Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis

Sharma, Vikas; Belwal, Nidhi S.; Kamal, Barkha; Dobriyal, A. K.; Jadon, Vikash S.

doi:10.1590/1678-4324-2016160389

Cited by 10 publications

(3 citation statements)

References 36 publications

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“…In vitro propagated plants are at risk of genetic variation (Sharma et al, 2016) and hence very important to assess their genetic fidelity (Min et al, 2010). RAPD, which is based on the non-coding regions of DNA, is a vital technique for efficient evaluation of genetic homogeneity and diversity (Abdelmigid and Morsi, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The RAPD marker technique has also been used to assess variation in other plant species, including Swertia chirayita (Roxb. ex Fleming) H. Karst (Sharma et al, 2016), Mucuna pruriens L. (Padmesh et al, 2006), Hystrix species (Zhang and Zhou, 2009), Chlorophytum borivilianum L. (Samantaray and Maiti, 2010), Dendrocalamus strictus (Roxb.) nees (Goyal et al, 2015), Picrorhiza kurroa Royle ex Benth (Rawat et al, 2010), and Magnolia sirindhorniae Noot.…”

Section: Discussionmentioning

confidence: 99%

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A Micropropagation Protocol for the Endangered Medicinal Tree Prunus africana (Hook f.) Kalkman: Genetic Fidelity and Physiological Parameter Assessment

Komakech

Kim

et al. 2020

Front. Plant Sci.

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Prunus africana is an endangered medicinal plant and hence new propagation methods are urgently required to increase its populations. Unfortunately, propagation through seeds is challenging due to its long flowering cycle and recalcitrant seeds. We developed a protocol for micropropagation using nodal segment explants. A woody plant medium supplemented with vitamins, 15 g L−1 sucrose, and 1.0 mg L−1 6-benzylaminopurine (BAP) supported the optimum rate (100%) of axillary shoot initiation. Supplementation with 15 g L−1 sucrose and 1.5 mg L−1 indole-3-acetic acid (IAA) provided the optimum rate (75%) of root initiation. Rooted plantlets were successfully planted in sterilized horticultural soil containing perlite (2:1 v/v) and the survival rate was 98% following acclimatization. The photosynthetic rate assessed using FlourPen FP110 series showed that the ratio of variable fluorescence to maximum fluorescence mean value for in vitro regenerated P. africana (0.830 ± 0.0008) was similar to that of the maternal P. africana plant (0.825 ± 0.005), indicating similarity in their photosynthetic performance; a pivotal process for growth and development. The Fourier transform near-IR (FT-NIR) spectrometer analysis of the in vitro regenerated and the maternal P. africana plant samples exhibited homogeneity in the absorbance peaks at 8,273, 6,344, and 4,938–4,500 cm−1 associated with lipids, starch, and proteins. The genetic fidelity of regenerated plants was confirmed using the randomly amplified polymorphic DNA (RAPD) technique. Our protocol is suitable for use in large-scale P. africana to meet the increasing demands for it in the global market.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Micropropagation Protocol for the Endangered Medicinal Tree Prunus africana (Hook f.) Kalkman: Genetic Fidelity and Physiological Parameter Assessment

Komakech

Kim

et al. 2020

Front. Plant Sci.

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“…Cineraria maritime [67], etc. The RAPD markers can generate a higher number of bands, thereby constructing an additional representative sample of the genome than is possible with allozyme [58]. Moreover, RAPD provides numerous benefits over restriction fragment length polymorphism (RFLP) in various ways like no previous cloning of DNA sequences is obligatory, requires very little amount of plant tissue, and is associated with a rapid DNA extraction procedure [26,51].…”

Section: Rapd Analysismentioning

confidence: 99%

Clonal fidelity investigation of micropropagated hardened plants of jackfruit tree (Artocarpus heterophyllus L.) with RAPD markers

Kader

Sinha

Ghosh

2022

Journal of Genetic Engineering and Biotechnology

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Background Artocarpus heterophyllus is an important tropical agroforestry species that bears multiple applications. However, the population of this species is reduced due to various anthropogenic activities. For this reason, in vitro approach is needed to propagate or conserve this species as in vivo propagation methods face various obstacles. In this respect, the present investigation was undertaken to produce genetically stable jackfruit trees through in vitro technology. In vivo grew shoot tips were harvested on Murashige and Skoog (MS) medium containing several plant growth regulators to achieve this. Results The 6-benzylaminopurine (BAP) at the concentration of 1.5 mg L-1, indole-3-butyric acid (IBA) at 0.5 mg L-1, and α-naphthaleneacetic acid (NAA) at 0.1 mg L-1 in combination on MS media yielded superior shoot response (94.44%), longest shoot length (4.02 cm), and the maximum number of shoots per explant (4.78). They were further multiplied by repeated subculturing on the same media composition and the third subculture resulted in a maximum number of shoots (5.92) with the largest shoot length (5.85 cm). Among the different media screened for rooting, the ¼ MS media yielded 94.44% rooting response, the longest root length (3.78 cm), and the maximum number of roots per shoot (8.44) with 0.1 mg L-1 NAA, 0.5 mg L-1 IBA and 0.1 mg L-1 BAP in combination. Primary hardening showed 88.89% of plant survival under greenhouse conditions after 4 weeks of incubation having a sterilized mixture of garden soil and vermiculite mixture (1:1, w/w). It increased to 90.60% after the secondary hardening process in a vermiculite-soil mixture (2:1; w/w). No polymorphism was detected on random amplification of polymorphic DNA (RAPD) profiling between the mother plant and hardened plants, indicating high genetic stability among the clones. Conclusions This is the first report of the genetic fidelity study of in vitro grown regenerants of A. heterophyllus. This study established a micropropagation protocol for genetically uniform in vitro regeneration of this species to supply plant resources to various industries or conservation of elite germplasm.

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Genetic Fidelity Studies for Testing True-to-Type Plants in Some Horticultural and Medicinal Crops Using Molecular Markers

Tyagi¹,

Rajpurohit

Sharma³

2021

Agricultural Biotechnology: Latest Research and Trends

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Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis

Abstract: An efficient in vitro regeneration protocol for medicinally important herb

Cited by 10 publications

References 36 publications

A Micropropagation Protocol for the Endangered Medicinal Tree Prunus africana (Hook f.) Kalkman: Genetic Fidelity and Physiological Parameter Assessment

A Micropropagation Protocol for the Endangered Medicinal Tree Prunus africana (Hook f.) Kalkman: Genetic Fidelity and Physiological Parameter Assessment

Clonal fidelity investigation of micropropagated hardened plants of jackfruit tree (Artocarpus heterophyllus L.) with RAPD markers

Genetic Fidelity Studies for Testing True-to-Type Plants in Some Horticultural and Medicinal Crops Using Molecular Markers

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