Assessment of genetic homogeneity and analysis of phytomedicinal potential in micropropagated plants of Nardostachys jatamansi, a critically endangered, medicinal plant of alpine Himalayas

Insights into nuclear DNA content, hydrogen peroxide and antioxidative enzyme activities during transverse thin cell layer organogenesis and ex vitro acclimatization of Malaxis wallichii, a threatened medicinal orchid

“…Effectiveness of ISJ markers in detecting clonal fidelity in in vitro system was previously reported by us in Nardostachys jatamansi (D. Don) DC. (Bose et al 2016). In the present investigations, mT was found to be less toxic at higher equimolar concentration compared to other conventional cytokinins which could generate variations (phenotypic and genetic) during in vitro culture as supported by findings of other workers (Werbrouck et al 1996;Bairu et al 2007;Amoo et al 2011;Gentile et al 2014;Bhattacharyya et al 2016).…”

“…Furthermore, its faster translocation rate and reversible sequestration of the o-glucoside at a physiologically active level over an extended period of time in plant tissue resulting in much higher multiplication rate justifies its ingenuity over other purine based cytokinins in plant tissue culture (Amoo et al 2014;Valero-Aracama et al 2010). The superiority of mT and its derivatives in improving shoot proliferation over the conventional cytokinins like BAP and Kn was demonstrated by some researchers in recent past (Amoo and Van Staden 2013;Aremu et al 2012;Bairu et al 2007;Baroja-Fernández et al 2002;Bose et al 2016;Gentile et al 2014;Roels et al 2005). However, a few reports on the use of mT in orchid tissue culture indicate that this group of topolins could be a new source of aromatic cytokinins with high multiplication efficiency and improved physiological activity in micropropagated plants (Vasudevan and Van Staden 2011;Bhattacharyya et al 2016).…”

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

“…The addition of 30 g/L sucrose was found to be the optimum for the growth of Thymus hyemalis compared to other types of carbon sources including glucose, fructose, mannitol and sorbitol [ 17 ]. The studies implied that a sucrose concentration higher than 30 g/L results in a decrease in the multiplication and growth rate of plants [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. These findings were in agreement with the present study in which 30 g/L of sucrose was established as the optimum for the growth of C. nutans .…”

In vitro plant tissue culture: means for production of biological active compounds

“…The methodologies involved in that process include the use of several techniques to assess possible alterations at different levels (Devarumath et al 2002;Bhattacharyya et al 2015Bhattacharyya et al , 2017aBose et al 2016;Bhattacharyya and Van Staden 2016). Flow cytometry and chromosome counting are widely used to assess changes in ploidy and chromosome number; PCRbased DNA markers, random amplified polymorphic DNA (RAPD), random fragmented length polymorphism (RFLP), inter simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), microsatellite markers, and start codon targeted (ScoT) polymorphism have been successfully used to evaluate genomic stability of regenerated plants (Hu et al 2008;Collard and Mackill 2009;Bairu et al 2011;Singh et al 2013;Bhattacharyya et al 2014Bhattacharyya et al , 2015Rathore et al 2014;Stanišic et al 2015;Bose et al 2016;Govindaraju and Arulselvi 2016). An appropriate combination of two or more markers guarantees reliable and efficient testing of genetic fidelity in plants (Palombi and Damiano 2002;.…”