The present investigation aimed at to evaluate twenty rice genotypes including Sub 1 introgressed lines along with their parents and four check varieties namely Lalat, Pratikshya, Mahanadi and PSBRc-68. The trial was laid out in randomized block design with three replications. Different yield attributing traits, physiological parameters and biochemical traits were examined to study the availability and extent of genetic variability and to assess the physiological and genetic bases of yield variations in Sub-1 introgressed lines and their parental strains. The analysis of variance showed significant variation among the test genotypes for all the traits except panicle number. Moderate to high degree of heritability estimates were observed for all the traits except for panicle number and leaf area index. The characters like chlorophyll content, carbohydrate content, Super oxide dismutase content, catalase content, glutathione peroxidase content, different growth parameters, grain yield per plant and plot yield exhibited high genotypic coefficient of variation coupled with high heritability and high genetic advance. The genotypes like Swarna, Ciherang,