Assessment of Genetic Relationships among Isolates of Macrophomina phaseolina Using a Simplified AFLP Technique and Two Different Methods of Analysis

Vandemark, George J.; Vega, Octavio Martínez de la; Pecina, Victor; Alvarado, Maria de Jesus

doi:10.2307/3761423

Cited by 27 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of the present study clearly demonstrated that M. phaseolina from different parts of the country were highly variable and ISSR markers are suitable to reflect the genetic diversity among the populations. A number of molecular studies elsewhere have shown a high level of polymorphism in this fungus when isolates from different host or geographical origins were compared using different molecular tools (Almeida et al, 2003;Jones et al, 1998;Mayek-Perez et al, 2001;Su et al, 2001;Vandemark et al, 2000). Other recent studies have demonstrated the genetic diversity among M. phaseolina isolates (Aboshosha et al, 2007;Aghakhani and Dubey, 2009;Babu et al, 2010;Baird et al, 2010;Beas-Fernandez et al, 2006;Das et al, 2006;Jana et al, 2005a;Jana et al, 2005b;Omar et al, 2007;Purkayastha et al, 2008;Rajkumar and Kuruvinashetti, 2007;Reyes-Franco et al, 2006;Saleh et al, 2010).…”

Section: Resultsmentioning

confidence: 98%

“…However, usually isolates from the same host or origin tend to group together. Several studies have found no association between DNA genotype and host origin in populations (Almeida et al, 2003;Reyes-Franco et al, 2006;Vandemark et al, 2000). Recently Saleh et al (2010) used AFLP markers and ITS region sequences to assess the genetic diversity and relationships of M. phaseolina isolates from maize, sorghum, tallgrass prairie and soybean.…”

Section: Resultsmentioning

confidence: 99%

“…Different molecular methods have been used for differentiating M. phaseolina populations including Restriction Fragment Length Polymorphism (RFLP) of rDNA-ITS regions (Aghakhani and Dubey, 2009;Almeida et al, 2003;Purkayastha et al, 2006;Su et al, 2001), Random Amplified Polymorphic DNA (RAPD) (Aboshosha et al, 2007;Aghakhani and Dubey, 2009;Almeida et al, 2003;Almeida et al, 2008;Babu et al, 2010;Das et al, 2006;Jana et al, 2003;Omar et al, 2007;Purkayastha et al, Rajkumar and Kuruvinashetti, 2007;Su et al, 2001;Zade et al, 2009), Amplified Fragment Length Polymorphism (AFLP) (Brooker et al, 2008;Mayek-Perez et al, 2001;Reyes-Franco et al, 2006;Saleh et al, 2010;Vandemark et al, 2000), Universal Rice Primer PCR (URP-PCR) (Jana et al, 2005b), Inter simple sequence repeats (ISSR) (Jana et al, 2005a;Purkayastha et al, 2008), Repetitive SequenceBased Polymerase Chain Reaction (Rep-PCR) (Purkayastha et al, 2008) and SSR (Baird et al, 2010). Inter simple sequence repeat (ISSR) markers are powerful tools which can be utilized to access the variation in the flanking regions of microsatellite loci that are dispersed throughout all genomes (Zietkiewicz et al, 1994).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Mahdizadeh¹,

Safaie²,

Goltapeh³

2011

The Plant Pathology Journal

View full text Add to dashboard Cite

Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at 30°C and 37°C, average size of microsclerotia, and time to microsclerotia formation. The feathery colony phenotype was the most common (63.7%) on the chlorate selective medium and represented the chlorate sensitive phenotype of the Iranian Macrophomina phaseolina population. Meantime, inter simple sequence repeats (ISSR) Markers were used to assess the genetic diversity of the fungus. Unweighted pair-group method using arithmetic means (UPGMA) clustering of data showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins, however, usually the isolates from the same host or the same geographic origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and phenotypic characteristics. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the phenotypic and the genotypic characteristics investigated and needs more studies using neutral molecular tools to get a deeper insight into this complex species.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Mahdizadeh¹,

Safaie²,

Goltapeh³

2011

The Plant Pathology Journal

View full text Add to dashboard Cite

show abstract

“…In the present study, examination of a large sample of M. phaseolina isolates for genetic diversity, host isolates collected from eight major agro-climatic zones in India. Various studies devoted to the genetic, geo-diversity and pathogenic variability of M. phaseolina collected from Mexico and other countries have shown clear differentiation [4,5,13]. Jana et al [14] developed taxonomic marker for population studies by using a single RAPD primer that distinguishes isolates of M. phaseolina from soybean, sesame, ground nut, chickpea, cotton, common bean and 13 other hosts.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Macrophomina phaseolina isolates from certain agro-climatic regions of India by using RAPD markers

et al. 2010

View full text Add to dashboard Cite

Genetic diversity analysis of Macrophomina phaseolina isolates obtained from different host range and diverse geographical locations in India was carried out using used, primer OPB-08 gave the maximum polymorphism and the UPGMA clustering could separate 50 isolates in to ten groups at more than 65% similarity level. The ten clusters correlated well with the geographical locations with exceptions for isolates obtained from Eastern and Western Ghats. There was a segregation of isolates from these two geographical locations in to two clusters thus, distributing 10 genotypes in to eight geographical locations. All the isolates M. phaseolina irrespective of their host and geographical origin, exhibited two representative monomorphic bands at 250 bp and 1 kb, presence of these bands suggests that isolates might have evolved from a common ancestor but due to geographical isolation fallowed by natural selection and genetic drift might have segregated in to subpopulations. dispersal of single lineage in all locations in India.

show abstract

“…The genetic diversity of M. phaseolina could favor its survival and adaptation to variable environments, because significant morphological (Mayek-Pérez et al, 2001), physiological (Mihali and Taylor, 1995), pathogenic (Mayek-Pérez et al, 2001;Su et al, 2001), and genetic (Vandemark et al, 2000;Mayek-Pérez et al, 2001;Su et al, 2001;Almeida et al, 2003;Jana et al, 2003;Aboshosha et al, 2007) diversity has been reported. However, no clear evidence to suggest formae specials subspecies or physiological races has been reported, although Su et al (2001) suggested host specialization in the genus.…”

mentioning

confidence: 99%

Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina

Rayatpanah

Nanagulyan

Alav

et al. 2012

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is an economically important disease of oilseed plants in Northern Iran. Seventy isolates of M. phaseolina were obtained from different hosts, including soybean (Glycine max L.), and sunflower (Helianthus annuus L.) in the northern oilseed planting regions of Iran. RAPD-PCR amplification profiles, by using of six random OPA primers (kit A) showed polymorphisms among the isolates. The primer OPA-13 (5' _ CAGCACCCAC _ 3') amplified the genomic DNA of all isolates of M. phaseolina producing 4-12 bands of sizes between 0.25 and 2.3 kb. Unweighted pair-group method with arithmetic mean (UPGMA) analysis classified the isolates into the nine major groups with 64% similarity. Pathogenicity of isolates was evaluated at seedling stage of soybean, sunflower, and maize (Zea mays L.) plants under in vitro conditions. None of the isolates were pathogenic on corn, while all of the isolates infected soybean and sunflower seedlings. Isolates were more virulent on soybean than sunflower. The disease index on sunflower and soybean varied between 19-24 and 27-30 respectively. These results indicated a significant pathogenic and genetic variability within the Iranian isolates of M. phaseolina. Cultivation with crop rotation was probably tended to induce less diversity of the pathogen isolates.

show abstract

Assessment of Genetic Relationships among Isolates of Macrophomina phaseolina Using a Simplified AFLP Technique and Two Different Methods of Analysis

Cited by 27 publications

References 32 publications

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Genetic diversity of Macrophomina phaseolina isolates from certain agro-climatic regions of India by using RAPD markers

Pathogenic and Genetic Diversity among Iranian Isolates of Macrophomina phaseolina

Contact Info

Product

Resources

About