAP) markers and pomological traits

Cited by 6 publications

References 46 publications

Genetic variability and population structure of Agropyron desertorum accessions from Iran based on ISSR assay

Genetic variability and population structure of Agropyron desertorum accessions from Iran based on ISSR assay

Morphological and Genetic Clonal Diversity within the ‘Greco Bianco’ Grapevine (Vitis vinifera L.) Variety

Genetic variability and population structure of Agropyron desertorum accessions from Iran based on inter simple sequence repeat (ISSR) assay

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