Assessment of genotoxicity of waterpipe smoking using 8-OHdG biomarker

Abstract

ABSTRACT. Waterpipe tobacco smoking is increasing in popularity, particularly among young adults. This popularity is related to the lack knowledge regarding the health effects of waterpipe smoking. In this study, we examined the genotoxicity of waterpipe smoking using an 8-hydroxy deoxyguanosine (8-OHdG) assay. Genotoxicity was evaluated in the saliva, urine, and serum of 66 waterpipe adult smokers and 46 healthy nonsmokers. The level of addiction to waterpipe smoking was evaluated using the Lebanon Waterpipe Dependence Scale-11. Levels of 8-OHdG in the samples were measured using commercially available enzyme-linked immunosorbent assays. Levels of 8-OHdG in the saliva (52,430 ± 2923 vs 48,430 ± 4189 pg/mL), urine (2815 ± 312 vs 2608 ± 180 pg/mL), and serum (19,720 ± 202 vs 19,670 ± 254 pg/mL) were similar between waterpipe smokers and nonsmokers (P > 0.05). In addition, no correlations were found between dependence M. Azab et al. 9556©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9555-9561 (2015) score and levels of 8-OHdG in all sample types. In conclusion, 8-OHdG is not a good biomarker for genotoxic effect of waterpipe smoking.

Waterpipe Tobacco Chemical Content, Microbial Contamination, and Genotoxic Effects: A Systematic Review

“…The search generated 1,286 scientific articles; title and abstract screening resulted in the selection of 27; and in the screening of the full texts, 22 studies met the inclusion criteria. [9][10][11]13,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] A flowchart of the search and selection process is displayed in Figure 1. Of the 22 included studies, 14 studies explored the chemical families and components coming from the waterpipe smoke, [9][10][11][18][19][20][21][22][23][24][25][26][27][28] 3 studies assessed the microbial contaminants of waterpipe smoke, [29][30][31] and 5 studies evaluated the genotoxic effects of waterpipe smoke.…”

“…Of the 22 included studies, 14 studies explored the chemical families and components coming from the waterpipe smoke, [9][10][11][18][19][20][21][22][23][24][25][26][27][28] 3 studies assessed the microbial contaminants of waterpipe smoke, [29][30][31] and 5 studies evaluated the genotoxic effects of waterpipe smoke. 13,[32][33][34][35] Chemical Families and Components…”

Plasma and saliva levels of three metals in waterpipe smokers: a case control study

“…Tubes were mixed immediately and then centrifuged at 1500 xg for 5 minutes to obtain plasma. Saliva samples were collected as described previously (Azab et al 2015). In brief, participants rinsed their mouth three times with distilled water and, 5 minutes after, about 2 mL of saliva was collected.…”

Vitamin B12 protects against DNA damage induced by hydrochlorothiazide

“…Using light microscope (at high-resolution; final magnification: 1000×; from Nikon, Japan), all slides were scored for SCE. A minimum of fifty-clearly differentiated metaphases containing 42–46 chromosomes were scored for every cell culture as previously described (Azab et al, 2015).…”