Assessment  of  Genetic  Variability  Among  Raspberry Accessions  Using  Molecular  Markers

Simlat, Magdalena; Ptak, Agata; Kula, Adam; Orzeł, A.

doi:10.24326/asphc.2018.5.6

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…Earlier, PCA of 50 Rubus cultivars based on genomic SSRs showed that black raspberry is the most distant from other Rubus species [60]. Interestingly, in report of Simlat et al [71], Jewel cultivar was not separated from red raspberry using PCA based on SSRs. According to our data, Rubus hybrids clustered together with red raspberry, while in Graham et al [60] they were located approximately in the middle between red raspberry and blackberry cultivars.…”

Section: Genetic Diversity Of Fragaria and Rubusmentioning

confidence: 95%

Transferability and Polymorphism of SSR Markers Located in Flavonoid Pathway Genes in Fragaria and Rubus Species

Lebedev

Subbotina

Maluchenko

et al. 2019

Genes

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Strawberry (Fragaria) and raspberry (Rubus) are very popular crops, and improving their nutritional quality and disease resistance are important tasks in their breeding programs that are becoming increasingly based on use of functional DNA markers. We identified 118 microsatellite (simple sequence repeat-SSR) loci in the nucleotide sequences of flavonoid biosynthesis and pathogenesis-related genes and developed 24 SSR markers representing some of these structural and regulatory genes. These markers were used to assess the genetic diversity of 48 Fragaria and Rubus specimens, including wild species and rare cultivars, which differ in berry color, ploidy, and origin. We have demonstrated that a high proportion of the developed markers are transferable within and between Fragaria and Rubus genera and are polymorphic. Transferability and polymorphism of the SSR markers depended on location of their polymerase chain reaction (PCR) primer annealing sites and microsatellite loci in genes, respectively. High polymorphism of the SSR markers in regulatory flavonoid biosynthesis genes suggests their allelic variability that can be potentially associated with differences in flavonoid accumulation and composition. This set of SSR markers may be a useful molecular tool in strawberry and raspberry breeding programs for improvement anthocyanin related traits.

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Section: Genetic Diversity Of Fragaria and Rubusmentioning

confidence: 95%

Transferability and Polymorphism of SSR Markers Located in Flavonoid Pathway Genes in Fragaria and Rubus Species

Lebedev

Subbotina

Maluchenko

et al. 2019

Genes

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“…The cultivar 'Laszka' was developed by crossing clones 80408 × 80192 in the Experimental Station of the Research Institute of Horticulture in Brzezna. On 8 February 2006, it was listed in the National Register of Horticultural Plant Varieties [107,108]. The cultivar 'Glen Ample' is a licensed Scottish variety developed in 1978 at the Scottish Institute of Plant Production in Dundee, UK.…”

Section: Origin Of Cultivarsmentioning

confidence: 99%

Nutritionally Important Pro-Health Active Ingredients and Antioxidant Properties of Fruits and Fruit Juice of Selected Biennial Fruiting Rubus idaeus L. Cultivars

Chwil,

Matraszek-Gawron,

Kostryco

et al. 2023

Pharmaceuticals

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Raspberry fruits are an important source of many biologically active chemical compounds exerting nutritional and pro-health effects. The study presents a comparative analysis of nutritionally important bioactive chemical compounds—polyphenols; flavonoids, including anthocyanins; vitamin C; amino acids; fatty acids; and primary metabolites—contained in the fruits of three biennial fruiting cultivars, R. idaeus ‘Glen Ample’, ‘Laszka’, and ‘Radziejowa’, i.e., common cultivars in Poland and Europe. The antioxidant activity of fresh fruits and juice was determined with five methods. The analyses revealed the strong free radical scavenging potential of the fruits and juice, confirmed by the high concentration of nutrients, e.g., polyphenols, anthocyanins, vitamin C, amino acids, and fatty acids. The antioxidant activity of the juice determined with the ferric reducing antioxidant power (FRAP) and OH radical methods was from 2.5 to 4.0 times higher than that of the fruits. The following orders of total polyphenol contents were established in the analyzed cultivars: ‘Glen Ample’ < ‘Laszka’ < ‘Radziejowa’ in the fruits and ‘Glen Ample’ < ‘Radziejowa’ < ‘Laszka’ in the juice. The highest antioxidant activity was exhibited by the ‘Radziejowa’ fruits. Given their high content of dietary fiber, the fruits of the analyzed raspberry cultivars can be consumed by dieting subjects. The concentrations of vitamin C (28–34 mg/100 g) and anthocyanins (20–34 mg/100 g) indicate the biological and pharmacological activity of these fruits. The main unsaturated fatty acids in the fruits were gamma-linoleic acid (C18:2n6c) and alpha-linolenic acid (C18:3n3), which neutralize excess free radicals. The amino acids nutritionally essential to humans were dominated by leucine, arginine, and phenylalanine. This is the first comparative analysis of the antioxidant activity of fruits and juice and the contents of selected active compounds in the fruits of biennial fruiting cultivars of R. idaeus, i.e., a highly commercialized crop in Europe.

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“…OPA-03-AGTCAGCCAC, OPA-11-CAATCGCCGT, OPA-13-CAGCACCCAC, OPA-19-CAAACGTCGG. Amplification, visualization and analysis of obtained DNA fragments were performed according to Simlat et al [36].…”

Section: Dna Analysismentioning

confidence: 99%

Enrichment of Different Plant Seeds with Zinc and Assessment of Health Risk of Zn-Fortified Sprouts Consumption

et al. 2020

Self Cite

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Zinc (Zn) is a nutrient that regulates many vital functions of the human body, hence the demand for Zn in the balanced daily nutrition has to be covered. Priming of seeds with Zn for Zn-enriched sprout production can be considered a good alternative to artificial supplementation in a modern diet. Hence, the aim of our study was to determine the exposure level of Zn bringing neither risk for humans nor growth inhibition of enriched broccoli, pea and sunflower sprouts. Seeds treated with 0, 10, 20 and 30 μg mL−1 ZnSO4 responded in a differentiated way to Zn. Pea seed germination and sprout growth was diminished by 30 μg mL−1 ZnSO4, but for sunflower sprouts this Zn level resulted in the highest fresh mass and largest hypocotyls. Zn content in sprouts greatly increased in a dose-dependent manner, mostly in broccoli (up to 25 times) and peas (up to 4 times), and to a lesser extent (up to 120%) for sunflowers. Free radical scavenging activity was usually decreased. Considering the potential non-carcinogenic risk of sprouts consumption estimated by the hazard analysis, the safest exposure level for seed priming will be 10 μg mL−1 ZnSO4. It was confirmed by random amplification of polymorphic DNA analyses indicating no DNA variations in sprouts treated with 10 μg mL−1 ZnSO4 compared to the control.

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Assessment of Genetic Variability Among Raspberry Accessions Using Molecular Markers

Cited by 9 publications

References 17 publications

Transferability and Polymorphism of SSR Markers Located in Flavonoid Pathway Genes in Fragaria and Rubus Species

Transferability and Polymorphism of SSR Markers Located in Flavonoid Pathway Genes in Fragaria and Rubus Species

Nutritionally Important Pro-Health Active Ingredients and Antioxidant Properties of Fruits and Fruit Juice of Selected Biennial Fruiting Rubus idaeus L. Cultivars

Enrichment of Different Plant Seeds with Zinc and Assessment of Health Risk of Zn-Fortified Sprouts Consumption

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